A kit optimized to efficiently differentiate pluripotent cells into dopaminergic neurons under serum-free conditions.
Pre-optimized kit reduces experimental variation
Defined conditions decreases variation
Includes a panel of premium quality growth factors
Why Use Pre-optimized Reagents to Differentiate Pluripotent Stem Cells into Dopaminergic Neurons?
The loss of dopaminergic neurons in the substantia nigra is a characteristic of Parkinson’s disease (PD), a neurodegenerative disorder that affects motor function.
Both animal models and clinical trials have suggested that cell replacement therapies may be effective in treating PD. However, this approach is limited by the availability of a rich, effective source of neural precursors for dopaminergic neuron generation. While embryonic and induced pluripotent stem cells can be differentiated into dopaminergic neurons, successful differentiation is dependent on high quality media and defined differentiation supplements. R&D Systems offers the Human/Mouse Dopaminergic Neuron Differentiation Kit to drive successful and efficient differentiation of embryonic and induced pluripotent stem cells into dopaminergic neurons.
R&D Systems Human/Mouse Dopaminergic Neuron Differentiation Kit:
Includes high quality reagents to generate dopaminergic neurons in 27 days.
Includes pre-optimized components with low lot-to-lot variation.
Includes sufficient reagents for differentiation of 3 x 107 pluripotent stem cells.
The Human/Mouse Dopaminergic Neuron Differentiation Kit contains ITS and N-2 MAX Media Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to support cell attachment and spreading. A growth factor panel, consisting of Recombinant Human Fibroblast Growth Factor Basic (FGF basic), Recombinant Mouse Fibroblast Growth Factor 8b (FGF-8b) and Recombinant Mouse Sonic Hedgehog Amino-terminal Peptide (Shh-N), is included for effective dopaminergic differentiation.
The quantity of each component provided in the kit is estimated to be sufficient for differentiation of 3 x 107 embryonic stem cells.
Recombinant Mouse FGF-8b
Recombinant Human FGF basic
Recombinant Mouse Shh-N
Purified Bovine Fibronectin
ITS Media Supplement
N-2 MAX Media Supplement
Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.
To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.
Human/Mouse Dopaminergic Neuron Differentiation Kit Scientific Data Examples
Characterization of Dopaminergic Neurons Generated from Human Pluripotent Stem Cells.
Dopaminergic neurons were generated from human pluripotent stem cells using the Dopaminergic Neuron Differentiation Kit (Catalog # SC001B). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566). The cells were stained with the NorthernLights™(NL) 557-conjugated Donkey Anti-Mouse Antigen Affinity-purified Secondary Antibody (Catalog # NL007, red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (Catalog # MAB1195) followed by the NL493-flourochrome-conjugated Donkey Anti-Mouse Antigen Affinity-purified Secondary Antibody (Catalog # NL009, green). View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation & Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Refer to the product datasheet for complete product details.
Briefly, pluripotent stem cells are differentiated into dopaminergic neurons using the following procedure:
Generate embryoid bodies from pluripotent cells
Select for and expand Nestin positive cells
Differentiate Nestin positive cells into dopaminergic neurons
Can the Human/Mouse Dopaminergic Neuron Differentiation Kit work with a starting population of neural progenitor cells instead of mouse pluripotent stem cells?
The kit may work with a starting population of neural progenitor cells, but we have not tested this. If starting with neural progenitor cells we recommend starting at Stage 5 of the Dopaminergic Neuron Differentiation Kit protocol. The starting cell density would also have to be optimized.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics