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StemXVivo EMT Inducing Media Supplement (100X)

Induction of Epithelial to Mesenchymal Transition(EMT) in the A549 human lung carcinoma cell line.
(2)
Upregulation of the Mesenchymal Markers, Snail and Vimentin, in EMT-induced Cells. 
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CCM017
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, an epithelial to mesenchymal transition (EMT) is induced using the following in vitro differentiation procedure:

  • Add EMT Inducing Media Supplement directly to the standard culture media of your cells of interest
  • Culture cells for only 5 days to induce EMT
  • Evaluate EMT status using characteristic morphological changes and marker antibodies
 

 

Reagents Provided

Reagents supplied in the StemXVivo® EMT Inducing Media Supplement (Catalog # CCM017):

  • 1 mL of StemXVivo® EMT Inducing Media Supplement

Note: The quantity of media supplement is sufficient to drive EMT in the equivalent of fifteen 10 cm diameter tissue culture plates.

 

Other Supplies Required

Reagents

  • Cell culture medium
  • Dissociation solution (e.g., TrypLE™ Express, Invitrogen® or equivalent)
  • 0.4% Trypan blue solution

Materials

  • Epithelial cells of interest
  • Tissue culture plates/flasks
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 37 °C water bath
  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Harvest epithelial cells of interest using a dissociation solution.

<strong>Harvest</strong> epithelial cells of interest using a dissociation solution

Transfer the cell suspension to a 15 mL conical tube containing pre-warmed media.

Centrifuge at 400 x g for 5 minutes.

Transfer the cell suspension to a 15 mL conical tube containing pre-warmed media

Perform a cell count.

Perform a cell count

Plate cells at 0.9 - 1.0 x 104 cells/cm2 in media containing 1X EMT Inducing Media Supplement.

Plate cells at 0.9 - 1.0 x 10 4  cells/cm2 in media containing 1X EMT Inducing Media Supplement.
 

Culture cells and monitor cell morphology daily.

Replace media after 3 days.

Culture cells and monitor cell morphology daily

After 5 days, EMT induction is complete.

Evaluate EMT status using established markers and immunocytochemistry

Evaluate EMT status using established markers and immunocytochemistry

Citations (6)

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Customer Reviews (5)

4.4 out of 5
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  • StemXVivo EMT Inducing Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 02/20/2021
    StemXVivo EMT Inducing Media Supplement (100X) Serum CCM017
  • StemXVivo EMT Inducing Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 04/08/2019
    StemXVivo EMT Inducing Media Supplement (100X) Serum CCM017
  • StemXVivo EMT Inducing Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 04/04/2018
  • StemXVivo EMT Inducing Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 08/08/2017
    It seems very useful, but too expensive. I gave up using it because of the high cost.
  • StemXVivo EMT Inducing Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 04/12/2017
    StemXVivo EMT Inducing Media Supplement was used to induce EMT of ARPE-19 cells. Morphology and fibronectin measured by fibronectin ELISA (DY1918-05 and DY008, R & D Systems) are attached.
    StemXVivo EMT Inducing Media Supplement (100X) Serum CCM017

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FAQs

  • What is the composition of the medium used to culture MCF-7 cells for the data shown on the StemXVivo Media supplement (100X), Catalog # CCM017 product insert?

    The medium used to cuture MCF-7 cells for the data shown on Catalog # CCM017 is MEM containing NEAA, Sodium Pyruvate, 10% FBS, 2mM L-Glutamine, and Penicillin-Streptomycin

  • Has StemXVivo Media supplement (100X), Catalog # CCM017, been tested on mouse epithelial cells?

    Elaborate testing of Catlaog # CCM017 with mouse epithelial cells has not been performed. The supplement was tested once with NMuMG mouse mammary epithelial cells and found to induce EMT.

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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