Human Fibronectin Antibody

Catalog # MAB1918 | R&D Systems, Inc. a Bio-Techne Brand
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MAB1918
MAB1918-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P1H11

Product Summary for Human Fibronectin Antibody

Immunogen

Human plasma-derived Fibronectin

Specificity

Detects human Fibronectin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibronectin Antibody

Detection of Human Fibronectin by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Fibronectin antibody in HepG2 and Daudi Human Cell Lines by Immunocytochemistry (ICC).

Fibronectin in HepG2 and Daudi Human Cell Lines.

Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Fibronectin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Fibronectin in Human Breast Cancer Tissue.

Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and fibroblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications for Human Fibronectin Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine, kidney, and liver, and human breast cancer tissue

Immunoprecipitation

Yu, H. et al. (2004) Mol. Carcinog. 41:85.

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.4 using MAB1918 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

280794 (Bovine); 2335 (Human)

Gene Symbol

FN1

Product Documents for Human Fibronectin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Fibronectin Antibody

For research use only

Citations for Human Fibronectin Antibody (9)

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Citations are publications that use Bio-Techne products. Selected citations for Human Fibronectin Antibody include:

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Customer Reviews for Human Fibronectin Antibody (7)

4.4 out of 5
7 customer ratings
5 stars
43%
4 stars
57%
3 stars
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  • Human Fibronectin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Cardiac fibroblasts
    Species: Human
    Verified Customer | Posted 07/08/2022
    Human Fibronectin Antibody MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 09/28/2021
    Human Fibronectin Antibody MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 05/15/2020
    tested the antibody in whole cell ELISA using fibronectin overexpression cells. It works well.
    Human Fibronectin Antibody MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/01/2019
    Paired with AF1918
    Human Fibronectin Antibody MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 08/12/2019
    Human Fibronectin Antibody MAB1918
  • Name: cynthia morimoto
    Application: Western Blot
    Sample Tested: See PMID 22321604
    Species: Human
    Verified Customer | Posted 02/12/2015
  • Name: cynthia morimoto
    Application: ELISA
    Sample Tested: See PMID 22225866
    Species: Human
    Verified Customer | Posted 02/12/2015

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FAQs for Human Fibronectin Antibody

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  • Q: One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?

    A: Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

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