Human PDGF-AB DuoSet ELISA

Name: Human PDGF-AB DuoSet ELISA
Brand: R&D Systems
SKU: DY222
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY222

Citations (6)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	15.6 - 1,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human PDGF-AB DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human PDGF-AB DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PDGF-AB. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human PDGF-AB DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human PDGF-AB DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human PDGF-AB DuoSet ELISA

	Human PDGF-AB ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-AB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Item	Value
Long Name	Platelet-derived Growth Factor AB
Alternate Names	PDGF-AB, PDGFAB

Citations for Human PDGF-AB DuoSet ELISA (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-AB DuoSet ELISA include:

Species: Human
Sample Types: Plasma

A Jarosz et al. (2022), What Role Does PDGFA Gene Polymorphisms Play in Treating Tennis Elbow with PRP? A Prospective Cohort Study Oncogene, 11(12).
PMID: 35743573
Species: Human
Sample Types: Plasma

P Niemiec et al. (2021), Why PRP works only on certain patients with tennis elbow? Is PDGFB gene a key for PRP therapy effectiveness? A prospective cohort study Bmc Musculoskeletal Disorders, 22(1):710.
PMID: 34407802
Species: Human
Sample Types: Cell Culture Supernates

A Islam et al. (2019), Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells Eur Cell Mater, 37(0):153-174.
PMID: 30785213
Species: Human
Sample Types: Cell Culture Supernates

O Nebie et al. (2019), The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates J. Biomed. Sci., 26(1):89.
PMID: 31666073
Species: Human
Sample Types: Platelet Lysates

MS Chen et al. (2018), Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells New biotechnology, 0(0).
PMID: 30465908
Species: Human
Sample Types: Serum

Boneberg EM et al. (2009), Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer. Br. J. Cancer, 101(4):605-14.
PMID: 19672262

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Showing 1 - 6 of 6 citations Showing All

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