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Human IL-1 beta/IL-1F2 DuoSet ELISA

R&D Systems, Inc. a Bio-Techne Brand

Human IL-1 beta / IL-1F2 ELISA Standard Curve
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DY201
DY201-05

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Setting the standard in quality research reagents

Key Product Details

Assay Type: Solid Phase Sandwich ELISA
Assay Range: 3.91 - 250 pg/mL
Sample Types: Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human IL-1 beta/IL-1F2 DuoSet ELISA Features:
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Citations for Human IL-1 beta/IL-1F2 DuoSet ELISA (294)

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Customer Reviews for Human IL-1 beta/IL-1F2 DuoSet ELISA (30)

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30 customer ratings
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  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Supernates
    Verified Customer | Posted 02/13/2023
    Supernatants of HLF cells exposed to LPS for 6-hrs
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell Lysates
    Verified Customer | Posted 01/10/2023
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Peripheral blood mononuclear cells (PBMCs),plama
    Verified Customer | Posted 12/16/2022
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Supernates
    Verified Customer | Posted 06/20/2022
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Colon cancer tissue
    Verified Customer | Posted 09/29/2021
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Human cell conditioned medium
    Verified Customer | Posted 06/25/2021
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 05/12/2021
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 04/01/2021
    THP-1 cells were incubated with 0.1-10ug/ml PAM3CSK4 for 24-hours and culture supernatant was collected. IL-1b standard was fit to a sigmoidal 4-paramater logistic curve. Max IL-1b production was found at the 1ug/ml dosage.
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: krys bojanowski
    Sample Tested: Reconstituted Human Ciliated Airway
    Verified Customer | Posted 12/09/2020
    3D ciliated airway tissues (cat.# 502-3D-24) were from Cell Applications (San Diego, CA) and were cultured in maintenance medium (cat.# 511M-3D-100, Cell Applications). Twenty four hours after pre-incubation with test substances, tissues were exposed to 1µg/ml endotoxin LPS and IL-1b was was quantified after 48 additional hours. Worked very well.
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Robert Berahovich
    Sample Tested: Tissue Culture Media
    Verified Customer | Posted 09/29/2020
    Detection of IL-1b secreted by human CAR-T cells engineered to express IL-1b (PMC658), compared to normal CAR-T cells (PMC650).
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 08/11/2020
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: K562 human chronic myelogenous leukemia cell line
    Verified Customer | Posted 02/11/2020
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Evan Williams
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/15/2020
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: MICROGLIA CELLS
    Verified Customer | Posted 12/14/2019
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Microglia cells
    Verified Customer | Posted 12/14/2019
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Fiachra Humphries
    Sample Tested: human PBMC
    Verified Customer | Posted 09/11/2019
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Kim Berman
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 07/02/2019
    Not compatible with all substrates.
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Ivan Luzardo
    Sample Tested: Cell Lysates
    Verified Customer | Posted 01/03/2019
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 11/26/2018
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 10/07/2018
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Kim Han
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 07/03/2018
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 04/12/2018
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Leslie Priddy
    Sample Tested: Serum
    Verified Customer | Posted 03/30/2018
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: 7TD1 mouse hybridoma cell line,Human Whole Blood
    Verified Customer | Posted 02/27/2018
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Bone marrow-derived dendritic cells
    Verified Customer | Posted 02/06/2018
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 01/25/2018
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: U937 human histiocytic lymphoma cell line
    Verified Customer | Posted 10/17/2017
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: activated mouse CD8 T cell
    Verified Customer | Posted 09/08/2017
  • Human IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Human serum
    Verified Customer | Posted 04/03/2017
    Human IL-1 beta/IL-1F2 DuoSet ELISA DY201
  • Human IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate
    Name: Anonymous
    Sample Tested: THP-1 human acute monocytic leukemia cell line,Various primary cells
    Verified Customer | Posted 04/04/2016

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Product Documents for Human IL-1 beta/IL-1F2 DuoSet ELISA

Certificate of Analysis

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