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Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA

R&D Systems, Inc. a Bio-Techne Brand

This product has been discontinued
Human TGF-beta 1 ELISA Cell Culture Supernate/Urine Standard Curve
(3)
Human TGF-beta 1 ELISA Serum/Plasma Standard Curve
Human TGF-beta 1 ELISA Linearity
Discontinued Product
DB100B has been discontinued and is replaced by DB100C.
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Setting the standard in quality research reagents

Key Product Details

Assay Length: 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (100 uL), Serum (40 uL), EDTA Plasma (40 uL), Platelet-poor Plasma (25 uL), Urine (100 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Urine
Sensitivity: 15.4 pg/mL
Assay Range: 31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Urine)
Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Summary: The Quantikine Human TGF-beta 1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TGF-beta 1 in acid activated cell culture supernates, serum, plasma, and urine. It contains recombinant human TGF-beta 1 expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural TGF-beta 1 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural human TGF-beta 1.

Citations for Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA (391)

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Customer Reviews for Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA (20)

4.8 out of 5
20 customer ratings
5 stars
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  • Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 03/17/2022
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA
    Name: James Williams
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 03/16/2022
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media
    Verified Customer | Posted 01/10/2022
  • Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA
    Name: James AHODANTIN
    Sample Tested: Plasmas from mice fed with HFD
    Verified Customer | Posted 01/08/2022
    Very sensitive and easy to use. I switched to the new version DB100C lately and both are quite similar. Good product
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 09/01/2021
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media
    Verified Customer | Posted 07/02/2020
  • Human/Mouse/Rat/Porcine TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media
    Verified Customer | Posted 06/26/2020
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 02/28/2020
    Easy to use, great results
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Evan Williams
    Sample Tested: Plasma
    Verified Customer | Posted 02/27/2020
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Blood mononuclear cells (PBMCs)
    Verified Customer | Posted 01/16/2020
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Bone Extracts
    Verified Customer | Posted 10/10/2019
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 07/08/2019
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Blood mononuclear cells (PBMCs)
    Verified Customer | Posted 03/21/2019
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Ivan Luzardo
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 12/11/2018
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 06/19/2018
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Jim Hsiao
    Sample Tested: Mouse lung tissue homogenates,Mouse lung tissue
    Verified Customer | Posted 11/29/2017
    This result has been published in the PLoS ONE journal: https://doi.org/10.1371/journal.pone.0063798
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 07/21/2017
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 08/03/2016
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Muscle tissue
    Verified Customer | Posted 06/20/2016
    n/a
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B
  • Human TGF-beta 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Bone Putty
    Verified Customer | Posted 05/09/2016
    Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA DB100B

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FAQs for Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA

  • Can samples be tested without activation in Human TGF-beta 1 Quantikine® ELISA Kit, Catalog # DB100B, to determine levels of endogenously mature TGF-beta 1?

    Catalog # DB100B is designed to measure TGF-beta 1 in activated serum and plasma samples. Activation releases TGF-beta 1 from latent TGF-beta 1 complexes. Internal tests with latent TGF-beta complex have demonstrated approximately 15% reactivity in the kit; however it is unclear whether this may be partially due to activation of latent TGF-beta 1 once added to the assay plate. Since the latent form of TGF-beta 1 may be significantly more abundant in serum and plasma, this cross-reactivity may make it difficult to measure low levels of active TGF-beta 1. Therefore, we recommend only using the kit to test activated serum and plasma samples for total TGF-beta 1 levels.

Product Documents for Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.