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Human PlGF Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Length: | 3.5 hours or 4.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine |
| Sensitivity: | 7 pg/mL |
| Assay Range: | 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine) |
| Human PlGF Quantikine ELISA Kit Summary: | The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF. |
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Linearity
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Human PlGF ELISA Cell Culture Supernate Standard Curve |
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Human PlGF ELISA Serum/Plasma/Urine Standard Curve |
Citations for Human PlGF Quantikine ELISA Kit (73)
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Citations are publications that use Bio-Techne products. Selected citations for Human PlGF Quantikine ELISA Kit include:
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Species: Human
Sample Types: Serum
W Dymara-Kon et al. (2023-01-01), Similar Pro- and Antiangiogenic Profiles Close to Delivery in Different Clinical Presentations of Two Pregnancy Syndromes: Preeclampsia and Fetal Growth Restriction International Journal of Molecular Sciences, 24(2).
PMID: 36674486 -
Species: Human
Sample Types: Vitreous Humor
T Tsai et al. (2023-01-01), Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy PLoS ONE, 18(1):e0280488.
PMID: 36662891 -
Species: Human
Sample Types: Serum
H Wada et al. (2022-01-01), Impact of Chronic Kidney Disease on the Associations of Cardiovascular Biomarkers With Adverse Outcomes in Patients With Suspected or Known Coronary Artery Disease: The EXCEED-J Study Journal of the American Heart Association, 11(3):e023464.
PMID: 35048713 -
Species: Human
Sample Types: Plasma
EO Anto et al. (2022-01-01), Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia PLoS ONE, 17(3):e0265717.
PMID: 35312727 -
Species: Human
Sample Types: Vitreous Humor
I Klaassen et al. (2022-01-01), Vitreous protein networks around ANG2 and VEGF in proliferative diabetic retinopathy and the differential effects of aflibercept versus bevacizumab pre-treatment Scientific Reports, 12(1):21062.
PMID: 36473885 -
Species: Human
Sample Types: Plasma
S Kuriyama et al. (2022-01-01), Biomarkers for anti-vascular endothelial growth factor drugs Oncology Letters, 24(6):463.
PMID: 36406183 -
Species: Human
Sample Types: Serum
D Darmochwal et al. (2022-01-01), The Association of IL-17 and PlGF/sENG Ratio in Pre-Eclampsia and Adverse Pregnancy Outcomes International Journal of Environmental Research and Public Health, 20(1).
PMID: 36613090 -
Species: Human
Sample Types: Serum
DL Wilson et al. (2020-01-01), Sleep-disordered breathing does not impact maternal outcomes in women with hypertensive disorders of pregnancy PLoS ONE, 15(4):e0232287.
PMID: 32339208 -
Species: Human
Sample Types: Serum
P Nikuei et al. (2020-01-01), Diagnostic accuracy of sFlt1/PlGF ratio as a marker for preeclampsia BMC Pregnancy Childbirth, 20(1):80.
PMID: 32033594 -
Species: Mouse
Sample Types: Serum
JG Parchem et al. (2020-01-01), STOX1 deficiency is associated with renin-mediated gestational hypertension and placental defects JCI Insight, 0(0).
PMID: 33301424 -
Species: Human
Sample Types: Cell Culture Supernates
K Yagi et al. (2020-01-01), Potency of Tokishakuyakusan in treating preeclampsia: Drug repositioning method by in vitro screening of the Kampo library PLoS ONE, 15(12):e0244684.
PMID: 33378412 -
Species: Human
Sample Types: Serum
S Kim et al. (2020-01-01), Circulating miRNAs Associated with Dysregulated Vascular and Trophoblast Function as Target-Based Diagnostic Biomarkers for Preeclampsia Cells, 9(9).
PMID: 32878300 -
Species: Human
HW Unger et al. (2019-01-01), Sulphadoxine-pyrimethamine plus azithromycin may improve birth outcomes through impacts on inflammation and placental angiogenesis independent of malarial infection Sci Rep, 9(1):2260.
PMID: 30783215 -
Species: Human
Sample Types: Cell Culture Supernates
W Jiao et al. (2019-01-01), Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture Sci Rep, 9(1):15339.
PMID: 31653890 -
Species: Human
Sample Types: Cell Culture Supernates
KYB Lamm et al. (2018-01-01), Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome Elife, 7(0).
PMID: 29309034 -
Species: Human
Sample Types: Vitreous Humor
T Tsai et al. (2018-01-01), Anti-inflammatory cytokine and angiogenic factors levels in vitreous samples of diabetic retinopathy patients PLoS ONE, 13(3):e0194603.
PMID: 29584759 -
Species: Human
Sample Types: Serum
Y Nakada et al. (2018-01-01), Value of Placental Growth Factor as a Predictor of Adverse Events During the Acute Phase of Acute Decompensated Heart Failure Circ. J., 0(0).
PMID: 30587700 -
Species: Mouse
Sample Types: Tissue Culture Supernates
Applications: ELISA CaptureM Perrotta et al. (2018-01-01), Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation Cardiovasc. Res., 114(3):456-467.
PMID: 29324984 -
Species: Human
Sample Types: Serum
D Wu et al. (2017-01-01), Elevated expression of placental growth factor is associated with airway-wall vascular remodelling and thickening in smokers with asthma Sci Rep, 7(0):43017.
PMID: 28220848 -
Species: Human
Sample Types: Plasma
A Dutta et al. (2017-01-01), Household air pollution and angiogenic factors in pregnant Nigerian women: A randomized controlled ethanol cookstove intervention Sci. Total Environ., 599(0):2175-2181.
PMID: 28575932 -
Species: Human
Sample Types: Tissue Culture Supernates
M O'Brien et al. (2017-01-01), Endothelial Dysfunction in Severe Preeclampsia is Mediated by Soluble Factors, Rather than Extracellular Vesicles Sci Rep, 7(1):5887.
PMID: 28725005 -
Species: Human
Sample Types: Serum
X Li et al. (2017-01-01), Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease J. Cell. Mol. Med., 0(0).
PMID: 28378526 -
Species: Human
Sample Types: Cell Culture Supernates
D Dauwe et al. (2016-01-01), Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved J Am Heart Assoc, 5(4).
PMID: 27091182 -
Species: Human
Sample Types: Serum
VS Jammalamad et al. (2016-01-01), Spectrum of Factors Triggering Endothelial Dysfunction in PIH J Clin Diagn Res, 10(12):BC14-BC17.
PMID: 28208844 -
Species: Human
Sample Types: Tissue Homogenates
Ingrid C Weel (2016-01-01), Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia PLoS ONE, 11(6):e0157584.
PMID: 27315098 -
Species: Human
Sample Types: Serum
S Senapati et al. (2016-01-01), Predicting first trimester pregnancy outcome: derivation of a multiple marker test Fertil. Steril., 106(7):1725-1732.e3.
PMID: 28340932 -
Species: Human
Sample Types: Cell Culture Supernates
Bessho H et al. (2015-01-01), Inhibition of placental growth factor in renal cell carcinoma. Anticancer Res, 35(1):531-41.
PMID: 25550599 -
Species: Human
Sample Types: Serum
White W et al. (2014-01-01), Persistent urinary podocyte loss following preeclampsia may reflect subclinical renal injury. PLoS ONE, 9(3):e92693.
PMID: 24664365 -
Species: Human
Sample Types: Cell Culture Supernates
Hou H et al. (2014-01-01), Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis. Autophagy, 10(9):1509-21.
PMID: 24988221 -
Species: Human
Sample Types: Plasma
Calcaterra F et al. (2014-01-01), Reduction of maternal circulating endothelial progenitor cells in human pregnancies with intrauterine growth restriction. Placenta, 35(7):431-6.
PMID: 24819155 -
Species: Human
Sample Types: Serum
El-Tarhouny S et al. (2014-01-01), Placental growth factor and soluble Fms-like tyrosine kinase 1 in diabetic pregnancy: A possible relation to distal villous immaturity. Histol Histopathol, 29(2):259-72.
PMID: 23939615 -
Species: Human
Sample Types: Cell Culture Supernates
Chaturvedi et al. (2013-01-01), Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis. J Clin Invest, 123(1):189-205.
PMID: 23318994 -
Species: Human
Sample Types: Serum
Liu et al. (2013-01-01), Integrating multiple 'omics' analyses identifies serological protein biomarkers for preeclampsia. BMC Med, 11(0):236.
PMID: 24195779 -
Species: Human
Sample Types: Cell Culture Supernates
Huang Q et al. (2013-01-01), Advanced oxidation protein products enhances soluble Fms-like tyrosine kinase 1 expression in trophoblasts: a possible link between oxidative stress and preeclampsia. Placenta, 34(10):949-52.
PMID: 23899470 -
Species: Human
Sample Types: Tissue Homogenates
Yamashita-Kashima Y et al. (2012-01-01), Biomarkers for antitumor activity of bevacizumab in gastric cancer models. BMC Cancer, 12(0):37.
PMID: 22273502 -
Species: Human
Sample Types: Serum
(2012-01-01), Cytokine and angiogenic factors associated with efficacy and toxicity of pazopanib in advanced soft-tissue sarcoma: an EORTC-STBSG study. Br. J. Cancer, 107(4):639-45.
PMID: 22805326 -
Species: Human
Sample Types: Plasma
Loupakis F et al. (2011-01-01), Pharmacodynamic and pharmacogenetic angiogenesis-related markers of first-line FOLFOXIRI plus bevacizumab schedule in metastatic colorectal cancer. Br. J. Cancer, 104(8):1262-9.
PMID: 21407216 -
Species: Human
Sample Types: Serum
Rahbari NN et al. (2011-01-01), Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer. BMC Cancer, 11(0):286.
PMID: 21729304 -
Species: Human
Sample Types: Plasma
Dubois SG et al. (2011-01-01), Phase I and pharmacokinetic study of sunitinib in pediatric patients with refractory solid tumors: a children's oncology group study. Clin. Cancer Res., 17(15):5113-22.
PMID: 21690570 -
Species: Human
Sample Types: Plasma
de Groot JF et al. (2011-01-01), Myeloid Biomarkers Associated with Glioblastoma Response to Anti-VEGF Therapy with Aflibercept. Clin. Cancer Res., 17(14):4872-4881.
PMID: 21632852 -
Species: Human
Sample Types: Serum
Verlohren S et al. (2010-01-01), An automated method for the determination of the sFlt-1/PIGF ratio in the assessment of preeclampsia. Am. J. Obstet. Gynecol., 202(2):161.e1-161.e1.
PMID: 19850276 -
Species: Human
Sample Types: Serum
Powers RW et al. (2010-01-01), Soluble fms-Like tyrosine kinase 1 (sFlt1), endoglin and placental growth factor (PlGF) in preeclampsia among high risk pregnancies. PLoS ONE, 5(10):e13263.
PMID: 20948996 -
Species: Human
Sample Types: Serum
Horne AW et al. (2010-01-01), Placental Growth Factor: A Promising Diagnostic Biomarker for Tubal Ectopic Pregnancy. J. Clin. Endocrinol. Metab., 0(0).
PMID: 21047920 -
Species: Human
Sample Types: Tissue Homogenates
Jaffe IZ et al. (2010-01-01), Placental growth factor mediates aldosterone-dependent vascular injury in mice. J. Clin. Invest., 120(11):3891-900.
PMID: 20921624 -
Species: Human
Sample Types: Serum
Jeyabalan A et al. (2010-01-01), Effect of smoking on circulating angiogenic factors in high risk pregnancies. PLoS ONE, 5(10):e13270.
PMID: 20967275 -
Species: Human
Sample Types: Cell Culture Supernates
Brave SR et al. (2010-01-01), Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. Angiogenesis, 13(4):337-47.
PMID: 20953695 -
Species: Human
Sample Types: Cell Culture Supernates
Ha CT et al. (2010-01-01), Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis. Biol. Reprod., 83(1):27-35.
PMID: 20335639 -
Species: Human
Sample Types: Plasma
Brittain JE et al. (2010-01-01), Placenta growth factor in sickle cell disease: association with hemolysis and inflammation. Blood, 115(10):2014-20.
PMID: 20040765 -
Species: Human
Sample Types: Plasma
Cassidy A et al. (2009-01-01), Potential role for plasma placental growth factor in predicting coronary heart disease risk in women. Arterioscler. Thromb. Vasc. Biol., 29(1):134-9.
PMID: 18927470 -
Species: Human
Sample Types: Cell Culture Supernates
Manaster I et al. (2008-01-01), Endometrial NK cells are special immature cells that await pregnancy. J. Immunol., 181(3):1869-76.
PMID: 18641324 -
Species: Human
Sample Types: Plasma
Jeyabalan A et al. (2008-01-01), Cigarette smoke exposure and angiogenic factors in pregnancy and preeclampsia. Am. J. Hypertens., 21(8):943-7.
PMID: 18566591 -
Species: Human
Sample Types: Plasma
Rini BI et al. (2008-01-01), Antitumor activity and biomarker analysis of sunitinib in patients with bevacizumab-refractory metastatic renal cell carcinoma. J. Clin. Oncol., 26(22):3743-8.
PMID: 18669461 -
Species: Human
Sample Types: Plasma
Findley CM et al. (2008-01-01), Plasma levels of soluble Tie2 and vascular endothelial growth factor distinguish critical limb ischemia from intermittent claudication in patients with peripheral arterial disease. J. Am. Coll. Cardiol., 52(5):387-93.
PMID: 18652948 -
Species: Human
Sample Types: Cell Culture Supernates
Sorrentino A et al. (2008-01-01), Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 36(8):1035-46.
PMID: 18504067 -
Species: Human
Sample Types: Serum
Gutman G et al. (2008-01-01), Regulation of vascular endothelial growth factor-A and its soluble receptor sFlt-1 by luteinizing hormone in vivo: implication for ovarian follicle angiogenesis. Fertil. Steril., 89(4):922-6.
PMID: 18343373 -
Species: Human
Sample Types: Serum
Wallner W et al. (2007-01-01), Angiogenic growth factors in maternal and fetal serum in pregnancies complicated by intrauterine growth restriction. Clin. Sci., 112(1):51-7.
PMID: 16928195 -
Species: Human
Sample Types: Bone Marrow Fluid
Kissel CK et al. (2007-01-01), Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure. J. Am. Coll. Cardiol., 49(24):2341-9.
PMID: 17572250 -
Species: Human
Sample Types: Plasma
Apple FS et al. (2007-01-01), Multiple biomarker use for detection of adverse events in patients presenting with symptoms suggestive of acute coronary syndrome. Clin. Chem., 53(5):874-81.
PMID: 17384009 -
Species: Human
Sample Types: Serum
Levine RJ et al. (2006-01-01), Soluble endoglin and other circulating antiangiogenic factors in preeclampsia. N. Engl. J. Med., 355(10):992-1005.
PMID: 16957146 -
Species: Human
Sample Types: Plasma
Iwama H et al. (2006-01-01), Cardiac expression of placental growth factor predicts the improvement of chronic phase left ventricular function in patients with acute myocardial infarction. J. Am. Coll. Cardiol., 47(8):1559-67.
PMID: 16630991 -
Species: Human
Sample Types: Cell Culture Supernates
Moreno MJ et al. (2006-01-01), Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells. Glia, 53(8):845-57.
PMID: 16586492 -
Species: Human
Sample Types: Serum
Masuyama H et al. (2006-01-01), Circulating angiogenic factors in preeclampsia, gestational proteinuria, and preeclampsia superimposed on chronic glomerulonephritis. Am. J. Obstet. Gynecol., 194(2):551-6.
PMID: 16458660 -
Species: Human
Sample Types: Tissue Homogenates
Cianfarani F et al. (2006-01-01), Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am. J. Pathol., 169(4):1167-82.
PMID: 17003476 -
Species: Human
Sample Types: Urine
Buhimschi CS et al. (2005-01-01), Urinary angiogenic factors cluster hypertensive disorders and identify women with severe preeclampsia. Am. J. Obstet. Gynecol., 192(3):734-41.
PMID: 15746665 -
Species: Human
Sample Types: Serum
Briguori C et al. (2005-01-01), Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals. Am. J. Cardiol., 97(4):472-6.
PMID: 16461039 -
Species: Human
Sample Types: Urine
Levine RJ et al. (2005-01-01), Urinary placental growth factor and risk of preeclampsia. JAMA, 293(1):77-85.
PMID: 15632339 -
Species: Human
Sample Types: Serum
Levine RJ et al. (2004-01-01), Circulating angiogenic factors and the risk of preeclampsia. N. Engl. J. Med., 350(7):672-83.
PMID: 14764923 -
Species: Human
Sample Types: Cell Lysates
Nishishita T et al. (2004-01-01), A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells. Biochem. Biophys. Res. Commun., 325(1):24-31.
PMID: 15522196 -
Species: Human
Sample Types: Serum
Maynard SE et al. (2003-01-01), Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J. Clin. Invest., 111(5):649-58.
PMID: 12618519 -
Species: Human
Sample Types: Serum
Tsao PN et al. (2003-01-01), Overexpression of placenta growth factor contributes to the pathogenesis of pulmonary emphysema. Am. J. Respir. Crit. Care Med., 169(4):505-11.
PMID: 14644931 -
Species: Human
Sample Types: Cell Culture Supernates
Zhou Y et al. (2002-01-01), Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. , 160(4):1405-23.
PMID: 11943725 -
Sample Types: Serum
Chappell LC et al. (2002-01-01), A longitudinal study of biochemical variables in women at risk of preeclampsia. Am. J. Obstet. Gynecol., 187(1):127-36.
PMID: 12114900 -
Species: Human
Sample Types: Cell Lysates
Oura H et al. (2002-01-01), A critical role of placental growth factor in the induction of inflammation and edema formation. Blood, 101(2):560-7.
PMID: 12393422
There are no citations that match your criteria.
Customer Reviews for Human PlGF Quantikine ELISA Kit (3)
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Sample Tested: Cell culture supernatantVerified Customer | Posted 05/20/2023this is a reliable ELISA kit for PLGF in a variety of sample types. unlike some of the other quantikine kits this protocol requires 100 uL of sample however.
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Sample Tested: Human fibroblastVerified Customer | Posted 10/11/2022Human PIGF was evaluated in HUVEC cells extracted from fetal membranes and exposed to pro-inflammatory factors (IL-1b and TNF-a). A significant increase in PIGIF production was obtained, compared to the untreated cells.
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Sample Tested: Human fetal membranesVerified Customer | Posted 09/23/2021Human PIGF levels were evaluated in fetal membrane explants extracted from women voluntarily subjected to the caesarian procedure. Membranes were seeded in transwell inserts for 7 days, and PIGF levels were quantified. Since PIGF is essential for fetal development and health, quantification obtained from this kit was critical to assess its levels. Overall, the kit is straightforward to follow, and the instructions are very clear. The picture shows the PIGF levels after maintaing the fetal membranes for 4 and 8 h, without treatment (control) and treated with TNF-a
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FAQs for Human PlGF Quantikine ELISA Kit
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Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?
Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.
Product Documents for Human PlGF Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
