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Human PlGF Quantikine ELISA Kit

R&D Systems, Inc. a Bio-Techne Brand

Human PlGF ELISA Cell Culture Supernate Standard Curve
(3)
Human PlGF ELISA Serum/Plasma/Urine Standard Curve
Human PlGF ELISA Linearity
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PDPG00
DPG00
SPG00

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Setting the standard in quality research reagents

Key Product Details

Assay Length: 3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Sensitivity: 7 pg/mL
Assay Range: 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Human PlGF Quantikine ELISA Kit Summary: The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Citations for Human PlGF Quantikine ELISA Kit (73)

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Customer Reviews for Human PlGF Quantikine ELISA Kit (3)

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  • Human PlGF Quantikine ELISA Kit
    Name: Vincent Giacalone
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 05/20/2023
    this is a reliable ELISA kit for PLGF in a variety of sample types. unlike some of the other quantikine kits this protocol requires 100 uL of sample however.
    Human PlGF Quantikine ELISA Kit DPG00
  • Human PlGF Quantikine ELISA Kit
    Name: Andres Ocampo
    Sample Tested: Human fibroblast
    Verified Customer | Posted 10/11/2022
    Human PIGF was evaluated in HUVEC cells extracted from fetal membranes and exposed to pro-inflammatory factors (IL-1b and TNF-a). A significant increase in PIGIF production was obtained, compared to the untreated cells.
    Human PlGF Quantikine ELISA Kit DPG00
  • Human PlGF Quantikine ELISA Kit
    Name: Martin Torres
    Sample Tested: Human fetal membranes
    Verified Customer | Posted 09/23/2021
    Human PIGF levels were evaluated in fetal membrane explants extracted from women voluntarily subjected to the caesarian procedure. Membranes were seeded in transwell inserts for 7 days, and PIGF levels were quantified. Since PIGF is essential for fetal development and health, quantification obtained from this kit was critical to assess its levels. Overall, the kit is straightforward to follow, and the instructions are very clear. The picture shows the PIGF levels after maintaing the fetal membranes for 4 and 8 h, without treatment (control) and treated with TNF-a
    Human PlGF Quantikine ELISA Kit DPG00

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FAQs for Human PlGF Quantikine ELISA Kit

  • Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

    Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

Product Documents for Human PlGF Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.