Human GM-CSF Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 3.5 hours or 4.5 hours |
Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL) |
Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma |
Sensitivity: | 3 pg/mL |
Assay Range: | 7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma) |
Human GM-CSF Quantikine ELISA Kit Summary: | The Quantikine Human GM-CSF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure GM-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human GM-CSF accurately. Results obtained using natural human GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GM-CSF. |
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Citations for Human GM-CSF Quantikine ELISA Kit (55)
Citations are publications that use Bio-Techne products. Selected citations for Human GM-CSF Quantikine ELISA Kit include:
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Species: Mouse
Sample Types: Milk
AV Smirnov et al. (2022-01-01), Evaluation of the alpha-casein (CSN1S1) locus as a potential target for a site-specific transgene integration Scientific Reports, 12(1):7983.
PMID: 35568783 -
Species: Human
Sample Types: Serum
C Papagoras et al. (2022-01-01), Serum granulocyte-macrophage colony-stimulating factor (GM-CSF) is increased in patients with active radiographic axial spondyloarthritis and persists despite anti-TNF treatment Arthritis Research Therapy, 2022;24(1):195.
PMID: 35974380 -
Species: Human
Sample Types: Cell Culture Supernates
N Jirapongwa et al. (2022-01-01), Mesothelin?specific T cell cytotoxicity against triple negative breast cancer is enhanced by 40s ribosomal protein subunit 3?treated self?differentiated dendritic cells Oncology reports, 48(1).
PMID: 35616135 -
Species: Human
Sample Types: Cell Culture Supernates
F Zhou et al. (2021-01-01), miR-31 attenuates murine allergic rhinitis by suppressing interleukin-13-induced nasal epithelial inflammatory responses Mol Med Rep, 23(1).
PMID: 33179116 -
Species: Human
Sample Types: Cell Culture Supernates
W Gao et al. (2021-01-01), Psoralen inhibits the inflammatory response and mucus production in allergic rhinitis by inhibiting the activator protein�1 pathway and the downstream expression of cystatin?SN Molecular Medicine Reports, 24(3).
PMID: 34278468 -
Species: Human
Sample Types: Cell Culture Supernates
S Zhang et al. (2021-01-01), miR-124-3p relieves allergic rhinitis by inhibiting dipeptidyl peptidase-4 International immunopharmacology, 101(0):108279.
PMID: 34715574 -
Species: Human
Sample Types: Cell Culture Supernates
B Cao et al. (2021-01-01), Development of mesothelin-specific CAR NK-92 cells for the treatment of gastric cancer International journal of biological sciences, 17(14):3850-3861.
PMID: 34671203 -
Species: Human
Sample Types: Cell Culture Supernates
DH Kim et al. (2020-01-01), Suppressive effects of S100A8 and S100A9 on neutrophil apoptosis by cytokine release of human bronchial epithelial cells in asthma Int J Med Sci, 17(4):498-509.
PMID: 32174780 -
Species: Human
Sample Types: Cell Culture Supernates
M Drill et al. (2020-01-01), Inhibition of purinergic P2X receptor 7 (P2X7R) decreases granulocyte-macrophage colony-stimulating factor (GM-CSF) expression in U251 glioblastoma cells Sci Rep, 10(1):14844.
PMID: 32908225 -
Species: Human
Sample Types: Cell Culture Supernates
D Hao et al. (2019-01-01), Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy Cell Death Dis, 10(1):19.
PMID: 30622245 -
Species: Human
Sample Types: Cell Culture Supernates
T Sun et al. (2019-01-01), Multilevel defects in the hematopoietic niche in essential thrombocythemia Haematologica, 0(0).
PMID: 31289202 -
Sample Types: Cell Culture Supernates
V Kemp et al. (2018-01-01), Arming oncolytic reovirus with GM-CSF gene to enhance immunity Cancer Gene Ther., 0(0).
PMID: 30467340 -
Species: Human
Sample Types: Cell Culture Supernates
K Huang et al. (2018-01-01), Glucocorticoid insensitivity by staphylococcal enterotoxin B in keratinocytes of allergic dermatitis is associated with impaired nuclear translocation of the Glucocorticoid Receptor ? J. Dermatol. Sci., 92(3):272-280.
PMID: 30522881 -
Species: Human
Sample Types: Serum
Applications: ELISA CaptureG Jordakieva et al. (2018-01-01), Occupational exposure to ultrafine particles in police officers: no evidence for adverse respiratory effects J Occup Med Toxicol, 13(0):5.
PMID: 29434649 -
Species: Human
Sample Types: Cell Culture Supernates
F Reggiani et al. (2017-01-01), Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression Cancer Res., 0(0).
PMID: 28754674 -
YH Sun et al. (2017-01-01), Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1 Mol Med Rep, 0(0).
PMID: 28560433 -
Species: Human
Sample Types: Tissue Homogenates
Ingrid C Weel (2016-01-01), Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia PLoS ONE, 11(6):e0157584.
PMID: 27315098 -
Species: Human
Sample Types: Cell Culture Supernates
Pundir P et al. (2015-01-01), The Novel Receptor C5aR2 Is Required for C5a-Mediated Human Mast Cell Adhesion, Migration, and Proinflammatory Mediator Production. J Immunol, 195(6):2774-87.
PMID: 26283482 -
Species: Human
Sample Types: Plasma
Park S et al. (2015-01-01), Phase 1b Trial of Biweekly Intravenous Pexa-Vec (JX-594), an Oncolytic and Immunotherapeutic Vaccinia Virus in Colorectal Cancer. Mol Ther, 23(9):1532-40.
PMID: 26073886 -
Species: Human
Sample Types: Cell Culture Supernates
Spampinato S et al. (2015-01-01), Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli? PLoS ONE, 10(7):e0133392.
PMID: 26197437 -
Species: Human
Sample Types: Serum
Dickinson R et al. (2014-01-01), The evolution of cellular deficiency in GATA2 mutation. Blood, 123(6):863-74.
PMID: 24345756 -
Species: Human
Sample Types: Cell Culture Supernates
Huang et al. (2014-01-01), Autophagy promotes radiation-induced senescence but inhibits bystander effects in human breast cancer cells. Autophagy, 10(7):1212-28.
PMID: 24813621 -
Species: Human
Sample Types: Cell Culture Supernates
Smrz D et al. (2013-01-01), Prevention of F-actin assembly switches the response to SCF from chemotaxis to degranulation in human mast cells. Eur J Immunol, 43(7):1873-82.
PMID: 23616175 -
Species: Human
Sample Types: Cell Culture Supernates
Sawada H et al. (2013-01-01), Reduced BMPR2 expression induces GM-CSF translation and macrophage recruitment in humans and mice to exacerbate pulmonary hypertension. J Exp Med, 211(2):263-80.
PMID: 24446489 -
Species: Human
Sample Types: Cell Culture Supernates
Houser KR et al. (2012-01-01), Anti-inflammatory effects of methoxyphenolic compounds on human airway cells. J Inflamm (Lond), 9(0):6.
PMID: 22414048 -
Species: Human
Sample Types: Cell Culture Supernates
Freund A et al. (2011-01-01), p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype. EMBO J., 30(8):1536-48.
PMID: 21399611 -
Species: Human
Sample Types: Cell Culture Supernates
Beziat V et al. (2010-01-01), Shaping of iNKT cell repertoire after unrelated cord blood transplantation. Clin. Immunol., 135(3):364-73.
PMID: 20153980 -
Species: Human
Sample Types: Tissue Homogenates
Huang SJ et al. (2010-01-01), The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia. Am. J. Pathol., 177(5):2472-82.
PMID: 20829438 -
Species: Human
Sample Types: Plasma
Calmy A et al. (2009-01-01), HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial. AIDS, 23(8):929-39.
PMID: 19425222 -
Species: Human
Sample Types: Cell Culture Supernates
Schuster C et al. (2009-01-01), HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 206(1):169-81.
PMID: 19139172 -
Species: Rabbit
Sample Types: Vaginal Lavage Fluid
Aranha CC et al. (2008-01-01), Assessment of cervicovaginal cytokine levels following exposure to microbicide Nisin gel in rabbits. Cytokine, 43(1):63-70.
PMID: 18513989 -
Species: Human
Sample Types: BALF
Hartl D et al. (2008-01-01), Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 181(11):8053-67.
PMID: 19017998 -
Species: Human
Sample Types: Cell Culture Supernates
Sorrentino A et al. (2008-01-01), Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 36(8):1035-46.
PMID: 18504067 -
Species: Human
Sample Types: Serum
Makowska JS et al. (2007-01-01), Systemic responses after bronchial aspirin challenge in sensitive patients with asthma. J. Allergy Clin. Immunol., 121(2):348-54.
PMID: 18086495 -
Species: Human
Sample Types: Serum
Vassina EM et al. (2006-01-01), cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis. Eur. J. Immunol., 36(7):1975-84.
PMID: 16761316 -
Species: Human
Sample Types: Cell Culture Supernates
Pang L et al. (2006-01-01), Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities. J. Immunol., 176(6):3788-95.
PMID: 16517749 -
Species: Human
Sample Types: Cell Culture Supernates
Hatfield K et al. (2006-01-01), Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Int. J. Cancer, 119(10):2313-21.
PMID: 16921487 -
Species: Human
Sample Types: Cell Culture Supernates
Anderson ST et al. (2006-01-01), Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing. Am. J. Respir. Crit. Care Med., 173(9):1038-42.
PMID: 16456140 -
Species: Human
Sample Types: Whole Cells
Ameredes BT et al. (2005-01-01), Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle. J. Allergy Clin. Immunol., 116(1):65-72.
PMID: 15990776 -
Species: Human
Sample Types: Cell Culture Supernates
Bartemes KR et al. (2005-01-01), Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions. J. Allergy Clin. Immunol., 116(4):827-35.
PMID: 16210057 -
Species: Human
Sample Types: BALF
Chen LL et al. (2004-01-01), Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects. Chest, 125(6):2328-35.
PMID: 15189958 -
Species: Human
Sample Types: Cell Culture Supernates
Kahlon KS et al. (2004-01-01), Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells. Cancer Res., 64(24):9160-6.
PMID: 15604287 -
Species: Human
Sample Types: CSF
Henkel JS et al. (2004-01-01), Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue. Ann. Neurol., 55(2):221-35.
PMID: 14755726 -
Species: Human
Sample Types: Serum
Xiao T et al. (2003-01-01), Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma. Cytokine, 23(4):126-32.
PMID: 12967648 -
Species: Human
Sample Types: Serum
Lamming CE et al. (2003-01-01), Spontaneous circulation of myeloid-lymphoid-initiating cells and SCID-repopulating cells in sickle cell crisis. 111(6):811-9.
PMID: 12639987 -
Species: Human
Sample Types: Peritoneal Fluid
Hellman C et al. (2003-01-01), Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Clin. Exp. Immunol., 131(1):75-81.
PMID: 12519389 -
Species: Human
Sample Types: Cell Culture Supernates
Qu C et al. (2003-01-01), Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC. J. Immunol., 170(2):1010-8.
PMID: 12517968 -
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Cell Culture Supernates
de Revel T et al. (2002-01-01), Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation. Br. J. Haematol., 118(3):875-84.
PMID: 12181061 -
Species: Human
Sample Types: Urine
Pages F et al. (2002-01-01), Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer. Clin. Exp. Immunol., 127(2):303-9.
PMID: 11876754 -
Species: Human
Sample Types: Cell Culture Supernates
Loeuillet C et al. (2001-01-01), Distinct hematopoietic support by two human stromal cell lines. Exp. Hematol., 29(6):736-45.
PMID: 11378269 -
Species: Human
Sample Types: Cell Culture Supernates
Kadowaki N et al. (2000-01-01), Natural interferon alpha/beta-producing cells link innate and adaptive immunity. J. Exp. Med., 192(2):219-26.
PMID: 10899908 -
Species: Human
Sample Types: Cell Culture Supernates
Szabowski A et al. (2000-01-01), c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell, 103(5):745-55.
PMID: 11114331 -
Species: Human
Sample Types: Cell Culture Supernates
Sato E et al. (2000-01-01), Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity. Eur. Respir. J., 16(5):951-8.
PMID: 11153598 -
Species: Human
Sample Types: Serum
Chopra V et al. (1997-01-01), Serum levels of interleukins, growth factors and angiogenin in patients with endometrial cancer. J. Cancer Res. Clin. Oncol., 123(3):167-72.
PMID: 9119882 -
Species: Human
Sample Types: Cell Culture Supernates
Rudisch A et al. (), High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts. PLoS ON10(4):e0124283.
PMID: 25919140
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Customer Reviews for Human GM-CSF Quantikine ELISA Kit (4)
Customer Images
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Sample Tested: SerumVerified Customer | Posted 04/05/2022Clear instructions and robust method
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Sample Tested: PANC-1 human pancreatic carcinoma cell lineVerified Customer | Posted 05/11/2018
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Sample Tested: Serum and PlasmaVerified Customer | Posted 04/04/2018
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Sample Tested: SerumVerified Customer | Posted 04/24/2017The Human GM-CSF Quantikine ELISA kit is used for diagnostic use.
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Product Documents for Human GM-CSF Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.