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Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Catalog # QLB00B
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Key Product Details
| Assay Length: | 5.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma |
| Sensitivity: | 0.55 pg/mL |
| Assay Range: | 1.4 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma) |
| Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit Summary: | The QuantiGlo Human IL-1 beta Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant protein. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids. |
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Human IL-1 beta/IL-1F2 Chemiluminescent ELISA Standard Curve |
Citations for Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit (11)
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Citations are publications that use Bio-Techne products. Selected citations for Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit include:
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Species: Human
Sample Types: Cell Culture Supernates
C Ge et al. (2022), Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK Oncogene, 13(1):5945.
PMID: 36209205 -
Species: Human
Sample Types: Cell Culture Supernates
DQ Song et al. (2021), Procyanidin�B2 inhibits lipopolysaccharide?induced apoptosis by suppressing the Bcl?2/Bax and NF?&kappaB signalling pathways in human umbilical vein endothelial cells Molecular Medicine Reports, 23(4):1.
PMID: 33576443 -
Species: Human
Sample Types: Cell Culture Supernates
X Shao et al. (2020), Polarized Cytokine Release Triggered by P2X7 Receptor from Retinal Pigmented Epithelial Cells Dependent on Calcium Influx Cells, 9(12).
PMID: 33255431 -
Species: Human
Sample Types: cell culture
S Yuan et al. (2019), miR-98 Modulates Cytokine Production from Human PBMCs in Systemic Lupus Erythematosus by Targeting IL-6 mRNA J Immunol Res, 2019(0):9827574.
PMID: 31886314 -
Species: Human
Sample Types: Whole Tissue
M Egawa et al. (2018), Evaluation of psychological stress in confined environments using salivary, skin, and facial image parameters Sci Rep, 8(1):8264.
PMID: 29844534 -
Species: Human
Sample Types: Cell Culture Supernates
A Abrahamsso et al. (2017), Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women Oncotarget, 8(54):92134-92142.
PMID: 29190903 -
Species: Human
Sample Types: Cell Culture Supernates
Cifuentes M et al. (2012), Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue. Mol Cell Endocrinol, 361(1):24-30.
PMID: 22449852 -
Species: Human
Sample Types: Reference Standard
Fichorova RN et al. (2008), Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study. Anal. Chem., 80(12):4741-51.
PMID: 18484740 -
Species: Human
Sample Types: Cell Lysates
Wilson HL et al. (2007), P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br. J. Pharmacol., 151(1):115-27.
PMID: 17351655 -
Species: Human
Sample Types: Plasma
Dahaba AA et al. (2002), Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients. Anaesth Intensive Care, 30(3):269-74.
PMID: 12075632 -
Species: Human
Sample Types: Cell Culture Supernates
MacKenzie A et al. (2001), Rapid secretion of interleukin-1beta by microvesicle shedding. Immunity, 15(5):825-35.
PMID: 11728343
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