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Endothelin-1 Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Length: | 4.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (75 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Urine (75 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine |
| Sensitivity: | 0.207 pg/mL |
| Assay Range: | 0.39 - 25 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine) |
| Endothelin-1 Quantikine ELISA Kit Summary: | The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1. |
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Multi-species Endothelin-1 ELISA Standard Curve |
Citations for Endothelin-1 Quantikine ELISA Kit (74)
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Citations are publications that use Bio-Techne products. Selected citations for Endothelin-1 Quantikine ELISA Kit include:
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Species: Human
Sample Types: Serum
V Pulito-Cue et al. (2023-01-01), Endothelin-1 as a Biomarker of Idiopathic Pulmonary Fibrosis and Interstitial Lung Disease Associated with Autoimmune Diseases International Journal of Molecular Sciences, 24(2).
PMID: 36674789 -
Species: Human
Sample Types: Plasma
X Yang et al. (2022-01-01), LncRNA MALAT1 functions as a biomarker of no-reflow phenomenon in ST-segment elevation myocardial infarction patients receiving primary percutaneous coronary intervention Scientific Reports, 12(1):3294.
PMID: 35228564 -
Species: Mouse
Sample Types: Plasma
CT Vong et al. (2022-01-01), Classical prescription Dachuanxiong Formula delays nitroglycerin-induced pain response in migraine mice through reducing endothelin-1 level and regulating fatty acid biosynthesis Journal of ethnopharmacology, 0(0):114992.
PMID: 35032586 -
Species: Human
Sample Types: Serum
V Montiel et al. (2022-01-01), Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients EBioMedicine, 77(0):103893.
PMID: 35219085 -
Species: Human
Sample Types: Serum
S Macdonald et al. (2022-01-01), No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department Scientific Reports, 12(1):8733.
PMID: 35610344 -
Species: Human
Sample Types: Aqueous Humor
HM Kang et al. (2022-01-01), Elevated aqueous endothelin-1 concentrations in advanced diabetic retinopathy PLoS ONE, 17(5):e0268353.
PMID: 35544533 -
Species: Human
Sample Types: Serum
BR Fato et al. (2022-01-01), Serum Collected from Preeclamptic Pregnancies Drives Vasoconstriction of Human Omental Arteries-A Novel Ex Vivo Model of Preeclampsia for Therapeutic Development International Journal of Molecular Sciences, 23(18).
PMID: 36142764 -
Species: Human
Sample Types: Serum
J Baran et al. (2022-01-01), Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes Oncogene, 9(6).
PMID: 35735822 -
Species: Human
Sample Types: Plasma
NP Rocha et al. (2022-01-01), Cerebral blood flow is associated with markers of neurodegeneration in Huntington's disease Parkinsonism related disorders, 2022;102(0):79-85.
PMID: 35973322 -
Species: Human
Sample Types: Serum
E Tudorache et al. (2022-01-01), Endothelial dysfunction: The possible link between cardiovascular comorbidities and phenomenon of inflammaging from COPD Medicine, 101(33):e30078.
PMID: 35984178 -
Species: Human
Sample Types: Serum
NG Gumanova et al. (2022-01-01), Serum biomarkers, including nitric oxide metabolites (NOx), for prognosis of cardiovascular death and acute myocardial infarction in an ESSE-RF case-control cohort with 6.5-year follow up Scientific Reports, 12(1):18177.
PMID: 36307429 -
Species: Human
Sample Types: Plasma
C Lommatzsch et al. (2022-01-01), Elevated endothelin-1 levels as risk factor for an impaired ocular blood flow measured by OCT-A in glaucoma Scientific Reports, 12(1):11801.
PMID: 35821224 -
Species: Human
Sample Types: Plasma
A Orea-Tejad et al. (2022-01-01), Plasma Endothelial and Oxidative Stress Biomarkers Associated with Late Mortality in Hospitalized COVID-19 Patients Journal of Clinical Medicine, 11(14).
PMID: 35887714 -
Species: Mouse
Sample Types: Serum
N de Alwis et al. (2022-01-01), Actions of Esomeprazole on the Maternal Vasculature in Lean and Obese Pregnant Mice with Impaired Nitric Oxide Synthesis: A Model of Preeclampsia International Journal of Molecular Sciences, 23(15).
PMID: 35897759 -
Species: Human
Sample Types: Cell Culture Supernates
A Weigert et al. (2022-01-01), Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system Nature Communications, 13(1):6078.
PMID: 36241617 -
Species: Human
Sample Types: Plasma
JC Monroe et al. (2021-01-01), Acute effects of leg heat therapy on walking performance and cardiovascular and inflammatory responses to exercise in patients with peripheral artery disease Physiological Reports, 8(24):e14650.
PMID: 33369253 -
Species: Human
Sample Types: Serum
CVB Figueiredo et al. (2021-01-01), Priapism in sickle cell disease: Associations between NOS3 and EDN1 genetic polymorphisms and laboratory biomarkers PLoS ONE, 16(2):e0246067.
PMID: 33539452 -
Species: Human
Sample Types: Aqueous Humor
HM Kang et al. (2021-01-01), Significant elevation of aqueous endothelin-1 in central retinal vein occlusion PLoS ONE, 16(6):e0252530.
PMID: 34077461 -
Species: Human
Sample Types: Serum
T Kato et al. (2021-01-01), Preoperative Serum GDF-15, Endothelin-1 Levels, and Intraoperative Factors as Short-Term Operative Risks for Patients Undergoing Cardiovascular Surgery Journal of Clinical Medicine, 10(9).
PMID: 34063283 -
Species: Human
Sample Types: Plasma
Y Osawa et al. (2021-01-01), Blood angiopoietin-2 predicts liver angiogenesis and fibrosis in hepatitis C patients BMC gastroenterology, 21(1):55.
PMID: 33557759 -
Species: Mouse
Sample Types: Tissue Homogenates
GA Payne et al. (2021-01-01), Prolyl Endopeptidase contributes to early neutrophilic inflammation in acute cardiac transplant rejection JCI Insight, 0(0).
PMID: 33571164 -
Species: Human
Sample Types: Cell Culture Supernates
E Nassar et al. (2021-01-01), Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes Cancers, 13(10).
PMID: 34066023 -
Species: Human
Sample Types: Cell Culture Supernates
S Ríos-Arrab et al. (2021-01-01), Endothelin-1 as a Mediator of Heme Oxygenase-1-Induced Stemness in Colorectal Cancer: Influence of p53 Journal of personalized medicine, 11(6).
PMID: 34199777 -
Species: Rat
Sample Types: Plasma
Q Yu et al. (2021-01-01), Sorafenib not only impairs endothelium-dependent relaxation but also promotes vasoconstriction through the upregulation of vasoconstrictive endothelin type B receptors Toxicology and Applied Pharmacology, 414(0):115420.
PMID: 33503445 -
Species: Human
Sample Types: Plasma
I Dakota et al. (2021-01-01), Diagnostic prediction model in subjects with low-risk unstable angina pectoris/non-ST segment Elevation Myocardial Infarction European review for medical and pharmacological sciences, 25(16):5145-5152.
PMID: 34486689 -
Species: Human
Sample Types: Cell Culture Supernates
X Nie et al. (2021-01-01), Andrographolide Attenuates Established Pulmonary Hypertension via Rescue of Vascular Remodeling Biomolecules, 11(12).
PMID: 34944445 -
Species: Human
Sample Types: Cell Culture Supernates
M Velásquez et al. (2021-01-01), Differences in Endothelial Activation and Dysfunction Induced by Antiphospholipid Antibodies Among Groups of Patients With Thrombotic, Refractory, and Non-refractory Antiphospholipid Syndrome Frontiers in Physiology, 12(0):764702.
PMID: 34925061 -
Species: Human
Sample Types: Plasma
DL Wilson et al. (2020-01-01), Sleep-disordered breathing does not impact maternal outcomes in women with hypertensive disorders of pregnancy PLoS ONE, 15(4):e0232287.
PMID: 32339208 -
Species: Human
Sample Types: Serum
RP Santiago et al. (2020-01-01), TGFBR3 Polymorphisms (rs1805110 and rs7526590) Are Associated with Laboratory Biomarkers and Clinical Manifestations in Sickle Cell Anemia Dis Markers, 2020(0):8867986.
PMID: 33062074 -
Species: Human
Sample Types: Serum
J Chen et al. (2020-01-01), Hepatic lipocalin 2 promotes liver fibrosis and portal hypertension Scientific Reports, 10(1):15558.
PMID: 32968110 -
Species: Human
Sample Types: Plasma
A Schiappaca et al. (2020-01-01), Acute Effects of Metformin and Vildagliptin after a Lipid-Rich Meal on Postprandial Microvascular Reactivity in Patients with Type 2 Diabetes and Obesity: A Randomized Trial J Clin Med, 9(10).
PMID: 33050169 -
Species: Rat
Sample Types: Serum
MR Kim et al. (2020-01-01), Combination of Red Clover and Hops Extract Improved Menopause Symptoms in an Ovariectomized Rat Model Evid Based Complement Alternat Med, 2020(0):7941391.
PMID: 32595737 -
Species: Human
Sample Types: Cell Culture Supernates
JM Jung et al. (2020-01-01), Guanine Deaminase in Human Epidermal Keratinocytes Contributes to Skin Pigmentation Molecules, 25(11).
PMID: 32517074 -
Species: Human
Sample Types: Serum
Z Piotrowicz et al. (2020-01-01), Exercise-Induced Elevated BDNF Level Does Not Prevent Cognitive Impairment Due to Acute Exposure to Moderate Hypoxia in Well-Trained Athletes Int J Mol Sci, 21(15).
PMID: 32759658 -
Species: Human
Sample Types: Plasma
ZH Maayah et al. (2020-01-01), Breast cancer diagnosis is associated with relative left ventricular hypertrophy and elevated endothelin-1 signaling BMC Cancer, 20(1):751.
PMID: 32787791 -
Species: Human
Sample Types: Serum
M Castellazz et al. (2019-01-01), Increased Levels of Endothelin-1 in Cerebrospinal Fluid Are a Marker of Poor Visual Recovery after Optic Neuritis in Multiple Sclerosis Patients Dis. Markers, 2019(0):9320791.
PMID: 31583031 -
Species: Human
Sample Types: Urine
V Selvaraju et al. (2019-01-01), Urinary Biomarkers of Inflammation and Oxidative Stress Are Elevated in Obese Children and Correlate with a Marker of Endothelial Dysfunction Oxid Med Cell Longev, 2019(0):9604740.
PMID: 31737180 -
Species: Human
Sample Types: Cell Culture Supernates
SJ Mancini et al. (2018-01-01), Canagliflozin inhibits interleukin-1?-stimulated cytokine and chemokine secretion in vascular endothelial cells by AMP-activated protein kinase-dependent and -independent mechanisms Sci Rep, 8(1):5276.
PMID: 29588466 -
Species: Human
Sample Types: Plasma
AL D'Souza et al. (2018-01-01), Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence PLoS ONE, 13(3):e0194268.
PMID: 29547636 -
Species: Human
Sample Types: Serum
YH Li et al. (2018-01-01), Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease ? a cross-sectional study Sci Rep, 8(1):7378.
PMID: 29743680 -
Species: Rat
Sample Types: Serum
X Guo et al. (2018-01-01), Chronic ETAantagonist reverses hypertension and impairment of structure and function of peripheral small arteries in aortic stiffening Sci Rep, 8(1):3076.
PMID: 29449619 -
Species: Human
Sample Types: Cell Culture Supernates
I Roato et al. (2018-01-01), Adipose-Derived Stromal Vascular Fraction/Xenohybrid Bone Scaffold: An Alternative Source for Bone Regeneration Stem Cells Int, 2018(0):4126379.
PMID: 29853912 -
Species: Human
Sample Types: Serum
CD Ruediger et al. (2018-01-01), Influence of ethnic background on left atrial markers of inflammation, endothelial function and tissue remodelling Indian Pacing Electrophysiol J, 18(1):1-5.
PMID: 29477215 -
Species: Human
Sample Types: Plasma
LV Giles et al. (2018-01-01), The effect of low and high-intensity cycling in diesel exhaust on flow-mediated dilation, circulating NOx, endothelin-1 and blood pressure PLoS ONE, 13(2):e0192419.
PMID: 29466393 -
Species: Human
Sample Types: Serum
N Chebib et al. (2018-01-01), Dysregulation of the endothelin pathway in lymphangioleiomyomatosis with no direct effect on cell proliferation and migration Sci Rep, 8(1):14698.
PMID: 30279475 -
Species: Mouse
Sample Types: Tissue Homogenates
M Houde et al. (2018-01-01), Mouse Mast Cell Protease 4 Deletion Protects Heart Function and Survival After Permanent Myocardial Infarction Front Pharmacol, 9(0):868.
PMID: 30233357 -
Species: Human
Sample Types: Plasma
CK Wu et al. (2018-01-01), Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients J Clin Med, 7(11).
PMID: 30413105 -
Species: Human
Sample Types: Cell Culture Supernates
A Niewiarows et al. (2018-01-01), Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells PLoS ONE, 13(11):e0206443.
PMID: 30427893 -
Species: Rat
Sample Types: Serum
Y Kong et al. (2018-01-01), Trans-repression of NF?B pathway mediated by PPAR? improves vascular endothelium insulin resistance J. Cell. Mol. Med., 0(0).
PMID: 30398029 -
Species: Rat
Sample Types: Plasma
HIG Allam et al. (2018-01-01), The Potential Therapeutic Role of Peroxisome ProliferatorActivated Receptors Agonist in Preeclamptic Pregnant Rats J Coll Physicians Surg Pak, 28(1):31-35.
PMID: 29290188 -
Species: Human
Sample Types: Serum
DL McKay et al. (2018-01-01), A Pecan-Rich Diet Improves Cardiometabolic Risk Factors in Overweight and Obese Adults: A Randomized Controlled Trial Nutrients, 10(3).
PMID: 29534487 -
Species: Human
Sample Types: Cell Culture Supernates
M Caldeira-D et al. (2018-01-01), Preeclamptic plasma stimulates the expression of miRNAs, leading to a decrease in endothelin-1 production in endothelial cells Pregnancy Hypertens, 12(0):75-81.
PMID: 29674204 -
Species: Rat
Sample Types: Tissue Homogenates
F Mayyas et al. (2018-01-01), Cardiac effects of fish oil in a rat model of streptozotocin-induced diabetes Nutr Metab Cardiovasc Dis, 0(0).
PMID: 29615288 -
Species: Human
Sample Types: Plasma
J Lin et al. (2017-01-01), Peripheral venous congestion causes time- and dose-dependent release of endothelin-1 in humans Physiol Rep, 5(6).
PMID: 28320895 -
Species: Human
Sample Types: Plasma
A Treweeke et al. (2017-01-01), Preliminary study of hypoxia-related cardiovascular mediator-markers in patients with end-stage renal disease with and without diabetes and the effects of haemodialysis PLoS ONE, 12(5):e0178171.
PMID: 28542479 -
Species: Human
Sample Types: Cell Culture Supernates
C Wang et al. (2017-01-01), HMGB1 contributes to glomerular endothelial cell injury in ANCA-associated vasculitis through enhancing endothelium-neutrophil interactions J. Cell. Mol. Med, 0(0).
PMID: 28181422 -
Species: Human
Sample Types: Cell Culture Supernates
B Koehl et al. (2017-01-01), The endothelin B receptor plays a crucial role for the adhesion of neutrophils to the endothelium in sickle cell disease Haematologica, 0(0).
PMID: 28385784 -
Species: Human
Sample Types: Serum
W Khalid et al. (2017-01-01), Comparison of Serum Levels of Endothelin-1 in Chronic Periodontitis Patients Before and After Treatment J Clin Diagn Res, 11(4):ZC78-ZC81.
PMID: 28571268 -
Species: Human
Sample Types: Cell Culture Supernates
A Russignan et al. (2017-01-01), Endothelin-1 receptor blockade as new possible therapeutic approach in multiple myeloma Br. J. Haematol., 0(0).
PMID: 28597546 -
Species: Human
Sample Types: Plasma
Stephen N Davis (2016-01-01), Effects of equivalent sympathetic activation during hypoglycemia on endothelial function and pro-atherothrombotic balance in healthy individuals and obese standard treated type 2 diabetes Metab. Clin. Exp., 65(12):1695-1705.
PMID: 27832858 -
Species: Canine
Sample Types: Cell Culture Supernates
DL Michaels et al. (2016-01-01), Cellular microbiology of Mycoplasma canis Infect Immun, 0(0).
PMID: 27045036 -
Species: Human
Sample Types: Plasma
Koichi Nakazato (2016-01-01), Habitual cocoa intake reduces arterial stiffness in postmenopausal women regardless of intake frequency: a randomized parallel-group study Clin Interv Aging, 11(0):1645-1652.
PMID: 27881914 -
Species: Human
Sample Types: Serum
(2016-01-01), EDN1 Gene Variant is Associated with Neonatal Persistent Pulmonary Hypertension Sci Rep, 6(0):29877.
PMID: 27425626 -
Species: Human
Sample Types: Plasma
Shaowei Wu (2016-01-01), Chemical constituents and sources of ambient particulate air pollution and biomarkers of endothelial function in a panel of healthy adults in Beijing, China Sci. Total Environ, 560(0):141-9.
PMID: 27101449 -
Species: Mouse
Sample Types: Serum
Hou H et al. (2015-01-01), Citreoviridin Enhances Atherogenesis in Hypercholesterolemic ApoE-Deficient Mice via Upregulating Inflammation and Endothelial Dysfunction. PLoS ONE, 10(5):e0125956.
PMID: 25933220 -
Species: Human
Sample Types: Plasma
Pallis F et al. (2014-01-01), Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients. Br J Haematol, 164(2):286-95.
PMID: 24383847 -
Species: Human
Sample Types: Cell Culture Supernates
Liu B et al. (2014-01-01), Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells. PLoS ONE, 9(4):e93576.
PMID: 24727660 -
Species: Rat
Sample Types: Serum
Wang J et al. (2014-01-01), Tumor necrosis factor-alpha-induced reduction of glomerular filtration rate in rats with fulminant hepatic failure. Lab Invest, 94(7):740-51.
PMID: 24887412 -
Species: Human
Sample Types: Cell Culture Supernates
Wang J et al. (2013-01-01), The mechanism through which octreotide inhibits hepatic stellate cell activity. Mol Med Rep, 7(5):1559-64.
PMID: 23525276 -
Species: Mouse
Sample Types: Tissue Homogenates
Liu et al. (2013-01-01), TRPA1 controls inflammation and pruritogen responses in allergic contact dermatitis. FASEB J, 27(9):3549-63.
PMID: 23722916 -
Species: Human
Sample Types: Plasma
Sikkeland LI et al. (2012-01-01), Increased levels of inflammatory cytokines and endothelin-1 in alveolar macrophages from patients with chronic heart failure. PLoS ONE, 7(5):e36815.
PMID: 22615818 -
Species: Mouse
Sample Types: Serum
Sang et al. (2012-01-01), Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in ApoE-deficient mice. Lipids Health Dis, 11(0):166.
PMID: 23216643 -
Species: Human
Sample Types: Cell Culture Supernates
Jain A et al. (2012-01-01), Endothelin-1 induces endoplasmic reticulum stress by activating the PLC-IP(3) pathway: implications for placental pathophysiology in preeclampsia. Am. J. Pathol., 180(6):2309-20.
PMID: 22503784 -
Species: Human
Sample Types: Plasma
Rafnsson A et al. (2011-01-01), The endothelin receptor antagonist bosentan improves peripheral endothelial function in patients with type 2 diabetes mellitus and microalbuminuria: a randomised trial. Diabetologia, 55(3):600-7.
PMID: 22200728
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Customer Reviews for Endothelin-1 Quantikine ELISA Kit (7)
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7 customer ratings
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Sample Tested: Plasma,UrineVerified Customer | Posted 11/16/2022Plasma was in range at 4-fold dilution. Urine was in range at 2-fold dilution.
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Sample Tested: EDTA PlasmaVerified Customer | Posted 08/22/2019
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Sample Tested: UrineVerified Customer | Posted 12/17/2018
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Sample Tested: Serum and PlasmaVerified Customer | Posted 10/11/2018
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Sample Tested: UrineVerified Customer | Posted 09/11/2018
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Sample Tested: PlasmaVerified Customer | Posted 10/23/2017Plasma was used from male mice.
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Sample Tested: Serum and PlasmaVerified Customer | Posted 08/02/2017
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Product Documents for Endothelin-1 Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
