Human IL-8/CXCL8 QuantiGlo ELISA Kit

Name: Human IL-8/CXCL8 QuantiGlo ELISA Kit
Brand: R&D Systems
SKU: Q8000B
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # Q8000B

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(1)

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Key Product Details

Assay Length:	5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Sensitivity:	0.97 pg/mL
Assay Range:	1.6 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Human IL-8/CXCL8 QuantiGlo ELISA Kit Summary:	The QuantiGlo Human IL-8 Chemiluminescent Immunoassay is a 5 hour solid phase ELISA designed to measure human IL-8 levels in cell culture supernates, serum, and plasma. It contains the 72 amino acid variant of human IL-8 derived from E. coli as well as antibodies raised against this recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-8. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.

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Product Specifications for Human IL-8/CXCL8 QuantiGlo ELISA Kit

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IL-8
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision				Inter-Assay Precision
Sample	1	2	3	4	1	2	3	4
n	20	20	20	20	20	20	20	20
Mean (pg/mL)	3.66	22.5	228	2263	4.19	24.9	243	2393
Standard Deviation	0.241	1.02	8.98	146	0.481	1.57	14.7	216
CV%	6.6	4.5	3.9	6.5	11.5	6.3	6	9

Recovery

The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	98-107
Citrate Plasma (n=4)	101	97-107
EDTA Plasma (n=4)	105	95-113
Heparin Plasma (n=4)	107	102-113
Serum (n=4)	103	93-107

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-8 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IL-8/CXCL8 QuantiGlo ELISA Kit

	Human CXCL8/IL-8 Chemiluminescent ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent.

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells.

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1.

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

Item	Value
Long Name	Interleukin 8
Entrez Gene IDs	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names	3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP-1TSG-1, GCP1, IL-8, IL8, K60, LAI, LECT, LUCT, LYNAP, MDNCF, MDNCFb-ENAP, MONAP, MONAPGCP1, NAF, NAP-1NAP1, NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1, interleukin 8, member 8

Citations for Human IL-8/CXCL8 QuantiGlo ELISA Kit (14)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-8/CXCL8 QuantiGlo ELISA Kit include:

Species: Human
Sample Types: cell culture supernatant

X Ma et al. (2022-01-01), Lidocaine attenuates hypoxia/reoxygenation?induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway Molecular Medicine Reports, 25(5).
PMID: 35244190
Species: Human
Sample Types: Serum

KT Tang et al. (2022-01-01), Direct-Acting Antiviral Drugs Reduce Fibromyalgia Symptoms in Patients with Chronic Hepatitis C Journal of Clinical Medicine, 11(18).
PMID: 36142974
Species: Human
Sample Types: Serum

S Karampoor et al. (2021-01-01), The role of lovastatin in the attenuation of COVID-19 International immunopharmacology, 101(0):108192.
PMID: 34607230
Species: Human
Sample Types: Cell Culture Supernates

O Vaší?ek et al. (2020-01-01), Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model Harmful Algae, 96(0):101849.
PMID: 32560836
Species: Human
Sample Types: Cell Culture Supernates

M Sun et al. (2018-01-01), MicroRNA-874 inhibits TNF-?-induced remodeling in human fetal airway smooth muscle cells by targeting STAT3 Respir Physiol Neurobiol, 0(0).
PMID: 29448029
Species: Human
Sample Types: Cell Culture Supernates

A Abrahamsso et al. (2017-01-01), Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women Oncotarget, 8(54):92134-92142.
PMID: 29190903
Species: Human
Sample Types: Cell Culture Supernates

Rovillain E et al. (2011-01-01), Activation of nuclear factor-kappa B signalling promotes cellular senescence. Oncogene, 30(20):2356-66.
PMID: 21242976
Species: Human
Sample Types: Serum

Hatzistilianou M et al. (2010-01-01), Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia. Inflamm. Res., 59(5):339-47.
PMID: 19806318
Species: Human
Sample Types: Plasma

Zinner RG et al. (2007-01-01), Phase I clinical and pharmacodynamic evaluation of oral CI-1033 in patients with refractory cancer. Clin. Cancer Res., 13(10):3006-14.
PMID: 17505003
Species: Human
Sample Types: Whole Cells

Gessner C et al. (2007-01-01), Presence of cytokeratins in exhaled breath condensate of mechanical ventilated patients. Respir Med, 102(2):299-306.
PMID: 17996434
Species: Human
Sample Types: Cell Culture Supernates

Fichorova RN et al. (2006-01-01), Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. Infect. Immun., 74(10):5773-9.
PMID: 16988255
Species: Human
Sample Types: Plasma

Leitner JM et al. (2006-01-01), Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia. Clin. Pharmacol. Ther., 79(1):23-34.
PMID: 16413239
Species: Human
Sample Types: Plasma

Niessner A et al. (2005-01-01), Endurance training reduces circulating inflammatory markers in persons at risk of coronary events: impact on plaque stabilization? Atherosclerosis, 186(1):160-5.
PMID: 16084517
Species: Human
Sample Types: Plasma

Watt G et al. (2001-01-01), Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study. QJM, 94(11):599-607.
PMID: 11704689

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Human IL-8/CXCL8 QuantiGlo ELISA Kit

Name: Lu Bai

Sample Tested: Cell culture supernatant

Verified Customer | Posted 12/22/2021

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Human IL-8/CXCL8 QuantiGlo ELISA Kit

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Human IL-8/CXCL8 QuantiGlo ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Human IL-8/CXCL8 QuantiGlo ELISA Kit

Preparation and Storage

Background: IL-8/CXCL8

Citations for Human IL-8/CXCL8 QuantiGlo ELISA Kit (14)

Customer Reviews for Human IL-8/CXCL8 QuantiGlo ELISA Kit (1)

Human IL-8/CXCL8 QuantiGlo ELISA Kit

Product Documents for Human IL-8/CXCL8 QuantiGlo ELISA Kit

Product Datasheets

COA

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