Human/Mouse/Rat Activin A Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DAC00B
Key Product Details
Assay Length
5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL)
Sensitivity
7.85 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human/Mouse/Rat Activin A Quantikine ELISA Kit
The Quantikine Human/Mouse/Rat Activin A Immunoassay is a 5.0 hour solid-phase ELISA designed to measure Activin A in human, mouse, or rat cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human/mouse/rat Activin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/mouse/rat Activin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed Activin A.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Mouse, Rat
Specificity
Natural and recombinant human, mouse, and rat Activin A
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva - Samples were evaluated for the presence of human/mouse/rat
Activin A in this assay. No medical histories were available for the donors used in this study.
Cell Culture Supernates:
CMT-93 mouse rectal carcinoma cells were cultured in MEM with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until confluent. An aliquot was removed, assayed for human/mouse/rat Activin A, and measured 655 pg/mL.
| Human Samples | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=35) | 352 | 142-753 | 129 |
| EDTA plasma (n=35) | 319 | 115-665 | 124 |
| Heparin plasma (n=35) | 316 | 11-695 | 126 |
| Human Samples | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Saliva (n=13) | 192 | 85 | ND-428 |
| Mouse Samples | Mean (pg/mL) | Range (pg/mL | Standard Devation (pg/mL) |
| Serum (n=8) | 197 | 78.1-352 | 93.1 |
| EDTA plasma (n=9) | 254 | 136-471 | 84.2 |
| Heparin plasma (n=7) | 276 | 210-342 | 42.9 |
| Rat Samples | Mean (pg/mL) | Range pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=8) | 306 | 109-1466 | 469 |
| EDTA plasma (n=8) | 214 | 97.7-312 | 63.9 |
| Heparin plasma (n=7) | 276 | 210-342 | 42.9 |
Cell Culture Supernates:
Notw: Cell culture supernates were not corrected for the presence of fetal bovine serum.
IMR-90 human fetal lung fibroblasts were cultured in MEM with 10% fetal bovine serum until
confluent. An aliquot was removed, assayed for human/mouse/rat Activin A, and measured
5608 pg/mL.
WS-1 human fetal skin fibroblasts were cultured in MEM with 10% fetal bovine serum, NEAA,
and 2 mM L-glutamine until confluent. Cells were then stimulated for an additional 24 hours
with 100 ng/mL LPS, 10 ng/mL recombinant human IL-1 beta, or 10 ng/mL recombinant human
TNF-alpha. Aliquots were removed and assayed for levels of human/mouse/rat Activin A.
| Stimulant | Day 1 (pg/mL) |
| LPS | 8489 |
| IL-1 beta | 21,033 |
| TNF-alpha | 21,098 |
CMT-93 mouse rectal carcinoma cells were cultured in MEM with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until confluent. An aliquot was removed, assayed for human/mouse/rat Activin A, and measured 655 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 101 | 295 | 498 | 106 | 308 | 529 |
| Standard Deviation | 4.2 | 12.5 | 21.9 | 8.4 | 15.5 | 24.8 |
| CV% | 4.2 | 4.2 | 4.4 | 7.9 | 5.0 | 4.7 |
Recovery for Human/Mouse/Rat Activin A Quantikine ELISA Kit
The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 93-114 |
| EDTA Plasma (n=4) | 110 | 95-115 |
| Heparin Plasma (n=4) | 107 | 99-113 |
| Serum (n=4) | 109 | 100-115 |
Linearity
To assess the linearity of the assay, human samples containing and/or spiked with high concentrations of Activin A were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human/Mouse/Rat Activin A Quantikine ELISA Kit
Human/Mouse/Rat Activin A ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Activin A
Activin A is an important member of the TGF-beta superfamily that is produced by many different cell types and is essential for proper development. Bioactive Activin A exists as a disulfide-linked homodimer that recognizes and binds to four Activin receptors, Activin RIA and RIB, and Activin RIIA and RIIB. This ligand-receptor binding event triggers activation of SMAD signaling pathways ultimately regulating a wide variety of functions including stem cell maintenance, self-renewal, and differentiation, B-cell differentiation and proliferation, wound healing, metabolism, and gonadal development.
Activin A is first produced as precursor protein containing a propeptide that gets proteolytically cleaved off leaving the mature A chain. Two A chains assemble by disulfide bond formation creating the bioactive homodimer. The bioavailability of Activin A is regulated by cell-associated decoy molecules (BAMBI, Betaglycan, Cripto), secreted proteins (a2-Macroglobulin, Follistatin, FLRG), and intracellular incorporation of the beta A subunit into Activin AC or Inhibin A. Experimentally, recombinant Activin A is commonly used to maintain proliferative potential of human induced pluripotent stem cells and to promote differentiation of embryonic stem cells into endoderm and pancreatic B cells.
Alternate Names
activin AB alpha polypeptide, Activin beta-A chain, erythroid differentiation factor, Erythroid differentiation protein, follicle-stimulating hormone-releasing protein, FSH-releasing protein, inhibin beta A chain, inhibin beta A subunit, Inhibin, beta-1
Gene Symbol
INHBA
Additional Activin A Products
Product Documents for Human/Mouse/Rat Activin A Quantikine ELISA Kit
Product Specific Notices for Human/Mouse/Rat Activin A Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.