Human CXCL16 Quantikine ELISA Kit

Name: Human CXCL16 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DCX160
Price: 615 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DCX160

Citations (14)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.017 ng/mL
Assay Range:	0.156 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human CXCL16 Quantikine ELISA Kit Summary:	The Quantikine Human CXCL16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CXCL16 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CXCL16 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CXCL16 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CXCL16.

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Product Specifications for Human CXCL16 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human CXCL16
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	42	42
Mean (pg/mL)	0.92	2.85	6.33	1	3.3	7.3
Standard Deviation	0.04	0.1	0.31	0.1	0.3	0.7
CV%	4.3	3.5	4.9	10	9.1	9.6

Recovery

The recovery of CXCL16 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	93-110
EDTA Plasma (n=4)	102	94-112
Heparin Plasma (n=4)	97	85-105
Serum (n=4)	103	89-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CXCL16 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human CXCL16 Quantikine ELISA Kit

	Human CXCL16 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.

Item	Value
Entrez Gene IDs	58191 (Human); 66102 (Mouse)
Alternate Names	CXC chemokine ligand 16, CXCL16, CXCLG16, SCYB16, SR-PSOXTransmembrane chemokine CXCL16, SRPSOXC-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, chemokine (C-X-C motif) ligand 16

Citations for Human CXCL16 Quantikine ELISA Kit (14)

Showing 1 - 10 of 14 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CXCL16 Quantikine ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

C Helissey et al. (2022), Two New Potential Therapeutic Approaches in Radiation Cystitis Derived from Mesenchymal Stem Cells: Extracellular Vesicles and Conditioned Medium Biology, 11(7).
PMID: 36101361
Species: Human
Sample Types: Plasma

T Guan et al. (2022), Platelet SR-PSOX/CXCL16-CXCR6 Axis Influences Thrombotic Propensity and Prognosis in Coronary Artery Disease International Journal of Molecular Sciences, 23(19).
PMID: 36232370
Species: Human
Sample Types: Cell Culture Supernates

Z Ma et al. (2021), Role of CXCL16 in BLM-induced epithelial-mesenchymal transition in human A549 cells Respiratory Research, 22(1):42.
PMID: 33549109
Species: Human
Sample Types: Plasma

K Prajzlerov et al. (2021), High miR-451 expression in peripheral blood mononuclear cells from subjects at risk of developing rheumatoid arthritis Scientific Reports, 11(1):4719.
PMID: 33633196
Species: Human
Sample Types: Serum

FG Scurt et al. (2019), Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 4(10):1373-1386.
PMID: 31701047
Species: Human
Sample Types: Cell Culture Supernates

RJ Chen et al. (2017), Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 8(37):62081-62098.
PMID: 28977928
Species: Human
Sample Types: Cell Culture Supernates

Gosuke Takiguchi et al. (2016), Wnt5a-Ror2 signaling in mesenchymal stem cells promotes proliferation of gastric cancer cells by activating CXCL16-CXCR6 axis. Cancer Science, 0(0):1349-7006.
PMID: 26708384
Species: Human
Sample Types: Cell Culture Supernates

Zeng M et al. (2012), Suppression of NF-kappaB Reduces Myocardial No-Reflow. PLoS ONE, 7(10):e47306.
PMID: 23056624
Species: Human
Sample Types: Cell Culture Supernates

Santhekadur PK et al. (2012), Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor ÃŽÂºB and miR-221. J. Biol. Chem., 287(17):13952-8.
PMID: 22396537
Species: Human
Sample Types: Plasma

Huang M et al. (2010), An intron polymorphism in the CXCL16 gene is associated with increased risk of coronary artery disease in Chinese Han population: a large angiography-based study. Atherosclerosis, 210(1):160-5.
PMID: 19954776
Species: Human
Sample Types: Serum

Wente MN et al. (2008), Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma. Int. J. Oncol., 33(2):297-308.
PMID: 18636150
Species: Human
Sample Types: Serum

Sung SY et al. (2008), Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis. Cancer Res., 68(23):9996-10003.
PMID: 19047182
Species: Human
Sample Types: Cell Culture Supernates

Held-Feindt J et al. (2008), Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas. Glia, 56(7):764-74.
PMID: 18293410
Species: Human
Sample Types: Cell Culture Supernates

Smith C et al. (2008), High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins. Cardiovasc. Res., 79(1):195-203.
PMID: 18339644

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.