Human FGF acidic/FGF1 DuoSet ELISA

Name: Human FGF acidic/FGF1 DuoSet ELISA
Brand: R&D Systems
SKU: DY232
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY232

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human FGF acidic/FGF1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human FGF acidic/FGF1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF acidic. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human FGF acidic/FGF1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human FGF acidic/FGF1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human FGF acidic/FGF1 DuoSet ELISA

	Human FGF acidic ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Item	Value
Long Name	Fibroblast Growth Factor acidic
Entrez Gene IDs	2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
Alternate Names	AFGF, ECGF, ECGF-betaAcidic fibroblast growth factor, ECGFB, FGF acidic, FGF-1, FGF-alpha, FGFABeta-endothelial cell growth factor, GLIO703, HBGF-1, HBGF1, alpha, alpha-ECGF, beta-ECGF, endothelial cell growth factor, beta, fibroblast growth factor 1 (acidic), heparin-binding growth factor 1

Citations for Human FGF acidic/FGF1 DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGF acidic/FGF1 DuoSet ELISA include:

Species: Mouse
Sample Types: Serum

T Keeley et al. (2019), Resistance to visceral obesity is associated with increased locomotion in mice expressing an endothelial cell-specific fibroblast growth factor 1 transgene Physiol Rep, 7(7):e14034.
PMID: 30972920

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