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Human CCL3/MIP-1 alpha Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Length: | 3.5 hours or 4.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (10 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL) |
| Sample Types: | Cell Culture Supernates |
| Sensitivity: | 10 pg/mL |
| Assay Range: |
31.2 - 1,000 pg/mL (Cell Culture Supernates) 46.9 - 1,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma) |
| Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary: | The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha. |
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Human CCL3/MIP-1 alpha ELISA Cell Culture Supernate Standard Curve |
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Human CCL3/MIP-1 alpha ELISA Serum/Plasma Standard Curve |
Citations for Human CCL3/MIP-1 alpha Quantikine ELISA Kit (60)
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Citations are publications that use Bio-Techne products. Selected citations for Human CCL3/MIP-1 alpha Quantikine ELISA Kit include:
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Species: Human
Sample Types: Vitreous Humor
T Tsai et al. (2023-01-01), Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy PLoS ONE, 18(1):e0280488.
PMID: 36662891 -
Species: Human
Sample Types: Serum
E Terpos et al. (2022-01-01), Daratumumab Improves Bone Turnover in Relapsed/Refractory Multiple Myeloma; Phase 2 Study "REBUILD" Oncogene, 14(11).
PMID: 35681747 -
Species: Human
Sample Types: Cell Culture Supernates
Z Liu et al. (2021-01-01), Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma BMC Cancer, 21(1):732.
PMID: 34174847 -
Species: Human
Sample Types: Gingival Crevicular Fluid
ML Mannakanda et al. (2021-01-01), Effect of ultrasonic scaling with adjunctive photodynamic therapy in the treatment of gingival inflammation among diabetic patients undergoing fixed orthodontic treatment Photodiagnosis and photodynamic therapy, 0(0):102360.
PMID: 34052421 -
Species: Human
Sample Types: Serum
L Avila et al. (2021-01-01), Cognitive functional therapy (CFT) compared with core training exercise (CTE) in patients with failed back surgery syndrome (FBSS): A study protocol for a randomized controlled trial Journal of bodywork and movement therapies, 26(0):428-434.
PMID: 33992278 -
Species: Human
Sample Types: Serum
E Terpos et al. (2021-01-01), Carfilzomib Improves Bone Metabolism in Patients with Advanced Relapsed/Refractory Multiple Myeloma: Results of the CarMMa Study Cancers, 13(6).
PMID: 33809268 -
Species: Human
Sample Types: Plasma
S Sendic et al. (2021-01-01), B cell and monocyte phenotyping: A quick asset to investigate the immune status in patients with IgA nephropathy PLoS ONE, 16(3):e0248056.
PMID: 33740017 -
Species: Human
Sample Types: Sputum
E Pandolfi et al. (2021-01-01), Cytokine expression patterns in hospitalized children with Bordetella pertussis, Rhinovirus or co-infection Scientific Reports, 11(1):10948.
PMID: 34040002 -
Species: Human
Sample Types: Cell Culture Supernates
L Sadeghi et al. (2020-01-01), Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells Cancers (Basel), 12(5).
PMID: 32370190 -
Species: Human
Sample Types: Cell Culture Supernates
M Dampmann et al. (2020-01-01), CpG stimulation of chronic lymphocytic leukemia cells induces a polarized cell shape and promotes migration in vitro and in vivo PLoS ONE, 15(2):e0228674.
PMID: 32040489 -
Species: Human
Sample Types: Serum
T Peeters et al. (2020-01-01), An observational study of innate immune responses in patients with acute appendicitis Sci Rep, 10(1):17352.
PMID: 33060696 -
Species: Human
Sample Types: Cell Culture Supernates
T Kodama et al. (2020-01-01), CCL3-CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways Lab. Invest., 0(0).
PMID: 32457351 -
Species: Human
Sample Types: Plasma
M Sivina et al. (2020-01-01), CXCL13 plasma levels function as a biomarker for disease activity in patients with chronic lymphocytic leukemia Leukemia, 0(0).
PMID: 33087831 -
Species: Human
Sample Types: Serum
J Gerosa-Net et al. (2020-01-01), High- and moderate-intensity training modify LPS-induced ex-vivo interleukin-10 production in obese men in response to an acute exercise bout Cytokine, 136(0):155249.
PMID: 32829109 -
Species: Human
Sample Types: Cell Culture Supernates
W Wang et al. (2019-01-01), Platelet-secreted CCL3 and its receptor CCR5 promote invasive and migratory abilities of anaplastic thyroid carcinoma cells via MMP-1 Cell. Signal., 0(0):109363.
PMID: 31344439 -
Species: Human
Sample Types: Serum
N Aziz et al. (2019-01-01), Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 20(1):33.
PMID: 31521107 -
Species: Human
Sample Types: Serum
YL Zhang et al. (2018-01-01), A cross-sectional study: serum CCL3/MIP-1? levels may reflect lumbar intervertebral disk degeneration in Han Chinese people J Pain Res, 11(0):497-503.
PMID: 29551909 -
Species: Human
Sample Types: Cell Culture Supernates
A Stiff et al. (2018-01-01), Nitric Oxide Production by Myeloid Derived Suppressor Cells Plays a Role in Impairing Fc Receptor-Mediated Natural Killer Cell Function Clin. Cancer Res., 0(0).
PMID: 29363526 -
Species: Human
Sample Types: Cell Culture Supernates
N Zhang et al. (2017-01-01), A novel orally available Syk/Src/Jak2 inhibitor, SKLB-850, showed potent anti-tumor activities in B cell lymphoma (BCL) models Oncotarget, 8(67):111495-111507.
PMID: 29340070 -
Species: Human
Sample Types: Cell Culture Supernates
(2016-01-01), Functional Differences between IgM and IgD Signaling in Chronic Lymphocytic Leukemia J Immunol, 0(0).
PMID: 27534555 -
Species: Human
Sample Types: Cell Culture Supernates
Balakrishnan K et al. (2015-01-01), The phosphoinositide-3-kinase (PI3K)-delta and gamma inhibitor, IPI-145 (Duvelisib), overcomes signals from the PI3K/AKT/S6 pathway and promotes apoptosis in CLL. Leukemia, 29(9):1811-22.
PMID: 25917267 -
Species: Human
Sample Types: Cell Culture Supernates
Hashimoto A et al. (2014-01-01), Low concentrations of recombinant granulocyte macrophage-colony stimulating factor derived from Chinese hamster ovary cells augments long-term bioactivity with delayed clearance in vitro. Cytokine, 68(2):118-26.
PMID: 24813650 -
Species: Human
Sample Types: Cell Culture Supernates
Johnson TR et al. (2012-01-01), Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 86(3):1339-47.
PMID: 22090124 -
Species: Human
Sample Types: BALF
Quesnel C et al. (2012-01-01), Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury. Crit. Care Med., 40(1):21-8.
PMID: 21926612 -
Species: Human
Sample Types: Plasma
Ponader S et al. (2012-01-01), The Bruton tyrosine kinase inhibitor PCI-32765 thwarts chronic lymphocytic leukemia cell survival and tissue homing in vitro and in vivo. Blood, 119(5):1182-9.
PMID: 22180443 -
Species: Human
Sample Types: Plasma
Sivina M et al. (2011-01-01), CCL3 (MIP-1alpha) plasma levels and the risk for disease progression in chronic lymphocytic leukemia. Blood, 117(5):1662-9.
PMID: 21115978 -
Species: Human
Sample Types: Serum
Su Y et al. (2010-01-01), A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets. Clin. Cancer Res., 16(1):348-57.
PMID: 20028756 -
Species: Human
Sample Types: Cell Culture Supernates
Mabilleau G et al. (2009-01-01), Interleukin-32 promotes osteoclast differentiation but not osteoclast activation. PLoS ONE, 4(1):e4173.
PMID: 19137064 -
Species: Human
Sample Types: Plasma
Calmy A et al. (2009-01-01), HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial. AIDS, 23(8):929-39.
PMID: 19425222 -
Species: Human
Sample Types: Cell Culture Supernates
Aldinucci D et al. (2008-01-01), Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int. J. Cancer, 122(4):769-76.
PMID: 17935139 -
Species: Human
Sample Types: Cell Culture Supernates
Le Quement C et al. (2008-01-01), MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 294(6):L1076-84.
PMID: 18390828 -
Species: Human
Sample Types: Cell Culture Supernates
Pene J et al. (2008-01-01), Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes. J. Immunol., 180(0):7423.
PMID: 18490742 -
Species: Human
Sample Types: Serum
Pokkali S et al. (2008-01-01), Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions. Cytokine, 41(3):307-14.
PMID: 18226915 -
Species: Human
Sample Types: Plasma
Mayr FB et al. (2008-01-01), Duffy antigen modifies the chemokine response in human endotoxemia. Crit. Care Med., 36(1):159-65.
PMID: 18007272 -
Species: Human
Sample Types: Cell Culture Supernates
Olsnes AM et al. (2008-01-01), In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22. J. Interferon Cytokine Res., 28(5):297-310.
PMID: 18547160 -
Species: Human
Sample Types: Cell Culture Supernates
Martinez de la Torre Y et al. (2007-01-01), Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6. Proc. Natl. Acad. Sci. U.S.A., 104(7):2319-24.
PMID: 17283337 -
Species: Human
Sample Types: BALF
Kang MJ et al. (2007-01-01), IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation. J. Immunol., 178(3):1948-59.
PMID: 17237446 -
Species: Human
Sample Types: Cell Lysates
Tikhonov I et al. (2006-01-01), Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES. Int. Immunol., 18(8):1243-51.
PMID: 16740603 -
Species: Human
Sample Types: Cell Culture Supernates
Abonyo BO et al. (2006-01-01), Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells. Cytokine, 36(5):237-44.
PMID: 17350279 -
Species: Human
Sample Types: BALF
Capelli A et al. (2005-01-01), CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis. Eur. Respir. J., 25(4):701-7.
PMID: 15802346 -
Species: Human
Sample Types: Cell Culture Supernates
Wong CK et al. (2005-01-01), Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB. Am. J. Respir. Cell Mol. Biol., 33(2):186-94.
PMID: 15860795 -
Species: Human
Sample Types: Cell Culture Supernates
Choi ES et al. (2004-01-01), Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia. Am. J. Respir. Crit. Care Med., 170(5):508-15.
PMID: 15191918 -
Species: Human
Sample Types: CSF
Grygorczuk S et al. (2004-01-01), Concentrations of macrophage inflammatory proteins MIP-1alpha and MIP-1beta and interleukin 8 (il-8) in lyme borreliosis. Infection, 32(6):350-5.
PMID: 15597225 -
Species: Human
Sample Types: Cell Culture Supernates
Emori Y et al. (2004-01-01), Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages. J. Gen. Virol., 85(0):2603-13.
PMID: 15302954 -
Species: Human
Sample Types: Sputum
Parameswaran K et al. (2004-01-01), Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma. J. Allergy Clin. Immunol., 114(1):73-9.
PMID: 15241347 -
Species: Human
Sample Types: Serum
Wang CR et al. (2004-01-01), Up-regulation of XCR1 expression in rheumatoid joints. Rheumatology (Oxford), 43(5):569-73.
PMID: 14970401 -
Species: Human
Sample Types: Cell Culture Supernates
Wang CR et al. (2003-01-01), Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis. Clin. Exp. Immunol., 132(2):371-8.
PMID: 12699431 -
Species: Human
Sample Types: Plasma
Milliken D et al. (2002-01-01), Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites. Clin. Cancer Res., 8(4):1108-14.
PMID: 11948121 -
Species: Human
Sample Types: Cell Culture Supernates
Ciesielski CJ et al. (2002-01-01), TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion. Eur. J. Immunol., 32(7):2037-45.
PMID: 12115625 -
Species: Human
Sample Types: Cell Culture Supernates
Messmer D et al. (2002-01-01), Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells. J. Immunol., 169(8):4172-82.
PMID: 12370346 -
Species: Human
Sample Types: Cell Culture Supernates
Binninger-Schinzel D et al. (2002-01-01), Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. , 294(1):209-21.
PMID: 11886279 -
Species: Human
Sample Types: Cell Culture Supernates
Honczarenko M et al. (2002-01-01), CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Blood, 100(7):2321-9.
PMID: 12239139 -
Species: Human
Sample Types: Cell Culture Supernates
Loeuillet C et al. (2001-01-01), Distinct hematopoietic support by two human stromal cell lines. Exp. Hematol., 29(6):736-45.
PMID: 11378269 -
Species: Human
Sample Types: Cell Culture Supernates
Hurst SM et al. (2001-01-01), Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity, 14(6):705-14.
PMID: 11420041 -
Species: Human
Sample Types: Cell Culture Supernates
Yates SL et al. (2000-01-01), Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia. J. Neurochem., 74(3):1017-25.
PMID: 10693932 -
Species: Human
Sample Types: Serum
Bottcher MF et al. (2000-01-01), Chemoattractant factors in breast milk from allergic and nonallergic mothers. Pediatr. Res., 47(5):592-7.
PMID: 10813582 -
Species: Primate
Sample Types: Cell Culture Supernates
Gauduin MC et al. (1999-01-01), Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production. Proc. Natl. Acad. Sci. U.S.A., 96(24):14031-6.
PMID: 10570193 -
Species: Primate - Pan troglodytes (Chimpanzee)
Sample Types: Plasma
Lauw FN et al. (1999-01-01), Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees. J. Infect. Dis., 179(3):646-52.
PMID: 9952371 -
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
Lehner T et al. (1996-01-01), Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques. , 2(7):767-75.
PMID: 8673922 -
Species: Human
Sample Types: Cell Culture Supernates
Wang J et al. (), VSIG-3 as a ligand of VISTA inhibits human T-cell function. Immunolog156(1):74-85.
PMID: 30220083
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Customer Reviews for Human CCL3/MIP-1 alpha Quantikine ELISA Kit (2)
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Sample Tested: BMECVerified Customer | Posted 07/11/2019
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/14/2016
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Product Documents for Human CCL3/MIP-1 alpha Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
