Human PDGF-AB Quantikine ELISA Kit

Name: Human PDGF-AB Quantikine ELISA Kit
Brand: R&D Systems
SKU: DHD00C

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DHD00C

Citations (40)

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Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Sample Types:	Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
Sensitivity:	3.83 pg/mL
Assay Range:	15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Human PDGF-AB Quantikine ELISA Kit Summary:	The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.

View Full Technical Details for Human PDGF-AB Quantikine ELISA Kit

Product Specifications for Human PDGF-AB Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human PDGF-AB
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	92.7	312	656	102	343	701
Standard Deviation	8.28	9.73	15.2	10.9	29.9	52.3
CV%	8.9	3.1	2.3	10.7	8.7	7.5

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	107	350	726	119	362	723
Standard Deviation	6.95	10.9	63.1	10.7	20.9	54.5
CV%	6.5	3.1	8.7	9	5.8	7.5

Recovery

The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	105	93-119
Platelet-poor EDTA Plasma (n=4)	104	78-117
Platelet-poor Heparin Plasma (n=4)	105	86-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human PDGF-AB Quantikine ELISA Kit

	Human PDGF-AB ELISA Cell Culture Supernate Standard Curve
	Human PDGF-AB ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-AB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Item	Value
Long Name	Platelet-derived Growth Factor AB
Alternate Names	PDGF-AB, PDGFAB

Citations for Human PDGF-AB Quantikine ELISA Kit (40)

Showing 1 - 10 of 40 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-AB Quantikine ELISA Kit include:

Species: Human
Sample Types: Plasma

N Casagrande et al. (2021), In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel International Journal of Molecular Sciences, 22(6).
PMID: 33809553
Species: Human
Sample Types: Plasma

L Kobari et al. (2021), Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells Leukemia, 0(0).
PMID: 34556797
Species: Human
Sample Types: Whole Cells

B Walters et al. (2021), Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells Cells, 10(11).
PMID: 34831345
Species: Human
Sample Types: Serum

A Michalak et al. (2021), Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients BioMed Research International, 2021(0):8867985.
PMID: 33644233
Species: Human
Sample Types: Serum

A Michalak et al. (2020), Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales World Journal of Gastroenterology, 26(47):7538-7549.
PMID: 33384553
Species: Human
Sample Types: Cell Culture Supernates

F Agostini et al. (2018), 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures PLoS ONE, 13(9):e0203048.
PMID: 30188924
Species: Human
Sample Types: Plasma

S Pauly et al. (2018), The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff BMC Musculoskelet Disord, 19(1):422.
PMID: 30497435
S Rossi et al. (2018), A novel dressing for the combined delivery of platelet lysate and vancomycin hydrochloride to chronic skin ulcers: Hyaluronic acid particles in alginate matrices Eur J Pharm Sci, 118(0):87-95.
PMID: 29574078
Species: Human
Sample Types: Cell Culture Supernates

K Nagaishi et al. (2017), Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy Sci Rep, 7(1):8484.
PMID: 28814814
Species: Human
Sample Types: Plasma

B Shen et al. (2017), Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion Med. Sci. Monit., 23(0):3578-3584.
PMID: 28734116
Species: Human
Sample Types: Plasma

E Tóth-Zsámb et al. (2017), Cardiac rehabilitation programme as a non-pharmacological platelet inhibitory tool in acute coronary syndrome survivors Eur J Prev Cardiol, 0(0):2047487317704.
PMID: 28438028
Species: Human
Sample Types: Cell Lysates

M Bernardi et al. (2017), The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro J Transl Med, 15(1):90.
PMID: 28460641
Species: Human
Sample Types: Cell Culture Supernates

(2016), Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling Sci Rep, 6(0):29029.
PMID: 27374240
Species: Human
Sample Types: Cell Culture Supernates

Anne Fischer-Ni (2016), Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates Cytotherapy, 0(0).
PMID: 27887865
Species: Human
Sample Types: Plasma

Gorka Orive (2016), Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts Eur. J. Obstet. Gynecol. Reprod. Biol., 206(0):125-130.
PMID: 27693932
Species: Human
Sample Types: Cell Culture Supernates

Chou M et al. (2014), Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PLoS ONE, 9(6):e99145.
PMID: 24945500
Species: Human
Sample Types: Cell Culture Supernates

Iudicone et al. (2014), Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells. J Transl Med, 12(0):28.
PMID: 24467837
Species: Human
Sample Types: Plasma

Kageyama Y et al. (2014), Involvement of Rac in thromboxane A2induced human platelet activation: regulation of sCD40 ligand release and PDGFAB secretion. Mol Med Rep, 10(1):107-12.
PMID: 24737161
Species: Human
Sample Types: Cell Lysates

Barsotti M et al. (2013), Effect of platelet lysate on human cells involved in different phases of wound healing. PLoS ONE, 8(12):e84753.
PMID: 24386412
Species: Human
Sample Types: Cell Lysates

Kinzebach S et al. (2013), Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol, 14(0):48.
PMID: 24168020
Species: Human
Sample Types: Cell Lysates

Rauch C et al. (2011), Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX, 28(4):305-16.
PMID: 22130485
Species: Human
Sample Types: Platelet Poor Plasma

Ding W et al. (2010), Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood, 116(16):2984-93.
PMID: 20606160
Species: Human
Sample Types: Plasma

Mathew P et al. (2008), Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer. Br. J. Cancer, 99(9):1426-32.
PMID: 18841158
Species: Human
Sample Types: Platelet Poor Plasma

Kanter J et al. (2008), Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates. Clin. Cancer Res., 14(12):3942-7.
PMID: 18559616
Species: Porcine
Sample Types: Cell Culture Supernates

Siegbahn A et al. (2007), TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler. Thromb. Vasc. Biol., 28(1):135-41.
PMID: 17991872
Species: Human
Sample Types: Cell Culture Supernates

Yashiro M et al. (2007), Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int. J. Cancer, 120(3):686-93.
PMID: 17096355
Species: Human
Sample Types: Cell Culture Supernates

Tejada ML et al. (2006), Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin. Cancer Res., 12(9):2676-88.
PMID: 16675559
Species: Human
Sample Types: Cell Culture Supernates

Pegorier S et al. (2006), Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells. J. Immunol., 177(7):4861-9.
PMID: 16982928
Species: Human
Sample Types: Cell Culture Supernates

Nagineni CN et al. (2005), Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 203(1):35-43.
PMID: 15368539
Species: Rabbit
Sample Types: Plasma

Zhang CQ et al. (2004), Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit. Chin. Med. J., 117(12):1853-5.
PMID: 15603719
Species: Human
Sample Types: Complex Sample Type

Thorn JJ et al. (2004), Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery. Int J Oral Maxillofac Surg, 33(1):95-100.
PMID: 14690664
Species: Human
Sample Types: Cell Culture Supernates

Saika S et al. (2004), Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab. Invest., 84(10):1245-58.
PMID: 15273699
Species: Porcine
Sample Types: Cell Lysates

Bracamonte MP et al. (2002), Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. Am. J. Physiol. Heart Circ. Physiol., 283(3):H853-60.
PMID: 12181111
Species: Porcine
Sample Types: Cell Culture Supernates

Seeger FH et al. (2002), Fibrinogen induces chemotactic activity in endothelial cells. , 176(2):109-15.
PMID: 12354170
Species: Human
Sample Types: Cell Culture Supernates

Skutek M et al. (2001), Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts. Eur. J. Appl. Physiol., 86(1):48-52.
PMID: 11820322
Species: Human
Sample Types: Cell Culture Supernates

Westphal JR et al. (2000), Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo. Int. J. Cancer, 86(6):768-76.
PMID: 10842189
Species: Human
Sample Types: Plasma

Landesberg R et al. (2000), Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J. Oral Maxillofac. Surg., 58(3):297.
PMID: 10716112
Species: Canine
Sample Types: Serum

Vieweg U et al. (1999), Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhage in dogs. Acta Neurochir (Wien), 141(8):861-5.
PMID: 10536723
Species: Human
Sample Types: Serum

Soubrier M et al. (1999), Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome. Am. J. Kidney Dis., 34(4):633-8.
PMID: 10516342
Species: Canine
Sample Types: Cell Culture Supernates

Gu JW et al. (1997), Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells. Am. J. Physiol., 273(2):H628-33.
PMID: 9277477

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.