Human PDGF-AB Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 4.5 hours |
Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL) |
Sample Types: | Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma |
Sensitivity: | 3.83 pg/mL |
Assay Range: | 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma) |
Human PDGF-AB Quantikine ELISA Kit Summary: | The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB. |
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Citations for Human PDGF-AB Quantikine ELISA Kit (40)
Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-AB Quantikine ELISA Kit include:
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Species: Human
Sample Types: Plasma
N Casagrande et al. (2021), In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel International Journal of Molecular Sciences, 22(6).
PMID: 33809553 -
Species: Human
Sample Types: Plasma
L Kobari et al. (2021), Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells Leukemia, 0(0).
PMID: 34556797 -
Species: Human
Sample Types: Whole Cells
B Walters et al. (2021), Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells Cells, 10(11).
PMID: 34831345 -
Species: Human
Sample Types: Serum
A Michalak et al. (2021), Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients BioMed Research International, 2021(0):8867985.
PMID: 33644233 -
Species: Human
Sample Types: Serum
A Michalak et al. (2020), Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales World Journal of Gastroenterology, 26(47):7538-7549.
PMID: 33384553 -
Species: Human
Sample Types: Cell Culture Supernates
F Agostini et al. (2018), 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures PLoS ONE, 13(9):e0203048.
PMID: 30188924 -
Species: Human
Sample Types: Plasma
S Pauly et al. (2018), The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff BMC Musculoskelet Disord, 19(1):422.
PMID: 30497435 -
S Rossi et al. (2018), A novel dressing for the combined delivery of platelet lysate and vancomycin hydrochloride to chronic skin ulcers: Hyaluronic acid particles in alginate matrices Eur J Pharm Sci, 118(0):87-95.
PMID: 29574078 -
Species: Human
Sample Types: Cell Culture Supernates
K Nagaishi et al. (2017), Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy Sci Rep, 7(1):8484.
PMID: 28814814 -
Species: Human
Sample Types: Plasma
B Shen et al. (2017), Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion Med. Sci. Monit., 23(0):3578-3584.
PMID: 28734116 -
Species: Human
Sample Types: Plasma
E Tóth-Zsámb et al. (2017), Cardiac rehabilitation programme as a non-pharmacological platelet inhibitory tool in acute coronary syndrome survivors Eur J Prev Cardiol, 0(0):2047487317704.
PMID: 28438028 -
Species: Human
Sample Types: Cell Lysates
M Bernardi et al. (2017), The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro J Transl Med, 15(1):90.
PMID: 28460641 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling Sci Rep, 6(0):29029.
PMID: 27374240 -
Species: Human
Sample Types: Cell Culture Supernates
Anne Fischer-Ni (2016), Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates Cytotherapy, 0(0).
PMID: 27887865 -
Species: Human
Sample Types: Plasma
Gorka Orive (2016), Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts Eur. J. Obstet. Gynecol. Reprod. Biol., 206(0):125-130.
PMID: 27693932 -
Species: Human
Sample Types: Cell Culture Supernates
Chou M et al. (2014), Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PLoS ONE, 9(6):e99145.
PMID: 24945500 -
Species: Human
Sample Types: Cell Culture Supernates
Iudicone et al. (2014), Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells. J Transl Med, 12(0):28.
PMID: 24467837 -
Species: Human
Sample Types: Plasma
Kageyama Y et al. (2014), Involvement of Rac in thromboxane A2induced human platelet activation: regulation of sCD40 ligand release and PDGFAB secretion. Mol Med Rep, 10(1):107-12.
PMID: 24737161 -
Species: Human
Sample Types: Cell Lysates
Barsotti M et al. (2013), Effect of platelet lysate on human cells involved in different phases of wound healing. PLoS ONE, 8(12):e84753.
PMID: 24386412 -
Species: Human
Sample Types: Cell Lysates
Kinzebach S et al. (2013), Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol, 14(0):48.
PMID: 24168020 -
Species: Human
Sample Types: Cell Lysates
Rauch C et al. (2011), Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX, 28(4):305-16.
PMID: 22130485 -
Species: Human
Sample Types: Platelet Poor Plasma
Ding W et al. (2010), Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood, 116(16):2984-93.
PMID: 20606160 -
Species: Human
Sample Types: Plasma
Mathew P et al. (2008), Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer. Br. J. Cancer, 99(9):1426-32.
PMID: 18841158 -
Species: Human
Sample Types: Platelet Poor Plasma
Kanter J et al. (2008), Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates. Clin. Cancer Res., 14(12):3942-7.
PMID: 18559616 -
Species: Porcine
Sample Types: Cell Culture Supernates
Siegbahn A et al. (2007), TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler. Thromb. Vasc. Biol., 28(1):135-41.
PMID: 17991872 -
Species: Human
Sample Types: Cell Culture Supernates
Yashiro M et al. (2007), Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int. J. Cancer, 120(3):686-93.
PMID: 17096355 -
Species: Human
Sample Types: Cell Culture Supernates
Tejada ML et al. (2006), Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin. Cancer Res., 12(9):2676-88.
PMID: 16675559 -
Species: Human
Sample Types: Cell Culture Supernates
Pegorier S et al. (2006), Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells. J. Immunol., 177(7):4861-9.
PMID: 16982928 -
Species: Human
Sample Types: Cell Culture Supernates
Nagineni CN et al. (2005), Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 203(1):35-43.
PMID: 15368539 -
Species: Rabbit
Sample Types: Plasma
Zhang CQ et al. (2004), Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit. Chin. Med. J., 117(12):1853-5.
PMID: 15603719 -
Species: Human
Sample Types: Complex Sample Type
Thorn JJ et al. (2004), Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery. Int J Oral Maxillofac Surg, 33(1):95-100.
PMID: 14690664 -
Species: Human
Sample Types: Cell Culture Supernates
Saika S et al. (2004), Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab. Invest., 84(10):1245-58.
PMID: 15273699 -
Species: Porcine
Sample Types: Cell Lysates
Bracamonte MP et al. (2002), Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. Am. J. Physiol. Heart Circ. Physiol., 283(3):H853-60.
PMID: 12181111 -
Species: Porcine
Sample Types: Cell Culture Supernates
Seeger FH et al. (2002), Fibrinogen induces chemotactic activity in endothelial cells. , 176(2):109-15.
PMID: 12354170 -
Species: Human
Sample Types: Cell Culture Supernates
Skutek M et al. (2001), Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts. Eur. J. Appl. Physiol., 86(1):48-52.
PMID: 11820322 -
Species: Human
Sample Types: Cell Culture Supernates
Westphal JR et al. (2000), Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo. Int. J. Cancer, 86(6):768-76.
PMID: 10842189 -
Species: Human
Sample Types: Plasma
Landesberg R et al. (2000), Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J. Oral Maxillofac. Surg., 58(3):297.
PMID: 10716112 -
Species: Canine
Sample Types: Serum
Vieweg U et al. (1999), Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhage in dogs. Acta Neurochir (Wien), 141(8):861-5.
PMID: 10536723 -
Species: Human
Sample Types: Serum
Soubrier M et al. (1999), Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome. Am. J. Kidney Dis., 34(4):633-8.
PMID: 10516342 -
Species: Canine
Sample Types: Cell Culture Supernates
Gu JW et al. (1997), Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells. Am. J. Physiol., 273(2):H628-33.
PMID: 9277477
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Product Documents for Human PDGF-AB Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.