Human EGF Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 3.5 hours or 4.5 hours |
Sample Type & Volume Required Per Well: | Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL) |
Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine |
Sensitivity: | 0.7 pg/mL |
Assay Range: | 3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine) |
Human EGF Quantikine ELISA Kit Summary: | The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF. |
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Citations for Human EGF Quantikine ELISA Kit (72)
Citations are publications that use Bio-Techne products. Selected citations for Human EGF Quantikine ELISA Kit include:
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Species: Human
Sample Types: Urine
P Ngamjanyap et al. (2022), Predicting treatment response and clinicopathological findings in lupus nephritis with urine epidermal growth factor, monocyte chemoattractant protein-1 or their ratios PLoS ONE, 17(3):e0263778.
PMID: 35271583 -
Species: Human
Sample Types: Cell Lysates
E Razani et al. (2022), A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture Journal of virological methods, 0(0):114478.
PMID: 35101406 -
Species: Human
Sample Types: Serum
V Sabrina et al. (2022), Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing International Journal of Molecular Sciences, 23(18).
PMID: 36142617 -
Species: Human
Sample Types: Urine
P Janssens et al. (2021), Enhanced MCP-1 Release in Early Autosomal Dominant Polycystic Kidney Disease Kidney international reports, 6(6):1687-1698.
PMID: 34169210 -
Species: Human
Sample Types: Plasma
N Casagrande et al. (2021), In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel International Journal of Molecular Sciences, 22(6).
PMID: 33809553 -
Species: Human
Sample Types: Cell Culture Supernates
JKC Kre beta et al. (2021), NRF2 Enables EGFR Signaling in Melanoma Cells International Journal of Molecular Sciences, 22(8).
PMID: 33916908 -
Species: Human
Sample Types: Plasma
S Isozaki et al. (2021), Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators Mediators of Inflammation, 2021(0):5582943.
PMID: 33859537 -
Species: Human
Sample Types: Plasma
HP Li et al. (2021), Combination of Epithelial Growth Factor Receptor Blockers and CDK4/6 Inhibitor for Nasopharyngeal Carcinoma Treatment Cancers, 13(12).
PMID: 34204797 -
Species: Human
Sample Types: Plasma
L Kobari et al. (2021), Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells Leukemia, 0(0).
PMID: 34556797 -
Species: Human
Sample Types: Cell Culture Supernates
DH Shin et al. (2021), Oncogenic KRAS promotes growth of lung cancer cells expressing SLC3A2-NRG1 fusion via ADAM17-mediated shedding of NRG1 Oncogene, 0(0).
PMID: 34743207 -
Species: Human
Sample Types: Cord Blood Serum
S Di Marco et al. (2020), Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas Biomolecules, 10(5).
PMID: 32354031 -
Species: Human
Sample Types: Tissue Homogenates
H Guo et al. (2020), Expression, Purification and Functional Identification of the Modified hEGF Protein Protein Expr Purif, 179(0):105787.
PMID: 33197614 -
Species: Human
Sample Types: Cell Culture Supernates
J Codony-Ser et al. (2020), Anti-epidermal growth factor vaccine antibodies increase the antitumor activity of kinase inhibitors in ALK and RET rearranged lung cancer cells Transl Oncol, 14(1):100887.
PMID: 33129112 -
Species: Human
Sample Types: Cell Culture Supernates
CM Garvey et al. (2020), Anti-EGFR Therapy Induces EGF Secretion by Cancer-Associated Fibroblasts to Confer Colorectal Cancer Chemoresistance Cancers (Basel), 12(6).
PMID: 32481658 -
Species: Human
Sample Types: Cell Culture Supernates
S Kim et al. (2020), LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells Autophagy, 0(0):1-20.
PMID: 32762616 -
Species: Human
Sample Types: Tissue Homogenates
O Pogozhykh et al. (2020), Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology Int J Mol Sci, 21(11).
PMID: 32512889 -
Species: Human
Sample Types: Cell Culture Supernates
ZJ Liang et al. (2019), Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro Cell Transplant, 0(0):9636897198256.
PMID: 30675799 -
Species: Human
Sample Types: Cell Culture Supernates
ASMR Haque et al. (2019), CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production Sci Rep, 9(1):14611.
PMID: 31601953 -
Species: Human
Sample Types: Whole Cells
Z Dong et al. (2019), MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells Cancer Med, 0(0).
PMID: 31691527 -
Species: Human
Sample Types: Serum
P Xing et al. (2018), Therapeutic cancer vaccine: phase I clinical tolerance study of Hu-rhEGF-rP64k/Mont in patients with newly diagnosed advanced non-small cell lung cancer BMC Immunol., 19(1):14.
PMID: 29661145 -
Species: Human
Sample Types: Cell Culture Supernates
C Saury et al. (2018), Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches Stem Cell Res Ther, 9(1):128.
PMID: 29720259 -
Species: Human
Sample Types: Urine
B Satirapoj et al. (2018), Urinary epidermal growth factor, monocyte chemoattractant protein-1 or their ratio as predictors for rapid loss of renal function in type 2 diabetic patients with diabetic kidney disease BMC Nephrol, 19(1):246.
PMID: 30241508 -
Species: Human
Sample Types: Cell Culture Supernates
F Agostini et al. (2018), 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures PLoS ONE, 13(9):e0203048.
PMID: 30188924 -
Species: Human
Sample Types: Saliva
N Azuma et al. (2018), Deterioration in saliva quality in patients with Sj�gren's syndrome: impact of decrease in salivary epidermal growth factor on the severity of intraoral manifestations Inflamm Regen, 38(0):6.
PMID: 29657585 -
Species: Human
Sample Types: Plasma
S Chandran et al. (2017), Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction J Am Heart Assoc, 6(5).
PMID: 28468787 -
Species: Human
Sample Types: Cell Lysates
M Bernardi et al. (2017), The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro J Transl Med, 15(1):90.
PMID: 28460641 -
Species: Human
Sample Types: Cell Culture Supernates
L Shen et al. (2017), C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers Exp Ther Med, 13(6):3021-3031.
PMID: 28587375 -
Species: Human
Sample Types: Cell Culture Supernates
K Nagaishi et al. (2017), Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy Sci Rep, 7(1):8484.
PMID: 28814814 -
Species: Human
Sample Types: Cell Culture Supernates
A Soeda et al. (2017), The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking Oncotarget, 8(20):33316-33328.
PMID: 28410196 -
Species: Human
Sample Types: Plasma
AL Garner et al. (2017), Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation PLoS ONE, 12(7):e0181214.
PMID: 28746392 -
Species: Human
Sample Types: Tissue Homogenates
YY Go et al. (2017), Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts PLoS ONE, 12(8):e0182716.
PMID: 28797129 -
Species: Human
Sample Types: Plasma
Jan Pierce et al. (2017), Comparative analyses of industrial-scale human platelet lysate preparations. Transfusion, 0(0):1537-2995.
PMID: 28990195 -
Species: Human
Sample Types: Urine
Supanat Worawichaw (2016), Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis Kidney Blood Press. Res, 41(6):997-1007.
PMID: 27988512 -
Species: Human
Sample Types: Serum
(2016), Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome Int J Mol Sci, 17(8).
PMID: 27527149 -
Species: Human
Sample Types: Plasma
Gorka Orive (2016), Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts Eur. J. Obstet. Gynecol. Reprod. Biol., 206(0):125-130.
PMID: 27693932 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia Stem Cell Res Ther, 7(1):128.
PMID: 27612459 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4 Sci Rep, 6(0):33507.
PMID: 27641064 -
Species: Human
Sample Types: Cell Culture Supernates
Anne Fischer-Ni (2016), Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates Cytotherapy, 0(0).
PMID: 27887865 -
Species: Plant - Arabidopsis
Sample Types: Protein
Yonghua He (2016), Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF) PLoS ONE, 11(6):e0157034.
PMID: 27314851 -
Species: Human
Sample Types: Cell Culture Supernates
Zhang Z et al. (2014), Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer Res, 74(10):2869-81.
PMID: 24686166 -
Species: Human
Sample Types: Cell Culture Supernates
Chou M et al. (2014), Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PLoS ONE, 9(6):e99145.
PMID: 24945500 -
Species: Human
Sample Types: Serum
Dickinson R et al. (2014), The evolution of cellular deficiency in GATA2 mutation. Blood, 123(6):863-74.
PMID: 24345756 -
Species: Human
Sample Types: Plasma
Llovet JM et al. (2012), Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma. Clin. Cancer Res., 18(8):2290-300.
PMID: 22374331 -
Species: Human
Sample Types: Cell Culture Supernates
Tanizaki J et al. (2012), Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer. Clin Cancer Res, 18(22):6219-26.
PMID: 22843788 -
Species: Human
Sample Types: Serum
(2012), Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. J. Thromb. Haemost., 10(8):1616-23.
PMID: 22537155 -
Species: Human
Sample Types: Serum
Aronis KN et al. (2011), Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans. Diabetologia, 54(9):2358-67.
PMID: 21660636 -
Species: Human
Sample Types: Serum
Rahbari NN et al. (2011), Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer. BMC Cancer, 11(0):286.
PMID: 21729304 -
Species: Human
Sample Types: Cell Lysates
Rauch C et al. (2011), Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX, 28(4):305-16.
PMID: 22130485 -
Species: Human
Sample Types: Urine
Cherney DZ et al. (2009), Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study. Diabetes Care, 32(1):91-3.
PMID: 18945921 -
Species: Human
Sample Types: Urine
Soler-Garcia AA et al. (2009), A urinary biomarker profile for children with HIV-associated renal diseases. Kidney Int., 76(2):207-14.
PMID: 19357719 -
Species: Human
Sample Types: Plasma
Debucquoy A et al. (2009), Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer. J. Clin. Oncol., 27(17):2751-7.
PMID: 19332731 -
Species: Human
Sample Types: Cell Culture Supernates
Sexton DJ et al. (2009), Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases. Biochem. J., 422(2):383-92.
PMID: 19527222 -
Species: Human
Sample Types: Nasal Lavage Fluid
Lee HS et al. (2009), Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis. Am. J. Respir. Crit. Care Med., 180(11):1056-67.
PMID: 19762561 -
Species: Human
Sample Types: Cell Culture Supernates
Farrow B et al. (2009), Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2. J. Surg. Res., 156(1):155-60.
PMID: 19592030 -
Species: Human
Sample Types: Serum
Han SW et al. (2009), Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer. Br. J. Cancer, 100(2):298-304.
PMID: 19127259 -
Species: Human
Sample Types: Cell Culture Supernates
Ren W et al. (2008), Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo. Clin. Cancer Res., 14(9):2785-2795.
PMID: 18451246 -
Species: Human
Sample Types: Serum
Garcia B et al. (2008), Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine. Clin. Cancer Res., 14(3):840-6.
PMID: 18245547 -
Species: Human
Sample Types: Milk
Kverka M et al. (2007), Cytokine profiling in human colostrum and milk by protein array. Clin. Chem., 53(5):955-62.
PMID: 17363422 -
Species: Human
Sample Types: Serum
Lemos-Gonzalez Y et al. (2007), Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma. Br. J. Cancer, 96(10):1569-78.
PMID: 17453000 -
Species: Human
Sample Types: Follicular Fluid
Asimakopoulos B et al. (2007), The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection. Fertil. Steril., 90(1):60-4.
PMID: 17980366 -
Species: Human
Sample Types: Cell Culture Supernates
Yashiro M et al. (2007), Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int. J. Cancer, 120(3):686-93.
PMID: 17096355 -
Species: Human
Sample Types: Cell Culture Supernates
Dolloff NG et al. (2007), Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor. Cancer Res., 67(2):555-62.
PMID: 17234763 -
Species: Human
Sample Types: Saliva
Balicki R et al. (2005), Salivary epidermal growth factor in oral cavity cancer. Oral Oncol., 41(1):48-55.
PMID: 15598585 -
Species: Human
Sample Types: Cell Culture Supernates
Groschl M et al. (2005), Salivary leptin induces increased expression of growth factors in oral keratinocytes. J. Mol. Endocrinol., 34(2):353-66.
PMID: 15821102 -
Species: Human
Sample Types: Cell Lysates
Nishishita T et al. (2004), A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells. Biochem. Biophys. Res. Commun., 325(1):24-31.
PMID: 15522196 -
Species: Human
Sample Types: Plasma
Jahanshahi G et al. (2004), Alterations in antioxidant power and levels of epidermal growth factor and nitric oxide in saliva of patients with inflammatory bowel diseases. Dig. Dis. Sci., 49(11):1752-7.
PMID: 15628697 -
Species: Human
Sample Types: Urine
Chiou YY et al. (2004), Factors associated with the outcomes of children with unilateral ureteropelvic junction obstruction. J. Urol., 171(1):397-402; disc.
PMID: 14665942 -
Species: Human
Sample Types: Hydrosalpinx Fluid
Chen CD et al. (2002), The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx. Hum. Reprod., 17(1):128-33.
PMID: 11756375 -
Species: Rat
Sample Types: Cell Culture Supernates
Truckenmiller ME et al. (2002), AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression. Exp. Neurol., 175(0):318.
PMID: 12061863 -
Species: Human
Sample Types: Saliva
Nagler RM et al. (2002), Saliva analysis in the clinical setting: revisiting an underused diagnostic tool. 1333-1343.e7, 50(3):214-25.
PMID: 12033287 -
Species: Human
Sample Types: Nasal Lavage Fluid
Benson M et al. (2002), Epithelial cells in nasal fluids from patients with allergic rhinitis: how do they relate to epidermal growth factor, eosinophils and eosinophil cationic protein? Acta Otolaryngol., 122(2):202-5.
PMID: 11936914 -
Species: Human
Sample Types: Serum
Shin CE et al. (2000), Diminished epidermal growth factor levels in infants with necrotizing enterocolitis. J. Pediatr. Surg., 35(2):173-6; discus.
PMID: 10693661
There are no citations that match your criteria.
Customer Reviews for Human EGF Quantikine ELISA Kit (5)
Customer Images
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Sample Tested: Plasma,Serum,UrineVerified Customer | Posted 03/15/2022We analyzed urine at a 150-fold dilution and plasma/serum at a 10-fold dilution. The standard curve had an excellent 4PL fit. We continue to use this assay routinely.
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Sample Tested: SerumVerified Customer | Posted 12/11/2021
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Sample Tested: 293T human embryonic kidney cell line,T47D human breast cancer cell lineVerified Customer | Posted 09/13/2018
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Sample Tested: SerumVerified Customer | Posted 06/12/2018
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Sample Tested: Adult thymocytesVerified Customer | Posted 04/03/2018
There are no reviews that match your criteria.
Product Documents for Human EGF Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.