Human IGFBP-2 Quantikine ELISA Kit

Name: Human IGFBP-2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DGB200
Price: 615 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DGB200

Citations (5)

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Key Product Details

Assay Length:	3.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Sensitivity:	0.09 ng/mL
Assay Range:	0.313 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Human IGFBP-2 Quantikine ELISA Kit Summary:	The Quantikine Human IGFBP-2 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGFBP-2 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human IGFBP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-2.

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Product Specifications for Human IGFBP-2 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IGFBP-2
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1.96	6.24	12.7	2.24	6.93	13.6
Standard Deviation	0.07	0.31	0.63	0.17	0.31	0.62
CV%	3.6	5	5	7.6	4.5	4.6

Recovery

The recovery of IGFBP-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=8)	100	88-113
Urine (n=4)	109	94-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of IGFBP-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IGFBP-2 Quantikine ELISA Kit

	Human IGFBP-2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-2

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Item	Value
Long Name	Insulin-like Growth Factor Binding Protein 2
Entrez Gene IDs	3485 (Human); 16008 (Mouse)
Alternate Names	BP2, IBP-2, IBP2, IGF-BP53, IGF-binding protein 2, IGFBP-2, IGFBP2, insulin-like growth factor binding protein 2 (36kD), insulin-like growth factor binding protein 2, 36kDa, insulin-like growth factor-binding protein 2

Citations for Human IGFBP-2 Quantikine ELISA Kit (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IGFBP-2 Quantikine ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

M Shimizu et al. (2021-01-01), Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression Cancers, 13(18).
PMID: 34572779
Species: Human
Sample Types: Serum

P Fahlbusch et al. (2020-01-01), Physiological Disturbance in Fatty Liver Energy Metabolism Converges on IGFBP2 Abundance and Regulation in Mice and Men Int J Mol Sci, 21(11).
PMID: 32532003
Species: Human
Sample Types: Cell Culture Supernates

S Sakimoto et al. (2017-01-01), CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect JCI Insight, 2(2):e89906.
PMID: 28138561
Species: Human
Sample Types: Plasma

Qibin Zhang (2016-01-01), Temporal profiles of plasma proteome during childhood development J Proteomics, 152(0):321-328.
PMID: 27890796
Species: Human
Sample Types: Cell Culture Supernates

Kiss K et al. (2015-01-01), Chronic hyperglycemia induces trans-differentiation of human pancreatic stellate cells and enhances the malignant molecular communication with human pancreatic cancer cells. PLoS ONE, 10(5):e0128059.
PMID: 26010611

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Showing 1 - 5 of 5 citations Showing All

Product Documents for Human IGFBP-2 Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.