Human KGF/FGF-7 DuoSet ELISA

Name: Human KGF/FGF-7 DuoSet ELISA
Brand: R&D Systems
SKU: DY251
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY251

Citations (7)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	31.2 - 2,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human KGF/FGF-7 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human KGF/FGF-7 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human KGF/FGF-7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human KGF/FGF-7 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human KGF/FGF-7 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human KGF/FGF-7 DuoSet ELISA

	Human KGF / FGF-7 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.

Item	Value
Long Name	Keratinocyte Growth Factor
Entrez Gene IDs	2252 (Human); 403915 (Canine)
Alternate Names	FGF-7, FGF7, HBGF-7, HBGF7, Heparin-binding growth factor 7, KGF, KGFfibroblast growth factor 7 (keratinocyte growth factor), fibroblast growth factor 7HBGF-7, keratinocyte growth factor

Citations for Human KGF/FGF-7 DuoSet ELISA (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human KGF/FGF-7 DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

P Ebner-Peki et al. (2021), Self-assembly of differentiated progenitor cells facilitates spheroid human skin organoid formation and planar skin regeneration Theranostics, 11(17):8430-8447.
PMID: 34373751
Species: Human
Sample Types: Cell Culture Supernates

A Suhail et al. (2019), DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells Cell Rep, 29(11):3522-3538.e7.
PMID: 31825833
Species: Human
Sample Types: Cell Culture Supernates

KA Correll et al. (2018), TGF beta inhibits HGF, FGF7, and FGF10 expression in normal and IPF lung fibroblasts Physiol Rep, 6(16):e13794.
PMID: 30155985
Species: Human
Sample Types: Cell Culture Supernates

J Ikari et al. (2017), Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease PLoS ONE, 12(9):e0184039.
PMID: 28880936
Species: Transgenic Mouse
Sample Types: Serum

Zheng C et al. (2011), Prevention of radiation-induced salivary hypofunction following hKGF gene delivery to murine submandibular glands. Clin. Cancer Res., 17(9):2842-51.
PMID: 21367751
Species: Human
Sample Types: BALF

Quesnel C et al. (2007), Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 294(2):L334-43.
PMID: 18065658
Species: Human
Sample Types: Ascites Fluid

Steele IA et al. (2001), Induction of FGF receptor 2-IIIb expression and response to its ligands in epithelial ovarian cancer. Oncogene, 20(41):5878-87.
PMID: 11593393

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