Human FGF basic/FGF2/bFGF Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DFB50
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit
Product Specifications
Measurement
Detection Method
Conjugate
Reactivity
Specificity
Cross-reactivity
Interference
Sample Values
Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
Serum | ND | 0 | ND |
EDTA plasma | 13.5 | 10 | ND-14.6 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 28.8 | 104 | 234 | 32.8 | 108 | 229 |
Standard Deviation | 2.8 | 3.2 | 7.1 | 3.0 | 8.0 | 17.3 |
CV% | 9.7 | 3.1 | 3.0 | 9.1 | 7.4 | 7.6 |
Recovery for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit
The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 108 | 94-119 |
EDTA Plasma (n=5) | 103 | 90-124 |
Serum (n=5) | 106 | 86-124 |
Linearity
To assess the linearity of the assay, the following samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and then assayed.

Scientific Data Images for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit
Human FGF basic ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: FGF basic/FGF2/bFGF
FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
Long Name
Alternate Names
Gene Symbol
Additional FGF basic/FGF2/bFGF Products
Product Documents for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit
Product Specific Notices for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.