Human Endostatin Quantikine ELISA Kit

Name: Human Endostatin Quantikine ELISA Kit
Brand: R&D Systems
SKU: DNST0

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DNST0

Citations (22)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Sensitivity:	0.063 ng/mL
Assay Range:	0.31 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Human Endostatin Quantikine ELISA Kit Summary:	The Quantikine Human Endostatin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Endostatin in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human Endostatin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Endostatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Endostatin.

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Product Specifications for Human Endostatin Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human Endostatin
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	0.7	2.02	4.14	0.76	2.13	4.21
Standard Deviation	0.05	0.12	0.15	0.06	0.13	0.24
CV%	6.9	6	3.6	7.9	6.1	5.7

Recovery

The recovery of Endostatin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	91-114
EDTA Plasma (n=4)	100	92-110
Heparin Plasma (n=4)	98	86-108
Serum (n=4)	100	93-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Endostatin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Endostatin Quantikine ELISA Kit

	Human Endostatin ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endostatin

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

Item	Value
Entrez Gene IDs	80781 (Human); 12822 (Mouse)
Alternate Names	COL18A1, Endostatin, FLJ27325, FLJ34914, KNO, KNO1MGC74745, KS, Knobloch syndrome, type 1, antiangiogenic agent, collagen alpha-1(XVIII) chain, collagen, type XVIII, alpha 1, human type XVIII collagen10endostatin, multi-functional protein MFP

Citations for Human Endostatin Quantikine ELISA Kit (22)

Showing 1 - 10 of 22 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Endostatin Quantikine ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

J Zhang et al. (2019), Selectively activated PRP exerts differential effects on tendon stem/progenitor cells and tendon healing J Tissue Eng, 10(0):2.04173E+15.
PMID: 30728936
Species: Human
Sample Types: Serum

FG Scurt et al. (2019), Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 4(10):1373-1386.
PMID: 31701047
Species: Human
Sample Types: Serum

YY Joo et al. (2019), Circulating pro- and anti-angiogenic factors in multi-stage liver disease and hepatocellular carcinoma progression Sci Rep, 9(1):9137.
PMID: 31235729
Species: Human
Sample Types: Plasma

M Holsti et al. (2018), Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue Eur J Vasc Endovasc Surg, 0(0).
PMID: 29656960
Species: Human
Sample Types: Serum

Borghini A et al. (2015), Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide. PLoS ONE, 10(6):e0130166.
PMID: 26076019
Species: Human
Sample Types: Serum

Marek-Trzonkowska N et al. (2015), Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors. PLoS ONE, 10(5):e0126190.
PMID: 25951297
Species: Human
Sample Types: Cell Culture Supernates

Hutzen et al. (2014), Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin. BMC Cancer, 14(0):206.
PMID: 24646176
Species: Human
Sample Types: Serum

Sponder M et al. (2014), Exercise increases serum endostatin levels in female and male patients with diabetes and controls. Cardiovasc Diabetol, 13(0):6.
PMID: 24393402
Species: Human
Sample Types: Whole Cells

Battinelli et al. (2014), Anticoagulation inhibits tumor cell-mediated release of platelet angiogenic proteins and diminishes platelet angiogenic response. Blood, 123(1):101-12.
PMID: 24065244
Species: Mouse
Sample Types: Tissue Homogenates

Leifler K et al. (2013), Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer. J Immunol, 190(8):4420-30.
PMID: 23509357
Species: Human
Sample Types: Cell Culture Supernates

Zheng et al. (2012), Antitumor activities of human placenta-derived mesenchymal stem cells expressing endostatin on ovarian cancer. PLoS ONE, 7(7):e39119.
PMID: 22911684
Species: Human
Sample Types: Serum

Landt S et al. (2011), Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer. Anticancer Res., 31(8):2651-5.
PMID: 21778318
Species: Human
Sample Types: Serum

Landt S et al. (2011), In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer. Anticancer Res., 31(8):2609-15.
PMID: 21778312
Species: Goat
Sample Types: Cell Culture Supernates

Sun XD et al. (2009), Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds. Biomaterials, 30(6):1222-31.
PMID: 19059640
Species: Human
Sample Types: Plasma

Ohlund D et al. (2008), Expression pattern and circulating levels of endostatin in patients with pancreas cancer. Int. J. Cancer, 122(12):2805-10.
PMID: 18360823
Species: Human
Sample Types: Plasma

Holmes CE et al. (2008), Tamoxifen and aromatase inhibitors differentially affect vascular endothelial growth factor and endostatin levels in women with breast cancer. Clin. Cancer Res., 14(10):3070-6.
PMID: 18483373
Species: Human
Sample Types: Tissue Homogenates

Bendrik C et al. (2008), Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo. Cancer Res., 68(9):3405-12.
PMID: 18451168
Species: Human
Sample Types: Serum

Mellon MJ et al. (2008), Suppression of renal cell carcinoma growth and metastasis with sustained antiangiogenic gene therapy. Hum. Gene Ther., 19(5):487-95.
PMID: 18507514
Species: Human
Sample Types: Serum

Li HL et al. (2007), Pharmacokinetic and pharmacodynamic study of intratumoral injection of an adenovirus encoding endostatin in patients with advanced tumors. Gene Ther., 15(4):247-56.
PMID: 18097470
Species: Human
Sample Types: Serum

Ferrandina G et al. (2006), Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer. Clin. Cancer Res., 12(7):2055-60.
PMID: 16609015
Species: Human
Sample Types: Serum

Furstenberger G et al. (2006), Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer. Br. J. Cancer, 94(4):524-31.
PMID: 16450002
Species: Human
Sample Types: Cell Culture Supernates

Perryman LA et al. (2006), Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo. Biochem. Biophys. Res. Commun., 345(3):1207-14.
PMID: 16723121

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.