Human Coagulation Factor III/Tissue Factor DuoSet ELISA

Name: Human Coagulation Factor III/Tissue Factor DuoSet ELISA
Brand: R&D Systems
SKU: DY2339
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY2339

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	7.81 - 500 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Coagulation Factor III/Tissue Factor DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Coagulation Factor III/Tissue Factor DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Coagulation Factor III/Tissue Factor. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Coagulation Factor III/Tissue Factor DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Coagulation Factor III/Tissue Factor DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Coagulation Factor III/Tissue Factor DuoSet ELISA

	Human Coagulation Factor III / Tissue Factor ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.

Item	Value
Entrez Gene IDs	2152 (Human); 14066 (Mouse)
Alternate Names	CD142, CD142 antigen, Coagulation Factor III, F3, FLJ17960, TF, TFA, Thromboplastin, Tissue Factor, coagulation factor III (thromboplastin, tissue factor)

Citations for Human Coagulation Factor III/Tissue Factor DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Coagulation Factor III/Tissue Factor DuoSet ELISA include:

Species: Human
Sample Types: Cell Lysates

TLR Humphries et al. (2021-01-01), PAR2-Induced Tissue Factor Synthesis by Primary Cultures of Human Kidney Tubular Epithelial Cells Is Modified by Glucose Availability International Journal of Molecular Sciences, 22(14).
PMID: 34299151
Species: Mouse
Sample Types: Cell Culture Supernates

F Cheng et al. (2020-01-01), Agomelatine Attenuates Isoflurane-Induced Inflammation and Damage in Brain Endothelial Cells Drug Design, Development and Therapy, 14(0):5589-5598.
PMID: 33376303
Species: Bovine
Sample Types: Whole Cells

Ji Eun Kim (2016-01-01), Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation PLoS ONE, 11(6):e0156763.
PMID: 27258428

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