Human Thrombospondin-2 DuoSet ELISA

Name: Human Thrombospondin-2 DuoSet ELISA
Brand: R&D Systems
SKU: DY1635
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY1635

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Thrombospondin-2 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Thrombospondin-2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Thrombospondin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Thrombospondin-2 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Thrombospondin-2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Thrombospondin-2 DuoSet ELISA

	Human Thrombospondin-2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Thrombospondin-2

Thrombospondins (TSP) are secreted multidomain glycoproteins with many putative functions including modulating cell adhesion, proliferation, migration, and angiogenesis. At least five TSPs exist: TSP-1, -2, -3, -4, and cartilage oligomeric glycoprotein (COMP)/TSP-5. The family can be divided into two subgroups. The first, TSP-1 and -2, are homotrimeric molecules, each monomer containing an N-terminal/heparin-binding domain, a coiled-coil oligomerization domain, a von Willebrand factor-type/procollagen homology domain, three type 1 thrombospondin repeats (TSR), three EGF-like repeats, seven TSP type 3 repeats, and a C-terminal globular domain. TSP-1 and -2 are thought to mediate interactions between cells and the extracellular matrix, and as such are matricellular proteins. In contrast, TSP-3, -4, and COMP/TSP-5 are homopentamers that lack the N-terminal structure of TSP-1 and -2, but have a similar arrangement of EGF and TSP type 3 repeats, and the C-terminal domain characteristic of the TSP family.

Item	Value
Entrez Gene IDs	7058 (Human)
Alternate Names	THBS2, TSP-2, TSP2thrombospondin-2, Thrombospondin-2, Thrombospondin2, thrombospondin 2

Citations for Human Thrombospondin-2 DuoSet ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human Thrombospondin-2 DuoSet ELISA include:

Species: Human
Sample Types: Amniotic Fluid

S Hong et al. (2022-01-01), Antibody microarray analysis of amniotic fluid proteomes in women with cervical insufficiency and short cervix, and their association with pregnancy latency length PLoS ONE, 17(2):e0263586.
PMID: 35130326
Species: Human
Sample Types: Interstitial Fluid

J Zhang et al. (2017-01-01), In-depth proteomic analysis of tissue interstitial fluid for hepatocellular carcinoma serum biomarker discovery Br. J. Cancer, 117(11):1676-1684.
PMID: 29024941

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Human Thrombospondin-2 DuoSet ELISA

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 07/06/2021

we used this kit to quantify human TSP2 in serum and plasma samples. Did not work and all are below the detection limit.

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Product Documents for Human Thrombospondin-2 DuoSet ELISA

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Human Thrombospondin-2 DuoSet ELISA

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Summary for Human Thrombospondin-2 DuoSet ELISA

Product Specifications for Human Thrombospondin-2 DuoSet ELISA

Kit Contents for Human Thrombospondin-2 DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human Thrombospondin-2 DuoSet ELISA

Preparation and Storage

Background: Thrombospondin-2

Citations for Human Thrombospondin-2 DuoSet ELISA (2)

Customer Reviews for Human Thrombospondin-2 DuoSet ELISA (1)

Customer Images

Human Thrombospondin-2 DuoSet ELISA

Product Documents for Human Thrombospondin-2 DuoSet ELISA

Product Datasheets

COA

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SDS

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