Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Name: Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DPTX30

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DPTX30

Citations (28)

Product Datasheet / COA / SDS

New Version Available - DPTX30B

Discontinued Product

DPTX30 has been discontinued and is replaced by DPTX30B.

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Key Product Details

Assay Length:	5 hours
Sample Type & Volume Required Per Well:	Serum-free Cell Culture Supernates (100 uL), EDTA Plasma (100 uL), Saliva (100 uL)
Sample Types:	Serum-free Cell Culture Supern, EDTA Plasma, Saliva
Sensitivity:	0.116 ng/mL
Assay Range:	0.31 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary:	The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.

View Full Technical Details for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Product Specifications for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human PTX3
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum-free Cell Culture Supernates, EDTA Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (ng/mL)	2.61	7.72	14.1	2.75	7.94	14.5
Standard Deviation	0.01	0.28	0.62	0.17	0.39	0.6
CV%	3.8	3.7	4.4	6.1	5	4.1

Recovery

The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	99	83-106
Serum-free Cell Culture Media (n=4)	101	95-108

Linearity

To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

	Human Pentraxin 3/TSG-14 ELISA Standard Curve
	Human Pentraxin 3/TSG-14 ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Item	Value
Long Name	Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs	5806 (Human); 19288 (Mouse)
Alternate Names	PTX3, Pentaxin-related protein PTX3, Pentraxin 3, TNF alpha-induced protein 5, TNFAIP5, TSG-14, TSG-14pentaxin-related gene, rapidly induced by IL-1 beta, TSG14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, alpha-induced protein 5, pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, pentraxin 3, long, pentraxin-3, pentraxin-related gene, rapidly induced by IL-1 beta, pentraxin-related protein PTX3, tumor necrosis factor, alpha-induced protein 5, tumor necrosis factor-inducible protein TSG-14

Citations for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit (28)

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Citations are publications that use Bio-Techne products. Selected citations for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

J Chivot et al. (2022), PARP Inhibitor Inhibits the Vasculogenic Mimicry through a NF-kappaB-PTX3 Axis Signaling in Breast Cancer Cells International Journal of Molecular Sciences, 23(24).
PMID: 36555812
Species: Human
Sample Types: Plasma

S Rocha et al. (2021), Interleukin 6 (rs1800795) and pentraxin 3 (rs2305619) polymorphisms-association with inflammation and all-cause mortality in end-stage-renal disease patients on dialysis Scientific Reports, 11(1):14768.
PMID: 34285273
Species: Human
Sample Types: Gingival Crevicular Fluid

DN Gheorghe et al. (2021), Non-Surgical Periodontal Therapy Could Improve the Periodontal Inflammatory Status in Patients with Periodontitis and Chronic Hepatitis C Journal of Clinical Medicine, 10(22).
PMID: 34830557
Species: Human
Sample Types: Plasma

C Jin et al. (2021), Elevated plasma pentraxin-3 in polycystic ovary syndrome is associated with hyperandrogenism: a case-control study BMC endocrine disorders, 21(1):240.
PMID: 34856980
Species: Human
Sample Types: Plasma

S Perez-San et al. (2020), Prognostic value of plasma pentraxin 3 levels in patients with septic shock admitted to intensive care PLoS ONE, 15(12):e0243849.
PMID: 33301518
Species: Human
Sample Types: Serum

H Deng et al. (2020), Serum pentraxin 3 as a biomarker of hepatocellular carcinoma in chronic hepatitis B virus infection Sci Rep, 10(1):20276.
PMID: 33219288
Species: Human
Sample Types: Serum

YG Kim et al. (2019), Inevitable isolation and the change of stress markers in hemodialysis patients during the 2015 MERS-CoV outbreak in Korea Sci Rep, 9(1):5676.
PMID: 30952879
Species: Human
Sample Types: Plasma

MJ Valente et al. (2019), Long Pentraxin 3 as a Broader Biomarker for Multiple Risk Factors in End-Stage Renal Disease: Association with All-Cause Mortality Mediators Inflamm., 2019(0):3295725.
PMID: 31316299
Species: Human
Sample Types: Whole Cells

Y Takahashi et al. (2018), Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis Metab. Clin. Exp., 0(0).
PMID: 29410350
Species: Human
Sample Types: Plasma

C Graham et al. (2017), Class II obese and healthy pregnant controls exhibit indistinguishable pro- and anti-inflammatory immune responses to Caesarian section Immun Inflamm Dis, 0(0).
PMID: 28544689
Species: Human
Sample Types: Plasma

S Hamed et al. (2017), Diagnostic value of Pentraxin-3 in patients with sepsis and septic shock in accordance with latest sepsis-3 definitions BMC Infect. Dis., 17(1):554.
PMID: 28793880
Species: Human
Sample Types: Sputum

SJ Thulborn et al. (2017), Investigating the role of pentraxin 3 as a biomarker for bacterial infection in subjects with COPD Int J Chron Obstruct Pulmon Dis, 12(0):1199-1205.
PMID: 28458531
Species: Human
Sample Types: Plasma

Suha Turkmen (2016), The diagnostic value of serum pentraxin 3 levels in pulmonary contusion Am J Emerg Med, 0(0).
PMID: 27955970
Species: Human
Sample Types: Cell Culture Supernates

(2016), Pseudomonas aeruginosa GroEL stimulates production of PTX3 by activating the NF-?B pathway and simultaneously down-regulating microRNA-9 Infect. Immun, 0(0).
PMID: 28031262
Species: Human
Sample Types: Cell Culture Supernates

(2016), Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells Sci Rep, 6(0):37128.
PMID: 27849015
Species: Human
Sample Types: Sputum

Min Jung Kim (2016), Sputum pentraxin 3 as a candidate to assess airway inflammation and remodeling in childhood asthma Medicine (Baltimore), 95(51):e5677.
PMID: 28002338
Species: Human
Sample Types: Serum

Borghini A et al. (2015), Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide. PLoS ONE, 10(6):e0130166.
PMID: 26076019
Species: Human
Sample Types: Plasma

Chu SH et al. (2012), Chemerin and adiponectin contribute reciprocally to metabolic syndrome. PLoS ONE, 7(4):e34710.
PMID: 22509348
Species: Human
Sample Types: Plasma

Outinen TK et al. (2012), Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection. PLoS ONE, 7(2):e31455.
PMID: 22347483
Species: Human
Sample Types: Serum

Luksha L et al. (2012), Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease. PLoS ONE, 7(4):e36056.
PMID: 22563439
Species: Human
Sample Types: Cell Culture Supernates

Zhang J et al. (2012), Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production. PLoS ONE, 7(4):e34965.
PMID: 22529962
Species: Human
Sample Types: Urine

Goodison et al. (2012), A multi-analyte assay for the non-invasive detection of bladder cancer. PLoS ONE, 7(10):e47469.
PMID: 23094052
Species: Human
Sample Types: Plasma

Jylhava J et al. (2011), Pentraxin 3 (PTX3) is associated with cardiovascular risk factors: the Health 2000 Survey. Clin. Exp. Immunol., 164(2):211-7.
PMID: 21391986
Species: Human
Sample Types: Serum

Diamandis EP et al. (2011), Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma. Clin. Cancer Res., 17(8):2395-2399.
PMID: 21257721
Species: Human
Sample Types: Plasma

Huttunen R et al. (2011), High Plasma Level of Long Pentraxin 3 (PTX3) Is Associated with Fatal Disease in Bacteremic Patients: A Prospective Cohort Study. PLoS ONE, 6(3):e17653.
PMID: 21423699
Species: Human
Sample Types: Cell Culture Supernates

Scheel C et al. (2011), Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 145(6):926-40.
PMID: 21663795
Species: Human
Sample Types: Plasma

Miyaki A et al. (2010), Is pentraxin 3 involved in obesity-induced decrease in arterial distensibility? J. Atheroscler. Thromb., 17(3):278-84.
PMID: 20134101
Species: Human
Sample Types: Cell Culture Supernates

May L et al. (2010), Genetic variation in pentraxin (PTX) 3 gene associates with PTX3 production and fertility in women. Biol. Reprod., 82(2):299-304.
PMID: 19846603

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.