Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DPTX30B
Key Product Details
Assay Length
5.0 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 µL), EDTA Plasma (20 µL), Saliva (20 µL)
Sensitivity
0.026 ng/mL
Assay Range
0.313-20 ng/mL (Serum-free Cell Culture Supernates, EDTA Plasma, Saliva)
Product Summary for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
The Quantikine® Human Pentraxin 3/TSG-14 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human Pentraxin 3 in serum-free cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human Pentraxin 3 and antibodies raised against the recombinant protein. Natural human Pentraxin 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human
Pentraxin 3.
Pentraxin 3.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Pentraxin 3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
EDTA Plasma, Saliva, Serum-free Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.12 | 6.03 | 11.6 | 2.05 | 5.86 | 11.4 |
| Standard Deviation | 0.038 | 0.158 | 0.140 | 0.141 | 0.383 | 0.500 |
| CV% | 1.8 | 2.6 | 1.2 | 6.9 | 6.5 | 4.4 |
Recovery for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
The recovery of human Pentraxin 3 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 85-108 |
| Saliva (n=4) | 89 | 79-99 |
| Serum-free Cell Culture Media (n=2) | 109 | 100-120 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Human Pentraxin 3/TSG-14 ELISA Standard Curve
Human Pentraxin 3/TSG-14 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Pentraxin 3/TSG-14
Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.
Long Name
Tumor Necrosis Factor Stimulated Gene 14
Alternate Names
PTX3, TSG-14, TSG14
Gene Symbol
PTX3
Additional Pentraxin 3/TSG-14 Products
Product Documents for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Product Specific Notices for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
For research use only
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