Human FGF-4 DuoSet ELISA

Name: Human FGF-4 DuoSet ELISA
Brand: R&D Systems
SKU: DY235
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY235

Citations (2)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human FGF-4 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human FGF-4 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF-4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human FGF-4 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human FGF-4 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog #DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog #DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)

Stop Solution:2 N H2SO4(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990), or equivalent

Plate Sealers:ELISA Plate Sealers (Catalog #DY992), or equivalent

Scientific Data Examples for Human FGF-4 DuoSet ELISA

	Human FGF-4 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-4

FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation. A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells.

Item	Value
Long Name	Fibroblast Growth Factor 4
Entrez Gene IDs	2249 (Human); 14175 (Mouse); 116499 (Rat)
Alternate Names	FGF-4, FGF4, HBGF-4, HBGF-4Transforming protein KS3, HST-1, HST-1HSTF-1, HSTF1fibroblast growth factor 4 splice isoform, HSTFGF-4, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, K-FGF, KFGF, KS3, fibroblast growth factor 4, heparin secretory transforming protein 1, human stomach cancer, transforming factor from FGF-related oncogene, kaposi sarcoma oncogene, oncogene HST

Citations for Human FGF-4 DuoSet ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGF-4 DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

CH Chou et al. (2019), B-Cell Activating Factor Enhances Hepatocyte-Driven Angiogenesis via B-Cell CLL/Lymphoma 10/Nuclear Factor-KappaB Signaling during Liver Regeneration Int J Mol Sci, 20(20).
PMID: 31658764
Species: Human
Sample Types: Plasma

Sherman JA et al. (2006), Humoral and cellular factors responsible for coronary collateral formation. Am. J. Cardiol., 98(9):1194-7.
PMID: 17056326

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