Human PDGF-AA DuoSet ELISA

Name: Human PDGF-AA DuoSet ELISA
Brand: R&D Systems
SKU: DY221
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY221

Citations (8)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human PDGF-AA DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human PDGF-AA DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PDGF-AA. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human PDGF-AA DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human PDGF-AA DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human PDGF-AA DuoSet ELISA

	Human PDGF-AA ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-AA

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Item	Value
Long Name	Platelet-derived Growth Factor AA
Entrez Gene IDs	5154 (Human); 25266 (Rat)
Alternate Names	PDGF-A, PDGF-AA, PDGF1, PDGFAA

Citations for Human PDGF-AA DuoSet ELISA (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-AA DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

P Szpakowski et al. (2022), Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli Biomedicines, 10(8).
PMID: 35892669
Species: Human
Sample Types: Cell Culture Supernates

H Weisser et al. (2022), Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function Cancer Gene Therapy, 0(0).
PMID: 36114329
Species: Human
Sample Types: Plasma

N Casagrande et al. (2021), In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel International Journal of Molecular Sciences, 22(6).
PMID: 33809553
Species: Human
Sample Types: Nasal Lavage Fluid

S Ravichandr et al. (2021), Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures Science Advances, 7(42):eabi6533.
PMID: 34644111
Species: Human
Sample Types: Cell Culture Supernates

CM Abreu et al. (2021), Rescuing key native traits in cultured dermal papilla cells for human hair regeneration Journal of advanced research, 30(0):103-112.
PMID: 34026290
Species: Human
Sample Types: Serum

LH Lin et al. (2020), Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma Int J Mol Sci, 21(7).
PMID: 32235327
Species: Human
Sample Types: Serum

G Ye et al. (2020), Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis Cell Death Dis, 11(9):775.
PMID: 32943613
Species: Human
Sample Types: Cell Culture Supernates

CY Chen et al. (2019), Induction of nuclear protein-1 by thyroid hormone enhances platelet-derived growth factor A mediated angiogenesis in liver cancer Theranostics, 9(8):2361-2379.
PMID: 31149049

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