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Human CCL2/MCP-1 Quantikine ELISA Kit

Human CCL2/JE/MCP-1 ELISA Cell Culture Supernate/Urine Standard Curve
(3)
Human CCL2/JE/MCP-1 ELISA Serum/Plasma Standard Curve
Human CCL2/JE/MCP-1 ELISA Linearity
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DCP00
SCP00
PDCP00

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Key Product Details

Assay Length: 3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Sensitivity: 10 pg/mL
Assay Range: 31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Human CCL2/MCP-1 Quantikine ELISA Kit Summary: The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.

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Citations for Human CCL2/MCP-1 Quantikine ELISA Kit (227)

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Customer Reviews (8)

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  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 03/15/2022
    Urine was tested at 2-fold, 4-fold, and 8-fold dilutions. All samples were in range at 2-fold dilution, 75% were in range at 4-fold dilution, and 62.5% were in range at 8-fold dilution. All samples at all dilutions had a %Recovery within 80-120% of the reference (2-fold) dilution.We continue to use this kit regularly.
    Human CCL2/MCP-1 Quantikine ELISA Kit DCP00
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/05/2018
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HAEC
    Verified Customer | Posted 11/02/2018
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 05/01/2018
    Human CCL2/MCP-1 Quantikine ELISA Kit DCP00
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Jim Hsiao
    Sample Tested: Primary human lunf fibroblast,Primary human lung fibroblast
    Verified Customer | Posted 12/04/2017
    This result has been published in the PlosONE journal: 10.1371/journal.pone.0058258
    Human CCL2/MCP-1 Quantikine ELISA Kit DCP00
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human tissue
    Verified Customer | Posted 08/18/2017
    Very decent price and perfect customer support
    Human CCL2/MCP-1 Quantikine ELISA Kit DCP00
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: cell culture supernatants
    Verified Customer | Posted 07/24/2017
    cell culture supernatants were diluted 1:5
    Human CCL2/MCP-1 Quantikine ELISA Kit DCP00
  • Human CCL2/MCP-1 Quantikine ELISA Kit
    Name: Anjana Yadav
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 12/14/2016
    MCP-1 concentrations were measured in plasma of HIV+ subjects on HAART and in healthy controls. Blood was collected in EDTA-tubes and plasma separated by centrifugation at 2000rpm for 20 minutes at 20oC. Plasma was diluted 1:2 before use in the assay.Figure Legend for Figure:a) Standard curve for MCP-1 from which values were extrapolated.b) MCP-1 concentrations in plasma of HIV+ and HIV- subjects. Values are expressed as pg/ml and are significantly elevated in HIV+?ART group. Analyses done using non parametric unpaired Mann Whitney U-test.
    Human CCL2/MCP-1 Quantikine ELISA Kit DCP00

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Certificate of Analysis

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.