Human VEGF-C DuoSet ELISA

Name: Human VEGF-C DuoSet ELISA
Brand: R&D Systems
SKU: DY752B
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY752B

Citations (11)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human VEGF-C DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human VEGF-C DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF-C. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human VEGF-C DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human VEGF-C DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human VEGF-C DuoSet ELISA

	Human VEGF-C ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Item	Value
Long Name	Vascular Endothelial Growth Factor C
Entrez Gene IDs	7424 (Human); 22341 (Mouse)
Alternate Names	Flt4 ligand, Flt4-L, VEGF-C, VEGFC, VRPFLT4 ligand DHM, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C

Citations for Human VEGF-C DuoSet ELISA (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human VEGF-C DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

JP Lapuente et al. (2022), Cytokine Profile and Anti-Inflammatory Activity of a Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells (PRS CK STORM) Biomolecules, 12(4).
PMID: 35454123
Species: Human
Sample Types: Serum

Y Ding et al. (2022), VEGF-C serum level is associated with response to bevacizumab maintenance therapy in primary ovarian cancer patients PLoS ONE, 17(6):e0269680.
PMID: 35687576
Species: Human
Sample Types: Cell Culture Supernates

L Cristinzia et al. (2021), IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors Cells, 10(1).
PMID: 33445787
Species: Human
Sample Types: Plasma

G Varricchi et al. (2020), Angiopoietins, Vascular Endothelial Growth Factors and Secretory Phospholipase A2 in Ischemic and Non-Ischemic Heart Failure J Clin Med, 9(6).
PMID: 32575548
Species: Human
Sample Types: Cell Culture Supernates

PD Ndiaye et al. (2019), VEGFC acts as a double-edged sword in renal cell carcinoma aggressiveness Theranostics, 9(3):661-675.
PMID: 30809300
Species: Human
Sample Types: Cell Culture Supernates

G Varricchi et al. (2019), Superantigenic Activation of Human Cardiac Mast Cells Int J Mol Sci, 20(8).
PMID: 31013832
Species: Human
Sample Types: Cell Culture Supernates

J Bourland et al. (2018), Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development Sci Rep, 8(1):13191.
PMID: 30181613
Species: Human
Sample Types: Cell Culture Supernates

M Lupu-Plesu et al. (2017), Effects of proton versus photon irradiation on (lymph)angiogenic, inflammatory, proliferative and anti-tumor immune responses in head and neck squamous cell carcinoma Oncogenesis, 6(7):e354.
PMID: 28671677
Species: Human
Sample Types: Plasma

Kumar N et al. (2017), Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors. J Infect, 74(1):10-21.
PMID: 27717783
Species: Human
Sample Types: Plasma

Kumar N et al. (2016), Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis. PLoS ONE, 11(1):e0146318.
PMID: 26727122
Species: Human
Sample Types: Tissue Culture Supernates

Grepin R et al. (2014), The CXCL7/CXCR1/2 axis is a key driver in the growth of clear cell renal cell carcinoma. Cancer Res, 74(3):873-83.
PMID: 24335961

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