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Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

Human IL-1 beta/IL-1F2 ELISA Cell Culture Supernate Standard Curve
(3)
Human IL-1 beta/IL-1F2 ELISA Serum/Plasma Standard Curve
Human IL-1 beta/IL-1F2 ELISA Linearity
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PDLB50
DLB50
SLB50

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Key Product Details

Assay Length: 3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Sensitivity: 1 pg/mL
Assay Range: 3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary: The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.

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Citations for Human IL-1 beta/IL-1F2 Quantikine ELISA Kit (261)

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Customer Reviews (10)

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  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/05/2018
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 07/13/2018
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 05/31/2018
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Heparin Plasma
    Verified Customer | Posted 05/08/2018
    Among best ELISAs I've worked with. Great consistency lot to lot, high sensitivity, easy to work with small amount of samples (I've diluted samples 1:100 in the past). Appropriate for concentration response curves. The only thing that can be improved is expanding the dynamic range of the assay (highest standard is at 250pg/ml and in our case we sometimes have 20,000pg/ml so need to make significant dilution to fit within the standard curve).
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: CHO Chinese hamster ovary cell line
    Verified Customer | Posted 04/24/2018
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 04/04/2018
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 06/20/2017
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Mike Ehrhardt
    Sample Tested: Serum
    Verified Customer | Posted 04/24/2017
    The Human IL-1beta Quantikine ELISA kit is used for diagnostic use.
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Lamberto Torralba
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 03/15/2017
    Freshly isolated PBMCs from buffy coats stimulated with LPS (1ug/mL) for 3h together with Simvastatin. Total amount of 3 million cells/well. Volume supernatant used: 100uL. Two donors. Great production of IL1B in supernatant. Increased when statins are present as expected. Experiment used as a positive control
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: uman plasma
    Verified Customer | Posted 04/13/2016
    Human IL-1 beta/IL-1F2 Quantikine ELISA Kit DLB50

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FAQs

  • Does the Human IL-1 beta/IL-1F2 Quantikine® ELISA kit (Catalog # DLB50) detect pro or mature IL-1 beta?

    The standard included in the kit is mature recombinant IL-1 beta (p17). This is also the protein used as the immunogen for the antibodies in the kit.

    For these reasons, the kit has been designed to measure mature IL-1 beta. In theory, since the same amino acids present in mature IL-1 beta are also present in pro IL-1 beta (p31), it may be possible for the assay to detect pro IL-1 beta, either fully or partially. However, recombinant pro IL-1 beta is not available for R&D Systems to perform cross-reactivity testing.

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Note: Certificate of Analysis not available for kit components.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.