Human Prolactin Quantikine ELISA Kit

Name: Human Prolactin Quantikine ELISA Kit
Brand: R&D Systems
SKU: DPRL00
Price: 615 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DPRL00

Citations (5)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sample Types:	Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.264 ng/mL
Assay Range:	1.56 - 100 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Human Prolactin Quantikine ELISA Kit Summary:	The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.

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Product Specifications for Human Prolactin Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human Prolactin and natural human macroprolactin
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	16.3	31.8	53.1	15.4	30.6	54.4
Standard Deviation	0.831	1.24	1.79	0.9	1.48	2.94
CV%	5.1	3.9	3.4	5.8	4.8	5.4

Recovery

The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	84-105
Heparin Plasma (n=4)	97	90-104
Serum (n=4)	94	84-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Prolactin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Prolactin Quantikine ELISA Kit

	Human Prolactin ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Prolactin

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Item	Value
Entrez Gene IDs	5617 (Human); 19109 (Mouse)
Alternate Names	PRL, Prolactin

Citations for Human Prolactin Quantikine ELISA Kit (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Prolactin Quantikine ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

Y Fan et al. (2023), Subtle changes in perivascular endometrial mesenchymal stem cells after local endometrial injury in recurrent implantation failure Scientific Reports, 13(1):225.
PMID: 36604485
Species: Human
Sample Types: Plasma

AH Cincotta et al. (2022), Bromocriptine-QR Therapy Reduces Sympathetic Tone and Ameliorates a Pro-Oxidative/Pro-Inflammatory Phenotype in Peripheral Blood Mononuclear Cells and Plasma of Type 2 Diabetes Subjects International Journal of Molecular Sciences, 23(16).
PMID: 36012132
Species: Human
Sample Types: Plasma

M Gabrielson et al. (2020), Hormonal determinants of mammographic density and density change Breast Cancer Res., 22(1):95.
PMID: 32847607
Species: Human
Sample Types: Plasma

M Gabrielson et al. (2020), Hormonal determinants of mammographic density and density change Breast Cancer Res., 22(1):95.
PMID: 32847607
Species: Human
Sample Types: Serum

MN Stein et al. (2017), First-in-human Clinical Trial of Oral ONC201 in Patients with Refractory Solid Tumors Clin. Cancer Res, 0(0).
PMID: 28331050

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Showing 1 - 5 of 5 citations Showing All

Product Documents for Human Prolactin Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.