Human Angiogenin DuoSet ELISA

Name: Human Angiogenin DuoSet ELISA
Brand: R&D Systems
SKU: DY265
Price: 745 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY265

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	7.81 - 500 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Angiogenin DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Angiogenin DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiogenin (ANG). The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Angiogenin DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Angiogenin DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Angiogenin DuoSet ELISA

	Human Angiogenin ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiogenin

Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.

Item	Value
Entrez Gene IDs	283 (Human)
Alternate Names	ALS9, ANG, Angiogenin, EC 3.1.27, EC 3.1.27.-, HEL168, MGC22466, MGC71966, RNASE5RNASE4, RNase 5, Ribonuclease 5, angiogenin, ribonuclease, RNase A family, 5, epididymis luminal protein 168

Citations for Human Angiogenin DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Angiogenin DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

B Wernly et al. (2020-01-01), Anti-CD3 Antibody Treatment Reduces Scar Formation in a Rat Model of Myocardial Infarction Cells, 9(2).
PMID: 31991811
Species: Human
Sample Types: Tissue Homogenates

K Janji? et al. (2017-01-01), L-mimosine and hypoxia can increase angiogenin production in dental pulp-derived cells BMC Oral Health, 17(1):87.
PMID: 28545523
Species: Human
Sample Types: Feces

Dufour V et al. (), Effects of a short-course of amoxicillin/clavulanic acid on systemic and mucosal immunity in healthy adult humans. Int. Immunopharmacol.,5(5):917-28.
PMID: 15778127

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