Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

Name: Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSFB00D

R&D Systems, Inc. a Bio-Techne Brand

Catalog # HSFB00D

Citations (16)

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Key Product Details

Assay Length:	5.5 hours
Sample Type & Volume Required Per Well:	Serum (100 uL), EDTA Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sample Types:	Serum, EDTA Plasma, Citrate Plasma, Urine
Sensitivity:	0.07 pg/mL
Assay Range:	0.313 - 20 pg/mL (Serum, EDTA Plasma, Citrate Plasma, Urine)
Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit Summary:	The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

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Product Specifications for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human FGF basic
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Citrate Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	1.31	5.32	11.4	1.51	5.71	11.1
Standard Deviation	0.1	0.23	0.4	0.12	0.27	0.91
CV%	7.6	4.3	3.5	7.9	4.7	8.2

Recovery

The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Citrate Plasma (n=4)	97	83-118
EDTA Plasma (n=4)	99	85-109
Serum (n=4)	100	88-116
Urine (n=4)	109	100-120

Linearity

To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.

Scientific Data Examples for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

	Human FGF basic ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Item	Value
Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names	Basic fibroblast growth factor, FGF basic, FGF-2, FGF2, FGFBprostatropin, HBGF-2, Prostatropin, bFGF, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

Citations for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit (16)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit include:

Species: Human
Sample Types: Tissue Homogenates

J Sikovanyec et al. (2021-01-01), Angiogenic factors measured in aspirated placental tissue between the 10?+?6 and 18?+?3 weeks of gestation Reproductive biology, 21(4):100572.
PMID: 34798517
Species: Human
Sample Types: Plasma

Briasoulis et al. (2013-01-01), Dose selection trial of metronomic oral vinorelbine monotherapy in patients with metastatic cancer: a hellenic cooperative oncology group clinical translational study. BMC Cancer, 13(1):263.
PMID: 23718900
Species: Human
Sample Types: Plasma

Llovet JM et al. (2012-01-01), Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma. Clin. Cancer Res., 18(8):2290-300.
PMID: 22374331
Species: Human
Sample Types: Plasma

Schirmer SH et al. (2009-01-01), Local cytokine concentrations and oxygen pressure are related to maturation of the collateral circulation in humans. J. Am. Coll. Cardiol., 53(23):2141-7.
PMID: 19497439
Species: Human
Sample Types: Platelet Poor Plasma

Kanter J et al. (2008-01-01), Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates. Clin. Cancer Res., 14(12):3942-7.
PMID: 18559616
Species: Ovine
Sample Types: Serum

Wedgwood S et al. (2007-01-01), Fibroblast growth factor-2 expression is altered in lambs with increased pulmonary blood flow and pulmonary hypertension. Pediatr. Res., 61(1):32-6.
PMID: 17211137
Species: Mouse
Sample Types: Cell Culture Supernates

Weiner L et al. (2007-01-01), Dedicated epithelial recipient cells determine pigmentation patterns. Cell, 130(5):932-42.
PMID: 17803914
Species: Human
Sample Types: Serum

Anagnostopoulos A et al. (2007-01-01), Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity. Br. J. Haematol., 137(6):560-8.
PMID: 17451406
Species: Human
Sample Types: Plasma

Schneider P et al. (2007-01-01), Endostatin variations in childhood acute lymphoblastic leukaemia--comparison with basic fibroblast growth factor and vascular endothelial growth factor. Leuk. Res., 31(5):629-38.
PMID: 17011029
Species: Human
Sample Types: Peritoneal Fluid

Bourlev V et al. (2006-01-01), Elevated levels of fibroblast growth factor-2 in serum from women with endometriosis. Am. J. Obstet. Gynecol., 194(3):755-9.
PMID: 16522409
Species: Human
Sample Types: Urine

Kulke MH et al. (2006-01-01), Phase II study of recombinant human endostatin in patients with advanced neuroendocrine tumors. J. Clin. Oncol., 24(22):3555-61.
PMID: 16877721
Species: Human
Sample Types: Plasma

Mross K et al. (2005-01-01), Phase I clinical and pharmacokinetic study of PTK/ZK, a multiple VEGF receptor inhibitor, in patients with liver metastases from solid tumours. Eur. J. Cancer, 41(9):1291-9.
PMID: 15939265
Species: Human
Sample Types: Cell Culture Supernates

Lacoste E et al. (2003-01-01), Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J. Periodontol., 74(10):1498-507.
PMID: 14653397
Species: Mouse
Sample Types: Serum

Huang SF et al. (2002-01-01), Inhibition of growth and metastasis of orthotopic human prostate cancer in athymic mice by combination therapy with pegylated interferon-alpha-2b and docetaxel. Cancer Res., 62(20):5720-6.
PMID: 12384530
Species: Bovine
Sample Types: Serum

Esner M et al. (2002-01-01), Targeted disruption of fibroblast growth factor receptor-1 blocks maturation of visceral endoderm and cavitation in mouse embryoid bodies. ÎÎ¶)&Š4wJ]¾b.ŠKd•ÿ¶fäMò&¨k³, 2002;46(6):817-25.
PMID: 12382948
Species: Canine
Sample Types: Urine, Whole Cells

Allen DK et al. (1996-01-01), High urine concentrations of basic fibroblast growth factor in dogs with bladder cancer. J. Vet. Intern. Med., 10(4):231-4.
PMID: 8819047

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