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Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

Human IL-1 beta/IL-1F2 ELISA Standard Curve
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Human IL-1 beta/IL-1F2 High Sensitivity Standard Curve
Human IL-1 beta/IL-1F2 ELISA Linearity
Human IL-1 beta/IL-1F2 High Sensitivity ELISA Linearity
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PHSLB00D
SSLB00D
HSLB00D

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Key Product Details

Assay Length: 4.0 hours
Sample Type & Volume Required Per Well: Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (10 uL)
Sample Types: Serum, EDTA Plasma, Heparin Plasma, Saliva
Sensitivity: 0.063 pg/mL
Assay Range: 0.125 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Saliva)
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary: The Quantikine HS Human IL-1 beta Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1 beta levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum, plasma, and saliva.

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Citations for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit (59)

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Customer Reviews (7)

4.7 out of 5
7 customer ratings
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  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/13/2020
    Standards all the wells in 1 and 2.R&D QC Low in well 3A and R&D QC High in well 12H.Rest are serum samples.
    Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Serum HSLB00D
  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 11/09/2020
    Good standard curve signal and clear protocol.
    Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Serum HSLB00D
  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/27/2020
    Great assay with very easy to follow protocol.
    Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Serum HSLB00D
  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 01/30/2020
    Easy to use, good results!
    Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Serum HSLB00D
  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Purified Standard
    Verified Customer | Posted 01/30/2020
    Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Serum HSLB00D
  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 01/20/2020
  • Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Peripheral blood neutrophils
    Verified Customer | Posted 08/04/2017
    100uL of sample (neat for my experiments) is required per well. Easy to follow instructions.
    Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Serum HSLB00D

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.