Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 4.0 hours |
Sample Type & Volume Required Per Well: | Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (10 uL) |
Sample Types: | Serum, EDTA Plasma, Heparin Plasma, Saliva |
Sensitivity: | 0.063 pg/mL |
Assay Range: | 0.125 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Saliva) |
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary: | The Quantikine HS Human IL-1 beta Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1 beta levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum, plasma, and saliva. |
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Citations for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit (70)
Citations are publications that use Bio-Techne products. Selected citations for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit include:
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Species: Human
Sample Types: Plasma
K Wolk et al. (2023-01-01), Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment International Journal of Molecular Sciences, 24(2).
PMID: 36674793 -
Species: Human
Sample Types: Cell Culture Supernates
SM Zarza et al. (2023-01-01), Infection and Persistence of Coxiella burnetii Clinical Isolate in the Placental Environment International Journal of Molecular Sciences, 24(2).
PMID: 36674725 -
Species: Human
Sample Types: Plasma
P Binou et al. (2022-01-01), Wheat Biscuits Enriched with Plant-Based Protein Contribute to Weight Loss and Beneficial Metabolic Effects in Subjects with Overweight/Obesity Nutrients, 14(12).
PMID: 35745249 -
Species: Human
Sample Types: Plasma
MS Simon et al. (2022-01-01), A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol PLoS ONE, 17(7):e0271793.
PMID: 35862413 -
Species: Human
Sample Types: Serum
N Kobayashi et al. (2022-01-01), Suppressors of Cytokine Signaling Are Decreased in Major Depressive Disorder Patients Journal of personalized medicine, 12(7).
PMID: 35887537 -
Species: Human
Sample Types: Plasma
M Liu et al. (2021-01-01), Macrophage migration inhibitory factor may play a protective role in osteoarthritis Arthritis Research Therapy, 2021;23(1):59.
PMID: 33610191 -
Species: Human
Sample Types: Serum
A Diaz et al. (2021-01-01), Effects of brief stress management interventions on distress and leukocyte nuclear factor kappa B expression during primary treatment for breast cancer: A randomized trial Psychoneuroendocrinology, 126(0):105163.
PMID: 33611132 -
Species: Human
Sample Types: Plasma
MM Leite et al. (2021-01-01), Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus Experimental gerontology, 150(0):111389.
PMID: 33957262 -
Species: Human
Sample Types: Plasma
SM Ng et al. (2021-01-01), Impact of mind-body intervention on proinflammatory cytokines interleukin 6 and 1beta: A three-arm randomized controlled trial for persons with sleep disturbance and depression Brain, Behavior, and Immunity, 99(0):166-176.
PMID: 34634445 -
Species: Human
Sample Types: Plasma
M Wiecek et al. (2021-01-01), Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men Biomolecules, 11(7).
PMID: 34356664 -
Species: Human
Sample Types: Plasma
MME de Jong et al. (2021-01-01), The multiple myeloma microenvironment is defined by an inflammatory stromal cell landscape Nature Immunology, 22(6):769-780.
PMID: 34017122 -
Species: Human
Sample Types: Cell Culture Supernates
Y Du et al. (2021-01-01), Nicotine regulates autophagy of human periodontal ligament cells through alpha7 nAchR that promotes secretion of inflammatory factors IL-1beta and IL-8 BMC Oral Health, 21(1):560.
PMID: 34732192 -
Species: Human
Sample Types: Cell Culture Supernates
M Yamaya et al. (2021-01-01), The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells Virus research, 304(0):198548.
PMID: 34425162 -
Species: Human
Sample Types: Serum
J Doll et al. (2020-01-01), LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy J Orthop, 17(0):208-214.
PMID: 31889743 -
Species: Human
Sample Types: Plasma
SM Ng et al. (2020-01-01), Trust as a mediator in the relationship between childhood sexual abuse and IL-6 level in adulthood PLoS ONE, 15(5):e0232932.
PMID: 32413063 -
Species: Human
Sample Types: Serum
SR Kim et al. (2020-01-01), SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease Nat Commun, 11(1):2127.
PMID: 32358544 -
Species: Human
Sample Types: Plasma
KA Davies et al. (2020-01-01), Interferon and anti-TNF therapies differentially modulate amygdala reactivity which predicts associated bidirectional changes in depressive symptoms Mol. Psychiatry, 0(0).
PMID: 32457424 -
Species: Human
Sample Types: Urine
S Ma et al. (2020-01-01), Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study Biomedicines, 8(3).
PMID: 32143279 -
Species: Human
Sample Types: Serum
MH Hanindita et al. (2020-01-01), Comparison of two lipid emulsions on interleukin-1&beta, interleukin-8 and fatty acid composition in infants post gastrointestinal surgery:�a randomized trial F1000Research, 9(0):1168.
PMID: 33299555 -
Species: Human
Sample Types: Serum
NB Søndertoft et al. (2020-01-01), The intestinal microbiome is a co-determinant of the postprandial plasma glucose response PLoS ONE, 15(9):e0238648.
PMID: 32947608 -
Species: Human
Sample Types: Serum
I Conejero et al. (2019-01-01), Association between baseline pro-inflammatory cytokines and brain activation during social exclusion in patients with vulnerability to suicide and depressive disorder Psychoneuroendocrinology, 99(0):236-242.
PMID: 30326381 -
Species: Human
Sample Types: Serum
SR Chaudhry et al. (2019-01-01), Inter-ictal assay of peripheral circulating inflammatory mediators in migraine patients under adjunctive cervical non-invasive vagus nerve stimulation (nVNS): A proof-of-concept study Brain Stimul, 0(0).
PMID: 30745260 -
Species: Human
Sample Types: Plasma
L Rudzki et al. (2019-01-01), Probiotic Lactobacillus Plantarum 299v decreases kynurenine concentration and improves cognitive functions in patients with major depression: A double-blind, randomized, placebo controlled study Psychoneuroendocrinology, 100(0):213-222.
PMID: 30388595 -
Species: Human
Sample Types: Plasma
G Gagno et al. (2019-01-01), Galectin 3 and Galectin 3 Binding Protein Improve the Risk Stratification after Myocardial Infarction J Clin Med, 8(5).
PMID: 31035456 -
Species: Human
Sample Types: Tissue Culture Supernates
X Jiang et al. (2019-01-01), Inflammasome-Driven Interleukin-1α and�Interleukin-1? Production in Atherosclerotic Plaques Relates to Hyperlipidemia and Plaque Complexity JACC Basic Transl Sci, 4(3):304-317.
PMID: 31312755 -
Species: Human
Sample Types: Serum
N Aziz et al. (2019-01-01), Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 20(1):33.
PMID: 31521107 -
TH Hung et al. (2019-01-01), Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis Mediators Inflamm., 2019(0):8687120.
PMID: 31885501 -
Species: Human
Sample Types: Cell Lysates
M Hollborn et al. (2018-01-01), Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression Mol. Vis., 24(0):518-535.
PMID: 30090015 -
Species: Human
Sample Types: Plasma
M Le Dinh et al. (2018-01-01), Prolonged mechanical ventilation worsens sepsis-induced diaphragmatic dysfunction in the rat PLoS ONE, 13(8):e0200429.
PMID: 30067847 -
Species: Human
Sample Types: Plasma
G Bosco et al. (2018-01-01), Effects of the Ketogenic diet in overweight divers breathing Enriched Air Nitrox Sci Rep, 8(1):2655.
PMID: 29422679 -
Species: Human
Sample Types: Plasma
CK Wu et al. (2018-01-01), Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients J Clin Med, 7(11).
PMID: 30413105 -
Species: Human
Sample Types: Plasma
AC Passaroni et al. (2018-01-01), Hemolysis and Inflammatory Response to Extracorporeal Circulation during On-Pump CABG: Comparison between Roller and Centrifugal Pump Systems Braz J Cardiovasc Surg, 33(1):64-71.
PMID: 29617504 -
Species: Human
Sample Types: Serum
N Sarabon et al. (2018-01-01), Acute effect of different concentrations of cayenne pepper cataplasm on sensory-motor functions and serum levels of inflammation-related biomarkers in healthy subjects Eur J Transl Myol, 28(1):7333.
PMID: 29686821 -
Species: Human
Sample Types: Serum
TD Trippel et al. (2017-01-01), Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study ESC Heart Fail, 4(1):56-65.
PMID: 28217313 -
Species: Human
Sample Types: Plasma
E Peyster et al. (2017-01-01), Inflammation and Arterial Stiffness in Chronic Kidney Disease: Findings From the CRIC Study Am. J. Hypertens., 30(4):400-408.
PMID: 28391349 -
Species: Human
Sample Types: Serum
N Fuke et al. (2017-01-01), Effect of combined consumption of Lactobacillus brevis KB290 and ?-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial Int J Food Sci Nutr, 0(0):1-14.
PMID: 28391736 -
Species: Human
Sample Types: Plasma
A Aleksova et al. (2017-01-01), Interleukin-1? levels predict long-term mortality and need for heart transplantation in ambulatory patients affected by idiopathic dilated cardiomyopathy Oncotarget, 8(15):25131-25140.
PMID: 28212578 -
Species: Human
Sample Types: Plasma
HY Tan et al. (2016-01-01), Aberrant Inflammasome Activation Characterizes Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome J Immunol, 0(0).
PMID: 27076678 -
Species: Human
Sample Types: Plasma
Kim H et al. (2015-01-01), Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses. PLoS ONE, 10(9):e0137567.
PMID: 26352938 -
Species: Human
Sample Types: Plasma
Gupta J et al. (2015-01-01), Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study. PLoS ONE, 10(4):e0124772.
PMID: 25909952 -
Species: Human
Sample Types: Cell Culture Supernates
Proenca-Ferreira R et al. (2014-01-01), Endothelial activation by platelets from sickle cell anemia patients. PLoS ONE, 9(2):e89012.
PMID: 24551209 -
Species: Human
Sample Types: Serum
Zhou X et al. (2014-01-01), High-salt intake induced visceral adipose tissue hypoxia and its association with circulating monocyte subsets in humans. Obesity (Silver Spring), 22(6):1470-6.
PMID: 24493236 -
Species: Human
Sample Types: Sputum
Singh R et al. (2014-01-01), Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. Respir Res, 15(0):114.
PMID: 25218165 -
Species: Human
Sample Types: Plasma
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PMID: 24470107 -
Species: Human
Sample Types: Plasma
Wing M et al. (2014-01-01), Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study. Obesity (Silver Spring), 22(5):1359-66.
PMID: 24415732 -
Species: Human
Sample Types: Serum
Salsano et al. (2013-01-01), An autoinflammatory neurological disease due to interleukin 6 hypersecretion. J Neuroinflammation, 10(0):29.
PMID: 23432807 -
Species: Human
Sample Types: Plasma
Beghe B et al. (2013-01-01), Echocardiography, spirometry, and systemic acute-phase inflammatory proteins in smokers with COPD or CHF: an observational study. PLoS ONE, 8(11):e80166.
PMID: 24244639 -
Species: Human
Sample Types: Plasma
Ngan D et al. (2012-01-01), The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis. BMC Pulm Med, 12(0):3.
PMID: 22333132 -
Species: Human
Sample Types: Plasma
Pournot H et al. (2011-01-01), Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise. PLoS ONE, 6(7):e22748.
PMID: 21829501 -
Species: Human
Sample Types: Plasma
Schneider MP et al. (2011-01-01), Folic acid treatment normalizes NOS-dependence of vascular tone in the metabolic syndrome. Obesity (Silver Spring), 19(5):960-7.
PMID: 20864946 -
Species: Human
Sample Types: Cell Culture Supernates
Verma S et al. (2011-01-01), Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes. J. Gen. Virol., 92(0):507-15.
PMID: 21106803 -
Species: Human
Sample Types: Plasma
Ruotsalainen E et al. (2010-01-01), Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects. Atherosclerosis, 210(2):536-41.
PMID: 20031127 -
Species: Human
Sample Types: Plasma
Backstrom E et al. (2010-01-01), Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia. Am. J. Respir. Cell Mol. Biol., 42(2):149-60.
PMID: 19411613 -
Species: Human
Sample Types: Serum
Hatzistilianou M et al. (2010-01-01), Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia. Inflamm. Res., 59(5):339-47.
PMID: 19806318 -
Species: Human
Sample Types: Serum
Antonelli A et al. (2010-01-01), Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis. J. Interferon Cytokine Res., 30(11):835-42.
PMID: 20929277 -
Species: Human
Sample Types: Serum
Singh S et al. (2009-01-01), Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett., 283(1):29-35.
PMID: 19375853 -
Species: Human
Sample Types: Serum
Sepah SC et al. (2009-01-01), Positive affect and inflammation during radiation treatment for breast and prostate cancer. Brain Behav. Immun., 23(8):1068-72.
PMID: 19559785 -
Species: Human
Sample Types: Plasma
Lachmann HJ et al. (2009-01-01), In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes. J. Exp. Med., 206(5):1029-36.
PMID: 19364880 -
Species: Human
Sample Types: Serum
Venkateswaran RV et al. (2009-01-01), The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy. Transplantation, 88(4):582-8.
PMID: 19696643 -
Species: Human
Sample Types: Plasma
Poulsen TD et al. (2009-01-01), Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery. J. Cardiothorac. Vasc. Anesth., 23(3):316-23.
PMID: 18948032 -
Species: Human
Sample Types: Saliva
Tobon-Arroyave S et al. (2008-01-01), Correlation between salivary IL-1beta levels and periodontal clinical status. Arch Oral Biol, 53(4):346-52.
PMID: 18155182 -
Species: Human
Sample Types: Serum
Richey LM et al. (2007-01-01), Defining cancer cachexia in head and neck squamous cell carcinoma. Clin. Cancer Res., 13(22):6561-7.
PMID: 18006755 -
Species: Human
Sample Types: Tissue Secretion
Gouin JP et al. (2007-01-01), The influence of anger expression on wound healing. Brain Behav. Immun., 22(5):699-708.
PMID: 18078737 -
Species: Human
Sample Types: Plasma
Yamanaka K et al. (2006-01-01), Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma. Clin. Cancer Res., 12(2):376-82.
PMID: 16428475 -
Species: Human
Sample Types: Serum
Miranda LA et al. (2005-01-01), Increased interleukin-18 in patients with juvenile idiopathic arthritis and early attachment loss. J. Periodontol., 76(1):75-82.
PMID: 15830640 -
Species: Human
Sample Types: Exhaled Breath Condensate (EBC)
Garey KW et al. (2004-01-01), Markers of inflammation in exhaled breath condensate of young healthy smokers. Chest, 125(1):22-6.
PMID: 14718416 -
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Serum
Geisbert TW et al. (2003-01-01), Pathogenesis of Ebola hemorrhagic fever in cynomolgus macaques: evidence that dendritic cells are early and sustained targets of infection. Am. J. Pathol., 163(6):2347-70.
PMID: 14633608 -
Species: Human
Sample Types: Serum
Smith DE et al. (2003-01-01), The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action. Immunity, 18(1):87-96.
PMID: 12530978 -
Species: Human
Sample Types: Serum
Schulz O et al. (2002-01-01), Impact of an exercise-induced increase in cardiac troponin I in chronic heart failure secondary to ischemic or idiopathic dilated cardiomyopathy. Am. J. Cardiol., 90(5):547-50.
PMID: 12208423 -
Species: Primate
Sample Types: Serum
Yang C et al. (1999-01-01), Cytokine production in rhesus monkeys infected with Plasmodium coatneyi. Am. J. Trop. Med. Hyg., 61(2):226-9.
PMID: 10463671
There are no citations that match your criteria.
Customer Reviews for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit (7)
Customer Images
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Sample Tested: SerumVerified Customer | Posted 11/13/2020Standards all the wells in 1 and 2.R&D QC Low in well 3A and R&D QC High in well 12H.Rest are serum samples.
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Sample Tested: EDTA PlasmaVerified Customer | Posted 11/09/2020Good standard curve signal and clear protocol.
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Sample Tested: SerumVerified Customer | Posted 10/27/2020Great assay with very easy to follow protocol.
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Sample Tested: PlasmaVerified Customer | Posted 01/30/2020Easy to use, good results!
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Sample Tested: Purified StandardVerified Customer | Posted 01/30/2020
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Sample Tested: Serum and PlasmaVerified Customer | Posted 01/20/2020
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Sample Tested: Peripheral blood neutrophilsVerified Customer | Posted 08/04/2017100uL of sample (neat for my experiments) is required per well. Easy to follow instructions.
There are no reviews that match your criteria.
FAQs for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
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Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
Product Documents for Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.