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Human Serpin E1/PAI-1 Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Length: | 4.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), CTAD Plasma (50 uL) |
| Sample Types: | Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma |
| Sensitivity: | 0.142 ng/mL |
| Assay Range: | 0.312 - 20 ng/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma) |
| Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary: | The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1. |
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Human Serpin E1/PAI-1 ELISA Standard Curve |
Citations for Human Serpin E1/PAI-1 Quantikine ELISA Kit (30)
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Citations are publications that use Bio-Techne products. Selected citations for Human Serpin E1/PAI-1 Quantikine ELISA Kit include:
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Species: Human
Sample Types: Plasma
C Voukalis et al. (2021), Effects of antithrombotic drugs on the prothrombotic state in patients with atrial fibrillation: The west Birmingham atrial fibrillation project Thrombosis Research, 200(0):149-155.
PMID: 33610887 -
Species: Human
Sample Types: Cell Culture Supernates
L Peng et al. (2020), Expression of trophoblast derived prostaglandin E2 receptor 2 (EP2) is reduced in patients with recurrent miscarriage and EP2 regulates cell proliferation and expression of inflammatory cytokines J Reprod Immunol, 142(0):103210.
PMID: 33011635 -
Species: Human
Sample Types: Serum
JN Justice et al. (2019), Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study EBioMedicine, 0(0).
PMID: 30616998 -
Species: Human
Sample Types: Cell Culture Supernates
Y Ye et al. (2019), Anti ?-enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages EBioMedicine, 0(0).
PMID: 30827932 -
Species: Human
Sample Types: Cell Culture Supernates
Y Ye et al. (2018), Prostaglandin E2 receptor 3 (EP3) signaling is induced in placentas with unexplained recurrent pregnancy losses (uRPL) Endocr Connect, 0(0).
PMID: 29700097 -
Species: Human
Sample Types: Plasma
M Jakubowski et al. (2017), Platelet Carbonic Anhydrase II, a Forgotten Enzyme, May Be Responsible for Aspirin Resistance Oxid Med Cell Longev, 2017(0):3132063.
PMID: 29090039 -
Species: Human
Sample Types: Serum
S Chen et al. (2017), Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders Sci Rep, 7(1):6871.
PMID: 28761093 -
Species: Human
Sample Types: Cell Lysates
(2016), MAP3K19 Is a Novel Regulator of TGF-? Signaling That Impacts Bleomycin-Induced Lung Injury and Pulmonary Fibrosis PLoS ONE, 11(5):e0154874.
PMID: 27144281 -
Species: Human
Sample Types: Serum
(2016), Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies Sci Rep, 6(0):30464.
PMID: 27456456 -
Species: Human
Sample Types: Plasma
(2016), Circadian misalignment increases cardiovascular disease risk factors in humans Proc. Natl. Acad. Sci. U.S.A., 113(10):E1402-11.
PMID: 26858430 -
Species: Human
Sample Types: Cell Culture Supernates
Yamashita A et al. (2014), Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage. PLoS ONE, 9(1):e86426.
PMID: 24466087 -
Species: Human
Sample Types: Whole Cells
Chang M et al. (2014), p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells. PLoS ONE, 9(12):e114446.
PMID: 25517907 -
Species: Human
Sample Types: Cell Culture Supernates
Santhekadur P et al. (2014), Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling. FEBS Open Bio, 4(0):353-61.
PMID: 24918049 -
Species: Human
Sample Types: Plasma
Masia M et al. (2014), Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients. PLoS ONE, 9(8):e105442.
PMID: 25133669 -
Species: Human
Sample Types: Serum
Portilla J et al. (2014), Vitamin D insufficiency and subclinical atherosclerosis in non-diabetic males living with HIV. J Int AIDS Soc, 17(0):18945.
PMID: 24836607 -
Species: Human
Sample Types: Plasma
Hernandez S et al. (2014), Characterization of circulating and endothelial progenitor cells in patients with extreme-duration type 1 diabetes. Diabetes Care, 37(8):2193-201.
PMID: 24780357 -
Species: Human
Sample Types: Cell Culture Supernates
Fujimura K et al. (2014), Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice. PLoS ONE, 9(4):e94373.
PMID: 24747517 -
Species: Human
Sample Types: Cell Culture Supernates
(2013), Dipyridamole decreases inflammatory metalloproteinase-9 expression and release by human monocytes. Thromb Haemost, 109(2):280-9.
PMID: 23238437 -
Species: Human
Sample Types: Plasma
(2012), Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis. BMC Infect Dis, 12(0):144.
PMID: 22731696 -
Species: Human
Sample Types: Cell Lysates
Djafarzadeh R et al. (2012), Recombinant GPI-anchored TIMP-1 stimulates growth and migration of peritoneal mesothelial cells. PLoS ONE, 7(4):e33963.
PMID: 22558080 -
Species: Human
Sample Types: Plasma
Lira FS et al. (2011), Exercise training improves sleep pattern and metabolic profile in elderly people in a time-dependent manner. Lipids Health Dis, 10(0):113.
PMID: 21733182 -
Species: Human
Sample Types: Cell Culture Supernates
Zhang P et al. (2011), Endothelial differentiation of adipose-derived stem cells from elderly patients with cardiovascular disease. Stem Cells Dev., 20(6):977-88.
PMID: 20879833 -
Species: Human
Sample Types: Cell Culture Supernates
Chang W et al. (2010), SPARC suppresses apoptosis of idiopathic pulmonary fibrosis fibroblasts through constitutive activation of beta-catenin. J. Biol. Chem., 285(11):8196-206.
PMID: 20061390 -
Species: Human
Sample Types: Cell Culture Supernates
Ma Z et al. (2010), Cyclic adenosine monophosphate induces plasminogen activator inhibitor-1 expression in human mast cells. Biochem. Biophys. Res. Commun., 400(4):569-74.
PMID: 20816667 -
Species: Human
Sample Types: Plasma
Patel N et al. (2010), Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD). J. Biol. Chem., 285(22):16713-22.
PMID: 20351105 -
Species: Human
Sample Types: Cell Culture Supernates
Gomez D et al. (2009), Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 218(1):131-42.
PMID: 19224541 -
Species: Human
Sample Types: Cell Culture Supernates
Tkachuk N et al. (2008), Urokinase induces survival or pro-apoptotic signals in human mesangial cells depending on the apoptotic stimulus. Biochem. J., 415(2):265-73.
PMID: 18564064 -
Species: Human
Sample Types: Plasma
Sharma K et al. (2008), Adiponectin regulates albuminuria and podocyte function in mice. J. Clin. Invest., 118(5):1645-56.
PMID: 18431508 -
Species: Human
Sample Types: Cell Culture Supernates
Huang MC et al. (0), Human CD4- 8- T cells are a distinctive immunoregulatory subset. FASEB 24(7):2558-66.
PMID: 20154266 -
Species: Human
Sample Types: Cell Culture Supernates
Rolandsson Enes S et al. (0), MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC. Sci Re6(0):29160.
PMID: 27381039
There are no citations that match your criteria.
Customer Reviews for Human Serpin E1/PAI-1 Quantikine ELISA Kit (4)
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Sample Tested: PlasmaVerified Customer | Posted 12/24/2020Standard curve in duplicate in Columns 1 and 2
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Sample Tested: EDTA PlasmaVerified Customer | Posted 12/24/2020Comprehensive and accurate assay that was easy to execute
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Sample Tested: PlasmaVerified Customer | Posted 12/16/2020Great assay with a very clear protocol. Produced results as expected.
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Sample Tested: cell culture supernatantsVerified Customer | Posted 07/20/2017used at 1:2 dilution for cell culture supernatants
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Product Documents for Human Serpin E1/PAI-1 Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
