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Human Serpin E1/PAI-1 Quantikine ELISA Kit

R&D Systems, Inc. a Bio-Techne Brand

Human Serpin E1/PAI-1 ELISA Standard Curve
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Human Serpin E1/PAI-1 ELISA Linearity
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DSE100

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Setting the standard in quality research reagents

Key Product Details

Assay Length: 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), CTAD Plasma (50 uL)
Sample Types: Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma
Sensitivity: 0.142 ng/mL
Assay Range: 0.312 - 20 ng/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma)
Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary: The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.

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Customer Reviews for Human Serpin E1/PAI-1 Quantikine ELISA Kit (4)

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  • Human Serpin E1/PAI-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 12/24/2020
    Standard curve in duplicate in Columns 1 and 2
    Human Serpin E1/PAI-1 Quantikine ELISA Kit Serum DSE100
  • Human Serpin E1/PAI-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 12/24/2020
    Comprehensive and accurate assay that was easy to execute
    Human Serpin E1/PAI-1 Quantikine ELISA Kit Serum DSE100
  • Human Serpin E1/PAI-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 12/16/2020
    Great assay with a very clear protocol. Produced results as expected.
    Human Serpin E1/PAI-1 Quantikine ELISA Kit Serum DSE100
  • Human Serpin E1/PAI-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: cell culture supernatants
    Verified Customer | Posted 07/20/2017
    used at 1:2 dilution for cell culture supernatants
    Human Serpin E1/PAI-1 Quantikine ELISA Kit Serum DSE100

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Product Documents for Human Serpin E1/PAI-1 Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.