Human GM-CSF Quantikine HS ELISA Kit

Name: Human GM-CSF Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSGM0
Price: 655 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # HSGM0

Citations (11)

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Key Product Details

Assay Length:	6.5 hours
Sample Type & Volume Required Per Well:	Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (200 uL)
Sample Types:	Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.26 pg/mL
Assay Range:	1 - 64 pg/mL (Serum, EDTA Plasma, Heparin Plasma) 0.5 - 32 pg/mL (Urine)
Human GM-CSF Quantikine HS ELISA Kit Summary:	The Quantikine HS Human GM-CSF Immunoassay is a 6.5 hour solid phase ELISA designed to measure human GM-CSF in serum, plasma, and urine. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GM-CSF.

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Product Specifications for Human GM-CSF Quantikine HS ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human GM-CSF
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	18	18	18
Mean (pg/mL)	3.68	22	44.6	2.8	16.2	33.2
Standard Deviation	0.35	1.07	1.76	0.66	2.47	4.6
CV%	9.5	4.9	4	23.5	15.3	13.9

Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	2.69	7.05	24.8	3.77	14.3	27.4
Standard Deviation	0.14	0.33	0.73	0.37	1.17	1.85
CV%	5.2	4.7	2.9	9.8	8.2	6.8

Recovery

The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	114	88-140
Heparin Plasma (n=4)	109	91-122
Serum (n=4)	109	94-128
Urine (n=5)	102	95-116

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human GM-CSF Quantikine HS ELISA Kit

	Human GM-CSF ELISA Urine Assay
	Human GM-CSF ELISA Serum/Plasma Assay

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.

Item	Value
Long Name	Granulocyte Macrophage Growth Factor
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names	CSF, CSF-2, CSF2, Colony-stimulating factor, GM-CSF, GMCSF, GMCSFgranulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, Molgramostim, Sargramostim, colony stimulating factor 2 (granulocyte-macrophage), granulocyte-macrophage colony stimulating factor, molgramostin

Citations for Human GM-CSF Quantikine HS ELISA Kit (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human GM-CSF Quantikine HS ELISA Kit include:

Species: Human
Sample Types: Plasma

Y Luo et al. (2018), Decreased Count and Dysfunction of Circulating EPCs in Postmenopausal Hypercholesterolemic Females via Reducing NO Production Stem Cells Int, 2018(0):2543847.
PMID: 29760721
Species: Human
Sample Types: Plasma

K Tanisawa et al. (2018), Effects of Ingestion of Different Amounts of Carbohydrate after Endurance Exercise on Circulating Cytokines and Markers of Neutrophil Activation Antioxidants (Basel), 7(4).
PMID: 29614808
Species: Mouse
Sample Types: Plasma

F Reggiani et al. (2017), Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression Cancer Res., 0(0).
PMID: 28754674
Species: Human
Sample Types: Serum

SP Buchbinder et al. (2017), Immunogenicity of a novel Clade B HIV-1 vaccine combination: Results of phase 1 randomized placebo controlled trial of an HIV-1 GM-CSF-expressing DNA prime with a modified vaccinia Ankara vaccine boost in healthy HIV-1 uninfected adults PLoS ONE, 12(7):e0179597.
PMID: 28727817
Species: Human
Sample Types: Serum

Lipson E et al. (2015), Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting. J Transl Med, 13(0):214.
PMID: 26143264
Species: Human
Sample Types: BALF

Paine R et al. (2012), A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury. Crit. Care Med., 40(1):90-7.
PMID: 21926600
Species: Human
Sample Types: Serum

Emens LA et al. (2009), Timed sequential treatment with cyclophosphamide, doxorubicin, and an allogeneic granulocyte-macrophage colony-stimulating factor-secreting breast tumor vaccine: a chemotherapy dose-ranging factorial study of safety and immune activation. J. Clin. Oncol., 27(35):5911-8.
PMID: 19805669
Species: Human
Sample Types: Plasma

Grann VR et al. (2008), Duffy (Fy), DARC, and neutropenia among women from the United States, Europe and the Caribbean. Br. J. Haematol., 143(2):288-93.
PMID: 18710383
Species: Human
Sample Types: Plasma

Grann VR et al. (2008), Neutropenia in 6 ethnic groups from the Caribbean and the U.S. Cancer, 113(4):854-60.
PMID: 18543314
Species: Human
Sample Types: Serum

Rafat N et al. (2007), Increased circulating endothelial progenitor cells in septic patients: correlation with survival. Crit. Care Med., 35(7):1677-84.
PMID: 17522579
Species: Ovine
Sample Types: BALF

Kramer BW et al. (2007), Endotoxin-induced maturation of monocytes in preterm fetal sheep lung. Am. J. Physiol. Lung Cell Mol. Physiol., 293(2):L345-53.
PMID: 17513458

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