Human Endothelin-1 QuantiGlo ELISA Kit

Name: Human Endothelin-1 QuantiGlo ELISA Kit
Brand: R&D Systems
SKU: QET00B
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # QET00B

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Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Urine (100 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Urine
Sensitivity:	0.102 pg/mL
Assay Range:	0.34 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Urine)
Human Endothelin-1 QuantiGlo ELISA Kit Summary:	The QuantiGlo Human Endothelin-1 Chemiluminescent Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Endothelin-1 levels in cell culture supernates, serum, EDTA plasma, and urine without extraction. It contains synthetic human Endothelin-1 and antibodies raised against the synthetic factor. This immunoassay has been shown to accurately quantitate human Endothelin-1. Results obtained using natural human Endothelin-1 showed dose curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Endothelin-1.

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Product Specifications for Human Endothelin-1 QuantiGlo ELISA Kit

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and synthetic human Endothelin-1
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1.78	20.5	90.9	1.76	19.8	86.8
Standard Deviation	0.06	0.7	2.4	0.16	1.3	3.9
CV%	3.4	3.4	2.6	9.1	6.6	4.5

Recovery

The recovery of synthetic human Endothelin-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	93-107
EDTA Plasma (n=4)	94	88-99
Serum (n=4)	95	87-103
Urine (n=4)	98	92-105

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of Endothelin-1 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Endothelin-1 QuantiGlo ELISA Kit

	Human Endothelin-1 Chemiluminescent ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endothelin-1

Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19).

Two receptors for the Endothelin family have been cloned and designated ETA and ETB (20-23). ETA and ETB belong to the large family of heptahelical G protein-coupled receptors. The ETA receptor shows a higher affinity for ET-1 than for ET-2 and lowest affinity for ET-3, while the ETB receptor shows approximately equal affinity for each of the three Endothelins (21, 22, 24). ETA is primarily responsible for the vasoconstrictor effects of ET-1 and is expressed by blood vessel smooth muscle cells (25, 26). The ETB receptor is also present in smooth muscle and the endothelia of blood vessels, kidney, lung, and brain (27). ET-1 has the ability to activate an array of signaling cascades including classical phosphatidylinositol turnover pathways leading to downstream PKC activation and Ca²⁺ mobilization (28-32). Other potential signaling mediators activated or produced by ET-1 include PI 3-kinase/Akt, NO, FAK, and Rho GTPases (32-37). ET-1 signaling may also be mediated indirectly via transactivation of the EGF receptor leading to downstream signaling by Ras and MAP kinases (38, 39). Injection of a single dose of ET-1 produces an initial decrease in systemic blood pressure followed by a prolonged increase in blood pressure (16, 40, 41). Blockade of Endothelin receptors with a systemic injection of an ETA/ETB antagonist causes progressive vasodilation, and elevated levels of ET-1 are found in some forms of human hypertension (42, 43). ET-1 also stimulates cardiac contraction and the growth of cardiac myocytes, regulates the release of vasoactive substances, and stimulates smooth muscle cell mitogenesis (32, 44-46). It also acts as a pro-survival factor for endothelial cells and regulates secretion by hypothalamic and pituitary cells (47, 48). ET-1 may control inflammatory responses by promoting the adhesion and migration of neutrophils and stimulating the production of pro-inflammatory cytokines (49-53). It has also been implicated in cancer progression at several levels including regulating the proliferation and migration of tumor cells and acting as a pro-angiogenic factor (54, 55). In addition, ET-1 has putative roles in other pathologies including septic shock, atherosclerosis, heart failure, renal insufficiency, pulmonary hypertension, and cerebrovascular conditions associated with subarachnoid hemorrhage (15, 56-63).

Item	Value
Entrez Gene IDs	1906 (Human); 13614 (Mouse); 24323 (Rat)
Alternate Names	ARCND3, EDN1, ET1, Endothelin-1, HDLCQ7, PPET1, QME, endothelin 1, preproendothelin-1

Citations for Human Endothelin-1 QuantiGlo ELISA Kit (41)

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Citations are publications that use Bio-Techne products. Selected citations for Human Endothelin-1 QuantiGlo ELISA Kit include:

Species: Human
Sample Types: Serum

M Haffke et al. (2022), Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS) Journal of Translational Medicine, 20(1):138.
PMID: 35317812
Species: Human
Sample Types: Serum

AD Kaze et al. (2022), Association of Plasma Endothelin-1 with Blood Pressure Progression Among African Americans: The Jackson Heart Study American heart journal, 0(0).
PMID: 34986393
Species: Human
Sample Types: Plasma

L van Doorn et al. (2022), Dietary sodium restriction prevents vascular endothelial growth factor inhibitor-induced hypertension British Journal of Cancer, 0(0):1-9.
PMID: 36357702
Species: Human
Sample Types: Serum

N Guo et al. (2021), Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments Frontiers in Pharmacology, 12(0):664320.
PMID: 34194326
Species: Human
Sample Types: Plasma

M Žarak et al. (2021), Adaptive response triggered by the repeated SCUBA diving is reflected in cardiovascular, muscular, and immune biomarkers Physiological Reports, 9(2):e14691.
PMID: 33463896
Species: Human
Sample Types: Serum

Y Chen et al. (2020), Clinical Significance of High-Mobility Group Box 1 Protein (HMGB1) and Nod-Like Receptor Protein 3 (NLRP3) in Patients with Ulcerative Colitis Med. Sci. Monit., 26(0):e919530.
PMID: 31901930
Species: Human
Sample Types: Cell culture supernate

LG Douma et al. (2020), EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells Front Physiol, 11(0):209.
PMID: 32231591
Species: Human
Sample Types: Cell Culture Supernates

X Yang et al. (2020), FURIN Expression in Vascular Endothelial Cells Is Modulated by a Coronary Artery Disease-Associated Genetic Variant and Influences Monocyte Transendothelial Migration J Am Heart Assoc, 9(4):e014333.
PMID: 32067586
Species: Human
Sample Types: Cell Culture Supernates

KL Clines et al. (2018), DKK1 and Kremen Expression Predicts the Osteoblastic Response to Bone Metastasis Transl Oncol, 11(4):873-882.
PMID: 29772510
Species: Mouse
Sample Types: Plasma

BM Fox et al. (2018), Acute Pressor Response to Psychosocial Stress Is Dependent on Endothelium-Derived Endothelin-1 J Am Heart Assoc, 7(4).
PMID: 29453306
Species: Human
Sample Types: Plasma

F Mayyas et al. (2018), Plasma endothelin-1 levels are increased in atrial fibrillation patients with hyperthyroidism PLoS ONE, 13(12):e0208206.
PMID: 30513109
Species: Human
Sample Types: Serum

M Chen et al. (2018), Intervention of integrative medicine treatment has impact on serum levels of ET-1, TNF-?, MLT in RA-CVD Saudi J Biol Sci, 25(5):959-964.
PMID: 30108447
Species: Rat
Sample Types: Plasma

C De Miguel et al. (2017), Endothelin receptor-specific control of endoplasmic reticulum stress and apoptosis in the kidney Sci Rep, 7(0):43152.
PMID: 28230089
Species: Human
Sample Types: Serum

M Lantz et al. (2017), Impaired endothelial function in patients with cryptogenic stroke and patent foramen ovale is not affected by closure J Interv Cardiol, 0(0).
PMID: 28439982
Species: Human
Sample Types: Plasma

Parikh R et al. (2016), Endothelin-1 Predicts Hemodynamically Assessed Pulmonary Arterial Hypertension in HIV Infection. PLoS ONE, 11(1):e0146355.
PMID: 26752193
Jun-Ichi Kira (2016), Allergic Inflammation Leads to Neuropathic Pain via Glial Cell Activation J. Neurosci., 36(47):11929-11945.
PMID: 27881779
Species: Human
Sample Types: Plasma

(2016), Albuminuria Is Associated with Endothelial Dysfunction and Elevated Plasma Endothelin-1 in Sickle Cell Anemia PLoS ONE, 11(9):e0162652.
PMID: 27669006
Species: Rat
Sample Types: Urine

(2016), Free radical scavenging decreases endothelin-1 excretion and glomerular albumin permeability during type 1 diabetes Physiol Rep, 4(24).
PMID: 28039404
Species: Human
Sample Types: Plasma

Malcolm Kohler (2016), Effect of OSA on hypoxic and inflammatory markers during CPAP withdrawal: Further evidence from three randomized control trials Respirology, 0(0).
PMID: 27860068
Species: Rat
Sample Types: Tissue Culture Supernates

Bradley Miller (2016), p66Shc regulates renal vascular tone in hypertension-induced nephropathy J Clin Invest, 0(0).
PMID: 27270176
Species: Rabbit
Sample Types: Plasma

Ranchoux B et al. (2015), Chemotherapy-induced pulmonary hypertension: role of alkylating agents. Am J Pathol, 185(2):356-71.
PMID: 25497573
Species: Human
Sample Types: Serum

Yu A et al. (2015), Association of endothelin-1 and matrix metallopeptidase-9 with metabolic syndrome in middle-aged and older adults. Diabetol Metab Syndr, 7(0):111.
PMID: 26692905
Roksnoer L et al. (2015), Optimum AT1 receptor-neprilysin inhibition has superior cardioprotective effects compared with AT1 receptor blockade alone in hypertensive rats. Kidney Int, 88(1):109-20.
PMID: 25830765
Species: Human
Sample Types: Cell Culture Supernates

Guo F et al. (2014), miR-218 regulates focal adhesion kinase-dependent TGFbeta signaling in fibroblasts. Mol Biol Cell, 25(7):1151-8.
PMID: 24501422
Species: Human
Sample Types: Cell Culture Supernates

Wojciak-Stothard B et al. (2014), Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension. Circulation, 129(17):1770-80.
PMID: 24503951
Species: Human
Sample Types: Cell Culture Supernates

Tannheimer S et al. (2014), The in vitro pharmacology of GS-5759, a novel bifunctional phosphodiesterase 4 inhibitor and long acting beta2-adrenoceptor agonist. J Pharmacol Exp Ther, 349(1):85-93.
PMID: 24513870
Species: Human
Sample Types: Plasma

Botden IP et al. (2011), Daily red wine consumption improves vascular function by a soluble guanylyl cyclase-dependent pathway. Am. J. Hypertens., 24(2):162-8.
PMID: 21088673
Species: Human
Sample Types: Cell Culture Supernates

Guo F et al. (2011), Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair? PLoS ONE, 6(11):e27097.
PMID: 22073262
Species: Rat
Sample Types: Plasma

Loria AS et al. (2010), Early life stress downregulates endothelin receptor expression and enhances acute stress-mediated blood pressure responses in adult rats. Am. J. Physiol. Regul. Integr. Comp. Physiol., 299(1):R185-91.
PMID: 20410476
Species: Human
Sample Types: Plasma

Pham I et al. (2010), Endothelin receptors blockade blunts hypoxia-induced increase in PAP in humans. Eur. J. Clin. Invest., 40(3):195-202.
PMID: 20415698
Species: Human
Sample Types: Serum

Torgrimson BN et al. (2009), Endothelial function, endothelin-1, and fibrinogen in young women using the vaginal contraceptive ring. Fertil. Steril., 92(2):441-7.
PMID: 18706555
Species: Human
Sample Types: Plasma

Casanova G et al. (2009), Effects of nonoral estradiol-micronized progesterone or low-dose oral estradiol-drospirenone therapy on metabolic variables and markers of endothelial function in early postmenopause. Fertil. Steril., 92(2):605-12.
PMID: 18706557
Species: Human
Sample Types: Plasma

Lund AK et al. (2009), Vehicular emissions induce vascular MMP-9 expression and activity associated with endothelin-1-mediated pathways. Arterioscler. Thromb. Vasc. Biol., 29(4):511-7.
PMID: 19150882
Species: Human
Sample Types: Cell Culture Supernates

Carroll VA et al. (2008), Regulation of angiogenic factors by HDM2 in renal cell carcinoma. Cancer Res., 68(2):545-52.
PMID: 18199551
Species: Human
Sample Types: Cell Culture Supernates

Pegorier S et al. (2006), Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells. J. Immunol., 177(7):4861-9.
PMID: 16982928
Species: Equine
Sample Types: Plasma

Menzies-Gow NJ et al. (2005), Digital blood flow and plasma endothelin concentration in clinically endotoxemic horses. Am. J. Vet. Res., 66(4):630-6.
PMID: 15900943
Species: Ovine
Sample Types: Plasma

Kuklin VN et al. (2004), Novel endothelin receptor antagonist attenuates endotoxin-induced lung injury in sheep. Crit. Care Med., 32(3):766-73.
PMID: 15090960
Species: Porcine
Sample Types: Plasma

von Lueder TG et al. (2004), Mechanisms of elevated plasma endothelin-1 in CHF: congestion increases pulmonary synthesis and secretion of endothelin-1. Cardiovasc. Res., 63(1):41-50.
PMID: 15194460
Species: Rat
Sample Types: Tissue Homogenates

Sasser JM et al. (2002), Renal endothelin in chronic angiotensin II hypertension. Am. J. Physiol. Regul. Integr. Comp. Physiol., 283(1):R243-8.
PMID: 12069950
Species: Human
Sample Types: Plasma

Watt G et al. (2001), Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study. QJM, 94(11):599-607.
PMID: 11704689
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Plasma

Reinhart GA et al. (2000), Endothelin and ET(A) receptors in long-term arterial pressure homeostasis in conscious nonhuman primates. 1364165, 279(5):R1701-6.
PMID: 11049852

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Customer Reviews for Human Endothelin-1 QuantiGlo ELISA Kit (3)

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Human Endothelin-1 QuantiGlo ELISA Kit

Name: Anonymous

Sample Tested: Plasma

Verified Customer | Posted 05/24/2019
Human Endothelin-1 QuantiGlo ELISA Kit

Name: Anonymous

Sample Tested: Serum

Verified Customer | Posted 07/24/2017

undiluted human serum from healthy donors
Human Endothelin-1 QuantiGlo ELISA Kit

Name: Anonymous

Sample Tested: EDTA Plasma

Verified Customer | Posted 06/29/2017

Very robust and easy to use kit.

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Human Endothelin-1 QuantiGlo ELISA Kit

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Product Specifications for Human Endothelin-1 QuantiGlo ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Human Endothelin-1 QuantiGlo ELISA Kit

Preparation and Storage

Background: Endothelin-1

Citations for Human Endothelin-1 QuantiGlo ELISA Kit (41)

Customer Reviews for Human Endothelin-1 QuantiGlo ELISA Kit (3)

Customer Images

Human Endothelin-1 QuantiGlo ELISA Kit

Human Endothelin-1 QuantiGlo ELISA Kit

Human Endothelin-1 QuantiGlo ELISA Kit

Product Documents for Human Endothelin-1 QuantiGlo ELISA Kit

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