Human TIMP-4 Quantikine ELISA Kit

Name: Human TIMP-4 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DTM400
Price: 615 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DTM400

Citations (5)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Human Milk (10 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk
Sensitivity:	10 pg/mL
Assay Range:	78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
Human TIMP-4 Quantikine ELISA Kit Summary:	The Quantikine Human TIMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-4 levels in cell culture supernates, serum, plasma, and human milk. It contains Sf 21-expressed recombinant human TIMP-4 and antibodies raised against the recombinant protein. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms. Natural human TIMP-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-4.

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Product Specifications for Human TIMP-4 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	252	793	1476	232	708	1377
Standard Deviation	11	46.9	77.8	20.8	45.8	96.4
CV%	4.4	5.9	5.3	9	6.5	7

Serum, EDTA Plasma, Heparin Plasma, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	235	768	1516	220	712	1414
Standard Deviation	13.2	21.5	70.4	20.2	48.2	87.6
CV%	5.6	2.8	4.6	9.2	6.8	6.2

Recovery

The recovery of TIMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	96	87-105
EDTA Plasma (n=4)	95	87-113
Heparin Plasma (n=4)	93	86-104
Serum (n=4)	96	86-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TIMP-4 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human TIMP-4 Quantikine ELISA Kit

	Human TIMP-4 ELISA Calibrator Diluent RD6-31 Standard Curve
	Human TIMP-4 ELISA Calibrator Diluent RD6-31 (1X) Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-4

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Item	Value
Long Name	Tissue Inhibitors of Metalloproteinases 4
Entrez Gene IDs	7079 (Human); 110595 (Mouse)
Alternate Names	TIMP metallopeptidase inhibitor 4, TIMP-4, TIMP4, Tissue inhibitor of metalloproteinases 4, metalloproteinase inhibitor 4, tissue inhibitor of metalloproteinase 4

Citations for Human TIMP-4 Quantikine ELISA Kit (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human TIMP-4 Quantikine ELISA Kit include:

Species: Human
Sample Types: Plasma

Schumann C et al. (2010), Circulating biomarkers of tissue remodelling in pulmonary hypertension. Biomarkers, 15(0):523.
PMID: 20528622
Species: Human
Sample Types: Plasma

Lamblin N et al. (2010), Profile of macrophages in human abdominal aortic aneurysms: a transcriptomic, proteomic, and antibody protein array study. J. Proteome Res., 9(7):3720-9.
PMID: 20513153
Species: Human
Sample Types: Cell Culture Supernates

Reikvam H et al. (2010), Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 84(3):239-51.
PMID: 19922462
Species: Human
Sample Types: Serum

Kapsoritakis AN et al. (2008), Imbalance of tissue inhibitors of metalloproteinases (TIMP) - 1 and - 4 serum levels, in patients with inflammatory bowel disease. BMC Gastroenterol, 8(1):55.
PMID: 19036126
Species: Human
Sample Types: Plasma

Webb CS et al. (2006), Specific temporal profile of matrix metalloproteinase release occurs in patients after myocardial infarction: relation to left ventricular remodeling. Circulation, 114(10):1020-7.
PMID: 16923753

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.