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Recombinant Human IFN-gamma Protein

Recombinant Human IFN-gamma Protein SDS-PAGE
(2)
Recombinant Human IFN-gamma Protein Bioactivity
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285-IF-100

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285-IF-100/CF
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Key Product Details

Source: E. coli
Accession #: CAA31639
Applications: Bioactivity

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Citations for Recombinant Human IFN-gamma Protein (402)

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Customer Reviews (22)

4.6 out of 5
22 customer ratings
5 stars
68%
4 stars
23%
3 stars
9%
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  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Flow
    Verified Customer | Posted 06/24/2022
    Black -> HLA-E surface expression in T cellsGrey -> HLA-E surface expression in T cells upon IFNg stimulation (12h)
    Recombinant Human IFN-gamma Protein Flow Cytometry 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: Comparing gene expression responses by qPCR after IFNg treatment in immune cell subsets
    Verified Customer | Posted 06/24/2022
    More than a hundred-fold increase for a given target gene in immune cells stimulated with IFNg for 6h compared to controls (for basal level).
    Recombinant Human IFN-gamma Protein In vitro bioactivity in cell culture 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Reason for Rating: Used for successfully stimulating human PBMCs.
    Verified Customer | Posted 06/24/2022
    Lane 1: PBMCs, unstimulatedLane 2: PBMCs, stimulated with IFNg for 6hLane 3: PBMCs, stimulated with IFNg for 24hTarget: HLA detection
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/21/2021
  • Recombinant Human IFN-gamma Protein
    Name: Marija Pieper
    Application: Apoptosis assay
    Verified Customer | Posted 03/11/2021
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Verified Customer | Posted 12/18/2020
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Reason for Rating: Gave consistent M1-like macrophage differentiation of human PBMCs
    Verified Customer | Posted 11/20/2020
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: Easy to solubilize
    Verified Customer | Posted 10/16/2020
    Used the to stimulate ex vivo skin culture
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/09/2020
    HCT-116 cells were treated with hr-IFN-gamma for 24 hours and intracellular IDO-1 expression was measured.
    Recombinant Human IFN-gamma Protein In vitro bioactivity in cell culture 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/08/2020
    Recombinant Human IFN-gamma Protein In vitro bioactivity in cell culture 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/06/2020
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 07/26/2019
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: Consistent results when used for in vitro cell culture
    Verified Customer | Posted 07/25/2019
    CD38 transcript levels measured by qPCR after 24 hours treatment with 10 ng/mL IFN-g in MV-4-11 cells
    Recombinant Human IFN-gamma Protein In vitro bioactivity in cell culture 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: The expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h
    Verified Customer | Posted 06/09/2019
    Recombinant Human IFN-gamma Protein In vitro bioactivity in cell culture 285-IF
  • Name: Mehdi Alavi
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 03/19/2019
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: CellProlif
    Reason for Rating: HT-29 Cell lines were treated with 100 U/ml IFN gamma, after which HLA-DR expression was examined by flow cytometer
    Verified Customer | Posted 11/16/2018
    Recombinant Human IFN-gamma Protein Cell Proliferation 285-IF
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/08/2018
  • Name: Pia Muyot
    Verified Customer | Posted 04/26/2017
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: The IFN-g was excellent in stimulating whole blood.
    Verified Customer | Posted 07/21/2016
    IFN-g was used to stimulate whole blood. 100ng/mL final concentration was used in 1mL whole blood for 15 minutes at 37C. The cells were fixed and stored in -80C until further analysis on CyTOF
  • Recombinant Human IFN-gamma Protein
    Name: Adam Guess
    Application: In vivo study
    Verified Customer | Posted 07/13/2016
    IFN-gamma treatment (solid line) induces HLA-DR expression in cultured MSC as compared to control cells (filled histogram).
    Recombinant Human IFN-gamma Protein In vivo study 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: shu wang
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/31/2016
  • Recombinant Human IFN-gamma Protein
    Name: SOjan Abraham
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016

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FAQs

  • Why does Recombinant Human IFN-gamma protein, Catalog # 285-IF, have the amio acid substitution R160Q?

    The sequence of R&D Systems' rhIFN-gamma, Catalog # 285-IF,  is based on the the following publication in Nature:[https://www.ncbi.nlm.nih.gov/pubmed/?term=Gray+1982+503] Note that in figure 3, the article describes a Gln (Q) in position 160.

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