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Human VEGF Quantikine ELISA Kit

R&D Systems, Inc. a Bio-Techne Brand

Human VEGF ELISA Cell Culture Supernate Standard Curve
(3)
Human VEGF ELISA Serum/Plasma Standard Curve
Human VEGF ELISA Linearity
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PDVE00
SVE00
DVE00

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Setting the standard in quality research reagents

Key Product Details

Assay Length: 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sample Types: Cell Culture Supernates
Sensitivity: 5 pg/mL (Cell Culture Supernates)
9 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Assay Range: 15.6 - 1,000 pg/mL (Cell Culture Supernates)
31.3 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Human VEGF Quantikine ELISA Kit Summary: The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.

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