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Proteome Profiler Human Phospho-RTK Array Kit

R&D Systems, Inc. a Bio-Techne Brand

Contains 4 membranes - each spotted in duplicate with 49 different RTK antibodies

Key Product Details

Detection of Human Receptor Tyrosine Kinase Phosphorylation in KATO-III Cell Line.
(2)
The Human Phospho-RTK Array is specific for ErbB2, ErbB3, and ErbB4 as shown by receptor competition.
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ARY001B
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Citations for Proteome Profiler Human Phospho-RTK Array Kit (251)

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Customer Reviews for Proteome Profiler Human Phospho-RTK Array Kit (7)

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  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: 帆 张
    Verified Customer | Posted 08/31/2021
    Great, excellent, can't wait to start my experiences right away.
    Proteome Profiler Human Phospho-RTK Array Kit ARY001B
  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 07/15/2021
    Purchased to screen changes induced by RTK inhibitors of cancer cell lines. Easy protocol, good clear results which could be independently validated by western blot. The only issue is that R&D are unable to supply information regarding the phospho-site detected, the antibody used in the array or the receptor chain recognised, so you just have to make an educated guess as to which antibody is most appropriate for an independent validation by western blotting etc. Excellent as an initial screen though.
    Proteome Profiler Human Phospho-RTK Array Kit ARY001B
  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 11/18/2019
    I would recommend to my colleagues, nicely performed. (for image acquiring, I used ImageQuant LAS 4000)
    Proteome Profiler Human Phospho-RTK Array Kit ARY001B
  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 03/13/2019
  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 10/29/2018
    Proteome Profiler Human Phospho-RTK Array Kit ARY001B
  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: Jiawan Wang
    Verified Customer | Posted 08/28/2018
    The kit is excellent for p-RTK array in human cell lines. I recommended it to my colleague.
    Proteome Profiler Human Phospho-RTK Array Kit ARY001B
  • Proteome Profiler Human Phospho-RTK Array Kit
    Name: Adam Guess
    Verified Customer | Posted 10/20/2017
    Proteome Profiler Human Phospho-RTK Array Kit ARY001B

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FAQs for Proteome Profiler Human Phospho-RTK Array Kit

  • What are the phosphorylation site(s) of the 49 different tyrosine residues on the RTKs?

    This kit uses a pan anti-phospho-tyrosine antibody as the detection antibody, which means it is capable of detecting phosphorylation at any available tyrosine. It is not designed to be specific for one phosphorylation site on each molecule. For instance, PDGF R alpha has multiple sites of tyrosine phosphorylation (see the “PTM/Processing” section of http://www.uniprot.org/uniprot/P16234 for a list of the currently identified sites). 

  • Can the Human Phospho-RTK Array be used for measuring the relative levels of total RTKs present in a sample?

    No. Although the array kit uses capture antibodies that recognize both phosphorylated and unphosphorylated RTKs, the detection antibody is a pan anti-phospho-tyrosine antibody which only detects phosphorylated tyrosines on activated RTKs.

  • To confirm positive signal for an RTK by IP-Western blot, are the capture antibodies from the Human Phospho-RTK Array Kit offered separately?

    The identities of the capture antibodies on the Human Phospho-RTK Array are considered proprietary. However, to help investigators confirm their array results, Bio-Techne offers antibodies for each RTK detectable in the array kit. These antibodies are validated for Western blot and can be found in our catalog. A pan anti-phospho-tyrosine-HRP detection antibody is also available (Catalog # HAM1676).

Product Documents for Proteome Profiler Human Phospho-RTK Array Kit

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