Which phosphorylated sites are recognized by this assay?

Name: Human Phospho-EphA2 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC4056-2
Price: 440 USD

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-EphA2 DuoSet IC ELISA

Catalog # DYC4056-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Sample Types:	Cell Lysates
Human Phospho-EphA2 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15-(96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Phospho-EphA2 DuoSet IC ELISA

Summary for Human Phospho-EphA2 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human EphA2 in cell lysates. An immobilized capture antibody specific for EphA2 binds both phosphorylated and unphosphorylated EphA2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Human Phospho-EphA2 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Phospho-EphA2 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EphA2

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-A ligands are referred to as EphA. All Eph receptors contain an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail with one conserved tyrosine residue.

Item	Value
Entrez Gene IDs	1969 (Human); 13836 (Mouse)
Alternate Names	ARCC2, EC 2.7.10, EC 2.7.10.1, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, Eck, EphA2, Epithelial cell kinase, Myk2, Sek2, Tyrosine-protein kinase receptor ECK, ephrin type-A receptor 2, soluble EPHA2 variant 1

Citations for Human Phospho-EphA2 DuoSet IC ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-EphA2 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Culture Lysates

M Leite et al. (2020-02-24), Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions Cells, 2020-09(2).
PMID: 32102381
Species: Human
Sample Types: Cell Lysates

C Möser et al. (2018-11-06), Pinpointed Stimulation of EphA2 Receptors via DNA-Templated Oligovalence Int J Mol Sci, 2018-119(11).
PMID: 30404153
Species: Human
Sample Types: Cell Lysates

Hahn AS et al. (2012-06-01), The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus. Nat. Med., 2012-018(6):961-6.
PMID: 22635007
Species: Human
Sample Types: Cell Lysates

Giorgio C et al. (2011-03-30), Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation. PLoS ONE, 2011-06(3):e18128.
PMID: 21479221

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Showing 1 - 4 of 4 citations Showing All

FAQs for Human Phospho-EphA2 DuoSet IC ELISA

Which phosphorylated sites are recognized by this assay?

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-EphA2 DuoSet IC ELISA

Catalog # DYC4056-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Human Phospho-EphA2 DuoSet IC ELISA

Product Specifications for Human Phospho-EphA2 DuoSet IC ELISA

Kit Contents for Human Phospho-EphA2 DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: EphA2

Citations for Human Phospho-EphA2 DuoSet IC ELISA (4)

FAQs for Human Phospho-EphA2 DuoSet IC ELISA

Product Documents for Human Phospho-EphA2 DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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