Which phosphorylated sites are recognized by this assay?

Name: Human Phospho-DDR2 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC6170-2
Rating: 5 (1 reviews)

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-DDR2 DuoSet IC ELISA

Catalog # DYC6170-2 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (1)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell Lysates
Human Phospho-DDR2 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15-(96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Phospho-DDR2 DuoSet IC ELISA

Summary for Human Phospho-DDR2 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human DDR2 in cell lysates. An immobilized capture antibody specific for DDR2 binds both phosphorylated and unphosphorylated DDR2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Human Phospho-DDR2 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Phospho-DDR2 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases. Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain. Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X. Collagens II and V are less efficacious ligands.

DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen. Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 auto-phosphorylation. The ECD of DDR2 exists as a noncovalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding. DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology. Ligand binding induces DDR2 auto-phosphorylation in the cytoplasmic domain, which promotes associations with Shc and Src. In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor auto-phosphorylation. Activation of DDR2 by collagen induces upregulation of MMP1, 2, and 13 as well as DDR2 itself. DDR2 is implicated in collagenous matrix destruction and cell invasiveness. DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia.

Item	Value
Long Name	Discoidin Domain Receptor 2
Entrez Gene IDs	4921 (Human); 18214 (Mouse)
Alternate Names	CD167 antigen-like family member B, CD167b antigen, DDR2, Discoidin domain receptor 2, EC 2.7.10, EC 2.7.10.1, NTRKR3cell migration-inducing protein 20, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, TKT, TKTMIG20a, TYRO10, Trk3, Tyro-10, Tyrosine-protein kinase TYRO10, discoidin domain receptor family, member 2, discoidin domain receptor tyrosine kinase 2, discoidin domain-containing receptor 2, hydroxyaryl-protein kinase, migration-inducing gene 16 protein, neurotrophic tyrosine kinase receptor related 3, tyrosylprotein kinase

Citations for Human Phospho-DDR2 DuoSet IC ELISA (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-DDR2 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

Iwai L et al. (2013-09-15), Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J, 2013-0454(3):501-13.
PMID: 23822953

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Customer Reviews for Human Phospho-DDR2 DuoSet IC ELISA (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Human Phospho-DDR2 DuoSet IC ELISA

Name: Anonymous

Sample Tested: Human cells

Verified Customer | Posted 08/07/2019

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

FAQs for Human Phospho-DDR2 DuoSet IC ELISA

Which phosphorylated sites are recognized by this assay?

This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

Human Phospho-DDR2 DuoSet IC ELISA

Catalog # DYC6170-2 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Summary for Human Phospho-DDR2 DuoSet IC ELISA

Product Specifications for Human Phospho-DDR2 DuoSet IC ELISA

Kit Contents for Human Phospho-DDR2 DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: DDR2

Citations for Human Phospho-DDR2 DuoSet IC ELISA (1)

Customer Reviews for Human Phospho-DDR2 DuoSet IC ELISA (1)

Customer Images

Human Phospho-DDR2 DuoSet IC ELISA

FAQs for Human Phospho-DDR2 DuoSet IC ELISA

Product Documents for Human Phospho-DDR2 DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected