Human Tie-1 DuoSet ELISA

Name: Human Tie-1 DuoSet ELISA
Brand: R&D Systems
SKU: DY5907
Rating: 5 (1 reviews)

Catalog # DY5907 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Tie-1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Tie-1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Tie-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Tie-1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Tie-1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Tie-1 DuoSet ELISA

	Human Tie-1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tie-1

Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). It is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2.

Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

Item	Value
Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
Entrez Gene IDs	7075 (Human); 21846 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, JTK14soluble TIE1 variant 3, TIEsoluble TIE1 variant 4, Tie-1, Tie1, receptor tyrosine kinase, soluble TIE1 variant 1, soluble TIE1 variant 2, soluble TIE1 variant 5, tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1, tyrosine kinase with immunoglobulin-like and EGF-like domains 1, tyrosine-protein kinase receptor Tie-1

Citations for Human Tie-1 DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Tie-1 DuoSet ELISA include:

Species: Human
Sample Types: Plasma

L Tiainen et al. (2019-07-24), High baseline Tie1 level predicts poor survival in metastatic breast cancer BMC Cancer, 2019-019(1):732.
PMID: 31340773
Species: Human
Sample Types: Serum

(2016-08-22), Tie1 controls angiopoietin function in vascular remodeling and inflammation J Clin Invest, 2016-00(0).
PMID: 27548530
Species: Human
Sample Types: Serum

Conroy A et al. (2014-01-20), Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-014(0):35.
PMID: 24444080

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Customer Reviews for Human Tie-1 DuoSet ELISA (1)

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Human Tie-1 DuoSet ELISA

Name: Christian Cassel

Sample Tested: Serum and Plasma

Verified Customer | Posted 10/11/2018

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Product Documents for Human Tie-1 DuoSet ELISA

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Human Tie-1 DuoSet ELISA

Catalog # DY5907 | R&D Systems, Inc. a Bio-Techne Brand

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Summary for Human Tie-1 DuoSet ELISA

Product Specifications for Human Tie-1 DuoSet ELISA

Kit Contents for Human Tie-1 DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human Tie-1 DuoSet ELISA

Preparation and Storage

Background: Tie-1

Citations for Human Tie-1 DuoSet ELISA (3)

Customer Reviews for Human Tie-1 DuoSet ELISA (1)

Human Tie-1 DuoSet ELISA

Product Documents for Human Tie-1 DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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