Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

Name: Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1131-ER
Price: 394 USD

Catalog # 1131-ER | R&D Systems, Inc. a Bio-Techne Brand

Citations (6)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q15303
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

Product Specifications for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human ErbB4/Her4 protein

Human ErbB4 (Gln26-Arg649) Accession # Q15303	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Amino acid sequencing was blocked, suggesting it is consistent with Gln26 as the first N-terminal amino acid

Predicted Molecular Mass

97 kDa (monomer)

SDS-PAGE

122-132 kDa, reducing conditions

Activity

Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 1.5-6 µg/mL in the presence of 10 ng/mL of Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1131-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ErbB4/Her4

ErbB4, also called Her4 (human epidermal growth factor receptor 4), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has been found in ErbB4 and ErbB1 (EGFR). Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25 aa signal sequence, a 626 aa extracellular domain, a 24 aa transmembrane region, and a 633 aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.

Item	Value
References	Plowman, G.D. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1746. Elenius, K. et al. (1997) J. Biol. Chem. 272:26761. Elenius, K. et al. (1999) Oncogene 18:2607. Rio, C. et al. (2000) J. Biol. Chem. 275:10379. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172. Sundaresan, S. et al. (1998) Endocrinology 139:4756. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859. Schlessinger, J. (2000) Cell 103:211. Daly, R.J. (1999) Growth Factors 16:255.
Long Name	Receptor Tyrosine Protein Kinase ErbB4
Entrez Gene IDs	2066 (Human); 13869 (Mouse); 59323 (Rat)
Alternate Names	EC 2.7.10, EC 2.7.10.1, ErbB4, HER4, HER4MGC138404, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4, p180erbB4, receptor tyrosine-protein kinase erbB-4, v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Citations for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Tom J Parry (2016-12-16), Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction Eur. J. Pharmacol, 2016-1796(0):76-89.
PMID: 27993643
Species: Human
Sample Types: Protein
Applications: Direct ELISA, Western Blot

Sachdev S Sidhu (2016-11-22), A Highly Diverse and Functional Naï¿½ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016-10(0).
PMID: 27887869
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Leung K et al. (2015-08-03), A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis. Mol Ther, 2015-023(11):1722-33.
PMID: 26234505
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture)

Iaci JF et al. (2010-08-04), Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke. Neuropharmacology, 2010-059(7):640-9.
PMID: 20691195
Sample Types: Small Molecule
Applications: Dot Blot

Feldwisch J et al. (2010-03-10), Design of an optimized scaffold for affibody molecules. J. Mol. Biol., 2010-0398(2):232-47.
PMID: 20226194
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Hamra FK et al. (2006-11-10), Identification of neuregulin as a factor required for formation of aligned spermatogonia. J. Biol. Chem., 2006-1282(1):721-30.
PMID: 17098736

There are no citations that match your criteria.

Showing 1 - 6 of 6 citations Showing All

Product Documents for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

Catalog # 1131-ER | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased