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Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA
Catalog # DYC2724-2 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Type: | Solid Phase Sandwich ELISA |
| Sample Types: | Cell Lysates |
| Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Features: |
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Figure 1. The Human Phospho-VEGF R3 DuoSet IC ELISA is more sensitive than immunoprecipitation (IP)-Western analysis Lysates prepared from human VEGF R3 transfected NS0 cells (NS0-hVEGF R3) were diluted in a series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-VEGF R3 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-VEGF R3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (Catalog # AR004). Human Phospho-VEGF R3 can be detected in this DuoSet IC ELISA by using approximately 10 to 15 times less lysate than is needed for a conventional IP-Western blot. |
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Figure 2. The specificity of the Human Phospho-VEGF R3 DuoSet IC ELISA is confirmed by receptor competition The indicated amounts of recombinant extracellular domains of human VEGF R3 (Catalog #349-F4), human VEGF R1 (Catalog #321-FL), human VEGF R2 (Catalog #357-KD) or human Tie-2 (Catalog #313-TI) were added to 20 μg NS0-hVEGF R3 cell lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human VEGF R3. |
Citations for Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA (4)
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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA include:
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Species: Human
Sample Types: Cell Culture Supernates
A Dumond et al. (2021-05-17), Anti-Vascular Endothelial Growth Factor C Antibodies Efficiently Inhibit the Growth of Experimental Clear Cell Renal Cell Carcinomas Cells, 2021-010(5).
PMID: 34067671 -
Species: Human
Sample Types: Cell Lysates
(2016-05-09), Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD J Clin Invest, 2016-00(0).
PMID: 27159393 -
Species: Human
Sample Types: Cell Lysates
Harris et al. (2013-02-12), The propeptides of VEGF-D determine heparin binding, receptor heterodimerization, and effects on tumor biology. J Biol Chem, 2013-0288(12):8176-86.
PMID: 23404505 -
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Schomber T et al. (2009-01-01), Differential effects of the vascular endothelial growth factor receptor inhibitor PTK787/ZK222584 on tumor angiogenesis and tumor lymphangiogenesis. Mol. Cancer Ther., 2009-08(1):55-63.
PMID: 19139113
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Customer Reviews for Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA (1)
FAQs for Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA
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Which phosphorylated sites are recognized by this assay?
This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
