Detects proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. The antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Phospho-Tyrosine Biotinylated Antibody

Detection of Phospho-PDGF R beta by ELISA.

Human Foreskin Fibroblasts were untreated or treated with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent.

Detection of Phospho-Tyrosine by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Applications for Phospho-Tyrosine Biotinylated Antibody

Application

Recommended Usage

ELISA

0.75 µg/mL
Sample: Proteins containing phosphorylated tyrosine residues

Western Blot

1 µg/mL
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Phospho-Tyrosine Biotinylated Antibody

For research use only

Citations
Reviews

Phospho-Tyrosine Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1676

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications