Key Product Details
Product Summary for Phospho-Tyrosine Biotinylated Antibody
Scientific Data Images for Phospho-Tyrosine Biotinylated Antibody
Detection of Phospho-PDGF R beta by ELISA.Human Foreskin Fibroblasts were untreated or treated with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent.
Detection of Phospho-Tyrosine by Western Blot.Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.
Applications for Phospho-Tyrosine Biotinylated Antibody
Sample: Proteins containing phosphorylated tyrosine residues
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Read 3 publications using BAM1676 in the following applications:
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Specific Notices for Phospho-Tyrosine Biotinylated Antibody
For research use only