Phospho-Tyrosine Biotinylated Antibody
Catalog # BAM1676 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Phospho-Tyrosine Biotinylated Antibody
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Phospho-Tyrosine Biotinylated Antibody
Detection of Phospho-PDGF R beta by ELISA.
Human Foreskin Fibroblasts were untreated or treated with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent.Detection of Phospho-Tyrosine by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.Applications for Phospho-Tyrosine Biotinylated Antibody
ELISA
Sample: Proteins containing phosphorylated tyrosine residues
Western Blot
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Published Applications
Read 3 publications using BAM1676 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Phospho-Tyrosine
Alternate Names
Product Documents for Phospho-Tyrosine Biotinylated Antibody
Product Specific Notices for Phospho-Tyrosine Biotinylated Antibody
For research use only
Citations for Phospho-Tyrosine Biotinylated Antibody (3)
Citations are publications that use Bio-Techne products. Selected citations for Phospho-Tyrosine Biotinylated Antibody include:
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Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotC Yilmaz et al. (2022-03-09), ENT-A010, a Novel Steroid Derivative, Displays Neuroprotective Functions and Modulates Microglial Responses Biomolecules, 2022-012(3).
PMID: 35327616 -
Species: Human,Rat
Sample Types: Cell Lysates
Applications: Western BlotT Rogdakis et al. (2022-03-06), Development and Biological Characterization of a Novel Selective TrkA Agonist with Neuroprotective Properties against Amyloid Toxicity Biomedicines, 2022-010(3).
PMID: 35327415 -
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development (Detection)Reinhard M et al. (2013-04-04), Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study. BMC Nephrol, 2013-014(0):80.
PMID: 23557110
There are no citations that match your criteria.
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