Phospho-Tyrosine HRP-conjugated Antibody

Name: Phospho-Tyrosine HRP-conjugated Antibody
Brand: R&D Systems
SKU: HAM1676

Catalog # HAM1676 | R&D Systems, Inc. a Bio-Techne Brand

Citations (8)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Multi-Species

Applications

ELISA, Western Blot

Label

HRP

Antibody Source

Monoclonal Mouse IgG₁ Clone # 179003

Product Summary for Phospho-Tyrosine HRP-conjugated Antibody

Specificity

Detects endogenous proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Phospho-Tyrosine HRP-conjugated Antibody

Detection of Phospho-Tyrosine by Western Blot.

Phospho-PDGF R beta ELISA Using HAM1676 as Detection Antibody.

Applications for Phospho-Tyrosine HRP-conjugated Antibody

Application

Recommended Usage

ELISA

Western Blot

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 8 publications using HAM1676 in the following applications:

ELISA (1 Publication)
ELISA Development (Detection) (1 Publication)
Western Blot (6 Publications)

Formulation, Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Product Documents for Phospho-Tyrosine HRP-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Phospho-Tyrosine HRP-conjugated Antibody

For research use only

Citations for Phospho-Tyrosine HRP-conjugated Antibody (8)

Showing 1 - 8 of 8 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Phospho-Tyrosine HRP-conjugated Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

O Contreras et al. (2021-05-08), PDGF-PDGFR network differentially regulates the fate, migration, proliferation, and cell cycle progression of myogenic cells Cellular Signalling, 2021-084(0):110036.
PMID: 33971280
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development (Detection)

Q Haas et al. (2019-04-15), Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment Cancer Immunol Res, 2019-00(0).
PMID: 30988027
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA

J Li et al. (2018-02-14), Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs Adv Healthc Mater, 2018-00(0).
PMID: 29441702
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016-12-23), Interaction of cytokeratin 19 head domain and HER2 in the cytoplasm leads to activation of HER2-Erk pathway Sci Rep, 2016-16(0):39557.
PMID: 28008968
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Shang X et al. (2014-10-25), Claudin-4 controls the receptor tyrosine kinase EphA2 pro-oncogenic switch through ss-catenin. Cell Commun Signal, 2014-112(1):59.
PMID: 25344320
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Surls J et al. (2012-06-19), Increased membrane cholesterol in lymphocytes diverts T-cells toward an inflammatory response. PLoS ONE, 2012-07(6):e38733.
PMID: 22723880
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sarup J et al. (2008-10-01), Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts. Mol. Cancer Ther., 2008-17(10):3223-36.
PMID: 18852126
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Chaudhari A et al. (2007-01-24), Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth. Biochemistry, 2007-046(7):1799-810.
PMID: 17249693

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