Human Total PDGF R beta DuoSet IC ELISA

Name: Human Total PDGF R beta DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC385-2
Price: 440 USD

Catalog # DYC385-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	250 - 16,000 pg/mL
Sample Types:	Cell Lysates
Human Total PDGF R beta DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total PDGF R beta DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total PDGF R beta in cell lysates. An immobilized capture antibody specific for PDGF R beta binds both phosphorylated and unphosphorylated PDGF R beta. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total PDGF R beta DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total PDGF R beta DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total PDGF R beta DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF R beta

PDGF R beta is a transmembrane receptor tyrosine kinase (RTK) that primarily binds to the PDGF-BB isoform of the growth factor. Ligand binding induces receptor homodimerization or heterodimerization with PDGF R alpha followed by autophosphorylation and signal transduction. PDGF receptor signaling is attenuated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. The expression of the alpha and beta receptors is independently regulated in various cell types. A soluble splice variant of PDGF R beta binds PDGF with high affinity and functions as a potent PDGF antagonist.

Item	Value
Long Name	Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs	5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names	CD140 antigen-like family member B, CD140b, CD140b antigen, EC 2.7.10, EC 2.7.10.1, JTK12, PDGF R beta, PDGF-R-beta, PDGFR, PDGFR1, PDGFRB, beta-type platelet-derived growth factor receptor, platelet-derived growth factor receptor, beta polypeptide

Citations for Human Total PDGF R beta DuoSet IC ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total PDGF R beta DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

Aderhold C et al. (2013-12-01), Small molecule-based chemotherapeutic approach in p16-positive and -negative HNSCC in vitro. Anticancer Res, 2013-133(12):5385-93.
PMID: 24324073
Species: Human
Sample Types: Cell Lysates

Evans IM et al. (2011-01-18), Neuropilin-1 signaling through p130Cas tyrosine phosphorylation is essential for growth factor-dependent migration of glioma and endothelial cells. Mol. Cell. Biol., 2011-031(6):1174-85.
PMID: 21245381

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Product Documents for Human Total PDGF R beta DuoSet IC ELISA

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