Human Total EphA2 DuoSet IC ELISA

Name: Human Total EphA2 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC3035-2

Catalog # DYC3035-2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

Discontinued Product

DYC3035-2 has been discontinued. View all EphA2 products.

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	78.1 - 5,000 pg/mL
Sample Types:	Cell Lysates
Human Total EphA2 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Total EphA2 DuoSet IC ELISA

Technical Data
Citations (3) / Reviews

Summary for Human Total EphA2 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total EphA2 in cell lysates. An immobilized capture antibody specific for EphA2 binds both phosphorylated and unphosphorylated EphA2. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total EphA2 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total EphA2 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EphA2

Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-A ligands are referred to as EphA. All Eph receptors contain an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail with one conserved tyrosine residue.

Item	Value
Entrez Gene IDs	1969 (Human); 13836 (Mouse)
Alternate Names	ARCC2, EC 2.7.10, EC 2.7.10.1, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, Eck, EphA2, Epithelial cell kinase, Myk2, Sek2, Tyrosine-protein kinase receptor ECK, ephrin type-A receptor 2, soluble EPHA2 variant 1

Citations for Human Total EphA2 DuoSet IC ELISA (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Total EphA2 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

Q Wei et al. (2020-07-28), EphA2?enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer Molecular Medicine Reports, 2020-00(0).
PMID: 32945400
Species: Human
Sample Types: Plasma

TK Darling et al. (2020-01-30), EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria PLoS Pathog., 2020-016(1):e1008261.
PMID: 31999807
Species: Human
Sample Types: Tissue Homogenates

Gordon K et al. (2012-11-29), Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis. J Invest Dermatol, 2012-1133(3):712-22.
PMID: 23190894

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Product Documents for Human Total EphA2 DuoSet IC ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS